L-Valine | CAS:72-18-4 | Manufacturer ChemFaces

L-Valine

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According to end customer requirements, ChemFaces provide solvent format. This solvent format of product intended use: Signaling Inhibitors, Biological activities or Pharmacological activities.

Size /Price /Stock	10 mM * 1 mL in DMSO / $7.0 / In-stock
Other Packaging	*Packaging according to customer requirements(100uL/well, 200uL/well and more), and Container use Storage Tube With Screw Cap

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Description:

L-Valine is an essential amino acid for the human body. L-Valine and L-isoleucine could be used in the supplements of low-protein corn and soybean meal all-vegetable diets for broilers.

In vitro:

Appl Environ Microbiol. 2007 Apr;73(7):2079-84.

L-valine production with pyruvate dehydrogenase complex-deficient Corynebacterium glutamicum.[Pubmed: 17293513 ]

METHODS AND RESULTS:
Corynebacterium glutamicum was engineered for the production of L-Valine from glucose by deletion of the aceE gene encoding the E1p enzyme of the pyruvate dehydrogenase complex and additional overexpression of the ilvBNCE genes encoding the L-Valine biosynthetic enzymes acetohydroxyacid synthase, isomeroreductase, and transaminase B. In the absence of cellular growth, C. glutamicum DeltaaceE showed a relatively high intracellular concentration of pyruvate (25.9 mM) and produced significant amounts of pyruvate, L-alanine, and L-Valine from glucose as the sole carbon source. Lactate or acetate was not formed. Plasmid-bound overexpression of ilvBNCE in C. glutamicum DeltaaceE resulted in an approximately 10-fold-lower intracellular pyruvate concentration (2.3 mM) and a shift of the extracellular product pattern from pyruvate and L-alanine towards L-Valine.
CONCLUSIONS:
In fed-batch fermentations at high cell densities and an excess of glucose, C. glutamicum DeltaaceE(pJC4ilvBNCE) produced up to 210 mM L-Valine with a volumetric productivity of 10.0 mM h(-1) (1.17 g l(-1) h(-1)) and a maximum yield of about 0.6 mol per mol (0.4 g per g) of glucose.

Biotechnol Bioeng. 2005 Aug 5;91(3):356-68.

Application of model discriminating experimental design for modeling and development of a fermentative fed-batch L-valine production process.[Pubmed: 15984033]

A model discriminating experimental design approach for fed-batch processes has been developed and applied to the fermentative production of L-Valine by a genetically modified Corynebacterium glutamicum strain possessing multiple auxotrophies as an example.
METHODS AND RESULTS:
Being faced with the typical situation of uncertain model information based on preliminary experiments, model discriminating design was successfully applied to improve discrimination between five competing models. Within the same modeling and experimental design framework, also the planning of an optimized production process with respect to the total volumetric productivity is shown. Simulation results were experimentally affirmed, yielding an increased total volumetric productivity of 6.2 mM L-Valine per hour.
CONCLUSIONS:
However, also so far unknown metabolic mechanisms were observed in the optimized process, underlining the importance of process optimization during modeling to avoid problems of extreme extrapolation of model predictions during the final process optimization.

L-Valine Description

Source:	The cultures of Corynebacterium glutamicum.
Solvent:	DMSO, Pyridine, Methanol, Ethanol, etc.
Storage:	Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C). Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour. Need more advice on solubility, usage and handling? Please email to: service@chemfaces.com
After receiving:	The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.

Product Name	L-Valine
Price:	$30 / 20mg
CAS No.:	72-18-4
Catalog No.:	CFN94831
Molecular Formula:	C₅H₁₁NO₂
Molecular Weight:	117.1 g/mol
Purity:	>=98%
Type of Compound:	Alkaloids
Physical Desc.:	Powder
Source:	The cultures of Corynebacterium glutamicum.
Solvent:	DMSO, Pyridine, Methanol, Ethanol, etc.
Download:	COA MSDS
Similar structural:	Comparison
Guestbook:

Size /Price /Stock	10 mM * 100 uL in DMSO / Inquiry / In-stock 10 mM * 1 mL in DMSO / Inquiry / In-stock
Related Libraries	Dietary supplement Compound Library Alkaloids Compound Library

	1 mg	5 mg	10 mg	20 mg	25 mg
1 mM	8.5397 mL	42.6985 mL	85.3971 mL	170.7942 mL	213.4927 mL
5 mM	1.7079 mL	8.5397 mL	17.0794 mL	34.1588 mL	42.6985 mL
10 mM	0.854 mL	4.2699 mL	8.5397 mL	17.0794 mL	21.3493 mL
50 mM	0.1708 mL	0.854 mL	1.7079 mL	3.4159 mL	4.2699 mL
100 mM	0.0854 mL	0.427 mL	0.854 mL	1.7079 mL	2.1349 mL

CFN97119	Cerbinal	65597-42-4	204.2	C₁₁H₈O₄
CFN93272	Platycodin D3	67884-03-1	1387.47	C₆₃H₁₀₂O₃₃
CFN92042	Petunidin-3-O-galactoside chloride	28500-02-9	514.9	C₂₂H₂₃ClO₁₂
CFN99109	Senegenin	2469-34-3	537.14	C₃₀H₄₅ClO₆