Science | Nature | Cell | View More
Natural Products
Euphorbiasteroid
Euphorbiasteroid
ChemFaces products have been cited in many studies from excellent and top scientific journals
Product Name Euphorbiasteroid
Price: $100 / 20mg
CAS No.: 28649-59-4
Catalog No.: CFN90641
Molecular Formula: C32H40O8
Molecular Weight: 552.66 g/mol
Purity: >=98%
Type of Compound: Diterpenoids
Physical Desc.: Powder
Source: The herbs of Euphorbia pekinensis Rupr.
Solvent: Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.
Download: COA    MSDS    SDF
Similar structural: Comparison
Guestbook:
Contact Us
Order & Inquiry & Tech Support

Tel: (0086)-27-84237683
Tech: service@chemfaces.com
Order: manager@chemfaces.com
Address: 176, CheCheng Eest Rd., WETDZ, Wuhan, Hubei 430056, PRC
How to Order
Orders via your E-mail:

1. Product number / Name / CAS No.
2. Delivery address
3. Ordering/billing address
4. Contact information
Order: manager@chemfaces.com
Delivery time
Delivery & Payment method

1. Usually delivery time: Next day delivery by 9:00 a.m. Order now

2. We accept: Wire transfer & Credit card & Paypal
Citing Use of our Products
* Packaging according to customer requirements(5mg, 10mg, 20mg and more). We shipped via FedEx, DHL, UPS, EMS and others courier.
According to end customer requirements, ChemFaces provide solvent format. This solvent format of product intended use: Signaling Inhibitors, Biological activities or Pharmacological activities.
Size /Price /Stock 10 mM * 1 mL in DMSO / $18.7 / In-stock
Other Packaging *Packaging according to customer requirements(100uL/well, 200uL/well and more), and Container use Storage Tube With Screw Cap
Our products had been exported to the following research institutions and universities, And still growing.
  • University of Eastern Finland (Finland)
  • University of Ioannina (Greece)
  • University of Toronto (Canada)
  • Universidade do Porto (Portugal)
  • Chiang Mai University (Thailand)
  • University of Hull (United Kingdom)
  • Sanford Burnham Medical Researc... (USA)
  • Shanghai Institute of Biochemis... (China)
  • Institute of Bioorganic Chemist... (Poland)
  • University Medical Center Mainz (Germany)
  • Helmholtz Zentrum München (Germany)
  • More...
Package
Featured Products
Senkyunolide A

Catalog No: CFN99594
CAS No: 62006-39-7
Price: $90/20mg
Shikonin

Catalog No: CFN99907
CAS No: 517-88-4
Price: $50/20mg
Ginsenoside Rf

Catalog No: CFN99976
CAS No: 52286-58-5
Price: $188/20mg
Senkirkine

Catalog No: CFN00424
CAS No: 2318-18-5
Price: $ /
Biorobin

Catalog No: CFN97804
CAS No: 17297-56-2
Price: $ /
Nimbolide

Catalog No: CFN91919
CAS No: 25990-37-8
Price: $318/10mg
Isoliquiritin

Catalog No: CFN99155
CAS No: 5041-81-6
Price: $100/20mg
Exoticin

Catalog No: CFN99417
CAS No: 13364-94-8
Price: $463/5 mg
Apigenin

Catalog No: CFN98843
CAS No: 520-36-5
Price: $40/20mg
Padmatin

Catalog No: CFN97280
CAS No: 80453-44-7
Price: $268/5mg
Related Screening Libraries
Size /Price /Stock 10 mM * 100 uL in DMSO / Inquiry / In-stock
10 mM * 1 mL in DMSO / Inquiry / In-stock
Related Libraries
Biological Activity
Description: Euphorbiasteroid has anti-obesity activities, it inhibits adipogenesis of 3T3-L1 cells through activation of the AMPK pathway. Euphorbiasteroid could be a transport substrate for P-gp that can effectively inhibit P-gp-mediated drug transport and reverse resistance to anticancer drugs in MES-SA/Dx5 cells; it induces HL-60 cells to apoptosis via promoting Bcl-2/Bax apoptotic signaling pathway in a dose-dependent manner.
Targets: AMPK | P-gp | Bcl-2/Bax | ATPase
In vitro:
Cell Biochem Funct. 2015 Jun;33(4):220-5.
Euphorbiasteroid, a component of Euphorbia lathyris L., inhibits adipogenesis of 3T3-L1 cells via activation of AMP-activated protein kinase.[Pubmed: 25914364]
The purpose of this study is to investigate the effects of Euphorbiasteroid, a component of Euphorbia lathyris L., on adipogenesis of 3T3-L1 pre-adipocytes and its underlying mechanisms.
METHODS AND RESULTS:
Euphorbiasteroid decreased differentiation of 3T3-L1 cells via reduction of intracellular triglyceride (TG) accumulation at concentrations of 25 and 50 μM. In addition, Euphorbiasteroid altered the key regulator proteins of adipogenesis in the early stage of adipocyte differentiation by increasing the phosphorylation of AMP-activated protein kinase (AMPK) and acetyl-CoA carboxylase. Subsequently, levels of adipogenic proteins, including fatty acid synthase, peroxisome proliferator-activated receptor-γ and CCAAT/enhancer-binding protein α, were decreased by Euphorbiasteroid treatment at the late stage of adipocyte differentiation. The anti-adipogenic effect of Euphorbiasteroid may be derived from inhibition of early stage of adipocyte differentiation.
CONCLUSIONS:
Taken together, Euphorbiasteroid inhibits adipogenesis of 3T3-L1 cells through activation of the AMPK pathway. Therefore, Euphorbiasteroid and its source plant, E. lathyris L., could possibly be one of the fascinating anti-obesity agent.
Phytother Res. 2010 Jul;24(7):1042-6.
Euphorbiasteroid reverses P-glycoprotein-mediated multi-drug resistance in human sarcoma cell line MES-SA/Dx5.[Pubmed: 19960428 ]

METHODS AND RESULTS:
In this study, we evaluated whether Euphorbiasteroid isolated from Euphorbia lathyris has the potential to reverse P-glycoprotein (P-gp)-mediated multi-drug resistance (MDR) by using the drug-sensitive human sarcoma cell line MES-SA and its MDR counterpart MES-SA/Dx5. Interestingly, even at low concentrations of Euphorbiasteroid (1-3 microM), it efficiently restored the toxicities of anticancer drugs including vinblastine, taxol and doxorubicin in MES-SA/Dx5 cells. Additionally, the computational Bayesian model for predicting potential P-gp substrates or inhibitors revealed that Euphorbiasteroid showed 97% probability for substrate likeness having similar molecular features with 50 P-gp substrates. Consistent with this result, the substrate likeness of Euphorbiasteroid was also experimentally confirmed by P-gp ATPase activity assay.
CONCLUSIONS:
In conclusion, our finding suggested that Euphorbiasteroid could be a transport substrate for P-gp that can effectively inhibit P-gp-mediated drug transport and reverse resistance to anticancer drugs in MES-SA/Dx5 cells.
Euphorbiasteroid Description
Source: The herbs of Euphorbia pekinensis Rupr.
Solvent: Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.
Storage: Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).

Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

Need more advice on solubility, usage and handling? Please email to: service@chemfaces.com

After receiving: The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
ChemFaces New Products and Compounds
Indolelactic acid

Catalog No: CFN95263
CAS No: 1821-52-9
Price: $100/20mg
Fargesol

Catalog No: CFN95027
CAS No: 128855-64-1
Price: $318/20mg
3-Hydroxymollugin

Catalog No: CFN95424
CAS No: 154706-45-3
Price: $368/5mg
Euphorbia factor L24

Catalog No: CFN95334
CAS No: 1613700-13-2
Price: $318/5mg
Mahuannin E

Catalog No: CFN95585
CAS No: 1173887-70-1
Price: $318/5mg
Norwogonin-8-O-glucuronide

Catalog No: CFN95423
CAS No: 118525-47-6
Price: $368/5mg
Obovatol

Catalog No: CFN95054
CAS No: 83864-78-2
Price: $318/5mg
6''-O-Acetylsaikosaponin D

Catalog No: CFN95090
CAS No: 64340-45-0
Price: $288/5mg
Recently, ChemFaces products have been cited in many studies from excellent and top scientific journals

Cell. 2018 Jan 11;172(1-2):249-261.e12.
doi: 10.1016/j.cell.2017.12.019.
IF=36.216(2019)

PMID: 29328914

Cell Metab. 2020 Mar 3;31(3):534-548.e5.
doi: 10.1016/j.cmet.2020.01.002.
IF=22.415(2019)

PMID: 32004475

Mol Cell. 2017 Nov 16;68(4):673-685.e6.
doi: 10.1016/j.molcel.2017.10.022.
IF=14.548(2019)

PMID: 29149595

ACS Nano. 2018 Apr 24;12(4): 3385-3396.
doi: 10.1021/acsnano.7b08969.
IF=13.903(2019)

PMID: 29553709

Nature Plants. 2016 Dec 22;3: 16206.
doi: 10.1038/nplants.2016.205.
IF=13.297(2019)

PMID: 28005066

Sci Adv. 2018 Oct 24;4(10): eaat6994.
doi: 10.1126/sciadv.aat6994.
IF=12.804(2019)

PMID: 30417089
Calculate Dilution Ratios(Only for Reference)
1 mg 5 mg 10 mg 20 mg 25 mg
1 mM 1.8094 mL 9.0472 mL 18.0943 mL 36.1886 mL 45.2358 mL
5 mM 0.3619 mL 1.8094 mL 3.6189 mL 7.2377 mL 9.0472 mL
10 mM 0.1809 mL 0.9047 mL 1.8094 mL 3.6189 mL 4.5236 mL
50 mM 0.0362 mL 0.1809 mL 0.3619 mL 0.7238 mL 0.9047 mL
100 mM 0.0181 mL 0.0905 mL 0.1809 mL 0.3619 mL 0.4524 mL
* Note: If you are in the process of experiment, it's need to make the dilution ratios of the samples. The dilution data of the sheet for your reference. Normally, it's can get a better solubility within lower of Concentrations.
Protocol
Cell Research:
Chinese Journal of Cancer Prevention & Treatment, 2014 , 21 (23) :1865-1870.
Mechanism of euphorbiasteroid inducing apoptosis of HL-60 cells[Reference: WebLink]
To investigate the effect of Euphorbiasteroid on inducing the apoptosis of HL-60 cells and identify the role of Bcl-2/Bax signaling pathway in this process.
METHODS AND RESULTS:
HL-60 cells were treated with low, middleand high dose of Euphorbiasteroid in vitro for 24 h and after that cell counting Kit-8 was used to detect cell proliferation. The morphology of HL-60 cells was observed under light and fluorescent microscopy. The early cell apoptosis was detected using flow cytometry with Annexin V/PI double staining. The expressions of Bcl-2, Bax, Caspase-9, Caspase-3 mRNA were analyzed by RT-PCR. The activity of Caspase-9, 3 was examined by chromatometry, ELISA detect Caspase-9, Caspase-3 activity of protein. HL-60 cell proliferation was inhibited significantly by Euphorbiasteroid administration (F=42.97, P<0.001). Comparison between different concentrations, the difference was statistically significant, P<0.05. Early cell apoptosis rate of HL-60 cells was enhanced markedly after Euphorbiasteroid administration (F=56.74, P<0.001), after 10, 20, 40 μg/mL Euphorbiasteroid treatment for 24 h, the HL-60 cells early apoptosis rate respectively were(23.4±3.1)%, (35.7±4.3)%, (53.2±3.9)%, and cells presented typical apoptosis morphological changes. Bax mRNA transcription level increased significantly, the Bcl-2 mRNA transcription level decreased significantly (F=53.45, P<0.001) after Euphorbiasteroid administration in a dose-dependent manner. Caspase-9, Caspase-3 mRNA were up-regulated in the transcriptional level, and a compound dose dependent (F=34.21, P<0.001), semen euphorbiae sterol effect on the HL-60 cells after 24 h Caspase-9, 3 protein activity significantly increased (F=54.33, P<0.001), with the increase of concentration of semen euphorbiae sterol the protein activity increased.
CONCLUSIONS:
Euphorbiasteroid induces HL-60 cells to apoptosis via promoting Bcl-2/Bax apoptotic signaling pathway in a dose-dependent manner.
Senkyunolide A

Catalog No: CFN99594
CAS No: 62006-39-7
Price: $90/20mg
Shikonin

Catalog No: CFN99907
CAS No: 517-88-4
Price: $50/20mg
Ginsenoside Rf

Catalog No: CFN99976
CAS No: 52286-58-5
Price: $188/20mg
Senkirkine

Catalog No: CFN00424
CAS No: 2318-18-5
Price: $ /
Biorobin

Catalog No: CFN97804
CAS No: 17297-56-2
Price: $ /
Nimbolide

Catalog No: CFN91919
CAS No: 25990-37-8
Price: $318/10mg
Isoliquiritin

Catalog No: CFN99155
CAS No: 5041-81-6
Price: $100/20mg
Exoticin

Catalog No: CFN99417
CAS No: 13364-94-8
Price: $463/5 mg
Apigenin

Catalog No: CFN98843
CAS No: 520-36-5
Price: $40/20mg
Padmatin

Catalog No: CFN97280
CAS No: 80453-44-7
Price: $268/5mg
Tags: buy Euphorbiasteroid | Euphorbiasteroid supplier | purchase Euphorbiasteroid | Euphorbiasteroid cost | Euphorbiasteroid manufacturer | order Euphorbiasteroid | Euphorbiasteroid distributor