Polyphyllin G | CAS:76296-75-8 | Manufacturer ChemFaces

Polyphyllin G

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Description:

Polyphyllin G has been shown to have strong anticancer activities in a wide variety of human cancer cell lines, it also shows signiﬁcant anthelmintic activity against Dactylogyrus intermedius with EC50 values of 1.2 mg L(-1) and the acute toxicities (LC50) values of 2.9 mg L(-1).

Targets:

In vitro:

Phytomedicine. 2016 Dec 1;23(13):1545-1554.

Polyphyllin G induces apoptosis and autophagy cell death in human oral cancer cells.[Pubmed: 27823618]

Polyphyllin G (also called polyphyllin VII), extract from rhizomes of Paris yunnanensis Franch, has been shown to have strong anticancer activities in a wide variety of human cancer cell lines. However, the underlying influences of autophagy in human oral squamous cell carcinoma (OSCC) remain unclear.
METHODS AND RESULTS:
In this study, the roles of apoptosis and autophagy in Polyphyllin G-induced death in human oral cancer cells were investigated. Moreover, the molecular mechanism of the anticancer effects of Polyphyllin G in human oral cancer cells was investigated. The results revealed that Polyphyllin G significantly inhibited cell proliferation in human oral cancer cells; it dose-dependently induced apoptosis in SAS and OECM-1 cells through caspase-3, -8, and -9 activation and poly (ADP-ribose) polymerase cleavage. In addition, changes were observed in Bcl-2 and proapoptosis-related protein expression in different human oral cancer cell lines. The expression of both LC3-II and beclin-1 was markedly increased, suggesting the induction of autophagy in Polyphyllin G-treated oral cells. To further clarify whether Polyphyllin G-induced apoptosis and autophagy depended on Akt/extracellular signal-regulated kinases (ERK)/c-Jun N-terminal kinases (JNK)/p38 mitogen-activated protein kinases (MAPK) signaling pathways, the cells were cotreated with inhibitors. The results demonstrated Polyphyllin G-induced apoptosis in oral cells through the activation of ERK, Akt, p38 MAPK, and JNK, whereas ERK and JNK accounted for Polyphyllin G-induced autophagy.
CONCLUSIONS:
This study is the first to demonstrate apoptosis and autophagy during Polyphyllin G-induced cell death in human oral cancer cell lines. These results suggest that Polyphyllin G is a promising candidate for developing antitumor drugs targeting human oral squamous cell carcinoma.

Arch Biochem Biophys . 2018 Apr 15;644:93-99.

Polyphyllin G exhibits antimicrobial activity and exerts anticancer effects on human oral cancer OECM-1 cells by triggering G2/M cell cycle arrest by inactivating cdc25C-cdc2[Pubmed: 29352966]

Abstract Plant natural products have long been considered to be important sources of bioactive molecules. A large number of antimicrobial and anticancer agents have been isolated form plants. In the present study we evaluated the antimicrobial and anticancer activity of a plant derived secondery metabolite, Polyphyllin G. The results of antibacterial assays showed that Polyphyllin G prevented the growth of both Gram-positive and Gram-negative bacteria with minimum inhibitory concentrations (MICs) ranging from 13.1 to 78 μg/ml. Antifungal activity measured as inhibition of mycelium growth ranged between 38.32 and 56.50%. Further Polyphyllin G was also evaluated against a panel of cancer cell lines. The IC50 of Polyphyllin G ranged from 10 to 65 μM. However the IC50 of Polyphyllin G was found to be comparatively high (120 μM) against the normal FR2 cancer cell line. The lowest IC50 of 10 μM was found against the oral cancer cell line OECM-1. Therefore further studies were carried out on this cell line only. Our results indicated that Polyphyllin G induced cell arrest in oral cancer OECM-1 cells by inactivation of cdc25C-cdc22 via ATM-Chk 1/2 stimulation. Therefore, we propose that Polyphyllin G might prove a lead molecule in the management of oral cancers and at the same time may prevent the growth of opportunistic microbes. Keywords: Anticancer; Antimicrobial; Cell cycle arrest; Metastasis; Oral cancer; Polyphyllin G.

In vivo:

Parasitology. 2013 Jul;140(8):952-8.

Identification of compounds from Paris polyphylla (ChongLou) active against Dactylogyrus intermedius.[Pubmed: 23552446]

The present study was designated to ascertain the anthelmintic activity of the rhizomes of Paris polyphylla and to isolate and characterize the active constituents.
METHODS AND RESULTS:
The methanol extract from rhizomes of P. polyphylla showed signiﬁcant anthelmintic activity against Dactylogyrus intermedius with the median effective concentration (EC50) 22.5 mg L(-1). Based on this finding, the methanol extract was fractionated by silica gel column chromatography in a bioassay-guided fractionation yielding 2 bioactive compounds, the structures of these compounds were elucidated as formosanin C and polyphyllin VII(Polyphyllin G). The in vivo tests revealed that formosanin C and polyphyllin VII were significantly effective against D. intermedius with EC50 values of 0.6 and 1.2 mg L(-1), respectively. The acute toxicities (LC50) of formosanin C and polyphyllin VII for grass carp were 2.8 and 2.9 mg L(-1), respectively.
CONCLUSIONS:
The overall results provide important information for the potential application of formosanin C and polyphyllin VII in the therapy of serious infection caused by D. intermedius.

Polyphyllin G Description

Source:	The rhizomes of Paris yunnanensis Franch.
Solvent:	DMSO, Pyridine, Methanol, Ethanol, etc.
Storage:	Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C). Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour. Need more advice on solubility, usage and handling? Please email to: service@chemfaces.com
After receiving:	The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.

Structure Identification:

Biomed Chromatogr. 2013 Mar;27(3):343-8

Simultaneous determination and pharmacokinetic study of polyphyllin I, polyphyllin II, polyphyllin VI and polyphyllin VII (Polyphyllin G) in beagle dog plasma after oral administration of Rhizoma Paridis extracts by LC-MS-MS.[Pubmed: 22903625]

For the first time, a rapid and specific LC-MS-MS method has been developed for the analysis of polyphyllin I, polyphyllin II, polyphyllin VI and polyphyllin VII(Polyphyllin G) in beagle dog plasma.
METHODS AND RESULTS:
The method was applied to study the pharmacokinetics of Rhizoma Paridis extracts containing polyphyllin I, polyphyllin II, polyphyllin VI and polyphyllin VII. The analysis was carried out on an Agilent Zorbax XDB-C(18) reversed-phase column (100 × 2.1 mm, 1.8 μm) by isocratic elution with acetonitrile and water (50:50, v/v). The flow rate was 0.25 mL/min. All analytes including internal standards were monitored by selected reaction monitoring with an electrospray ionization source. Linear responses were obtained for polyphyllin I, polyphyllin II, polyphyllin VI and polyphyllin VII ranging from 10 to 5000 ng/mL. The intra-and inter-day precisions (RSDs) were less than 6.66 and 9.15%. The extraction recovery ranged from 95.53 to 104.21% with RSD less than 8.69%. Stability studies showed that polyphyllin I, polyphyllin II, polyphyllin VI and polyphyllin VII were stable in preparation and analytical process.
CONCLUSIONS:
The validated method was successfully used to determine the concentration-time profiles of polyphyllin I, polyphyllin II, polyphyllin VI and polyphyllin VII.

Product Name	Polyphyllin G
Price:
CAS No.:	76296-75-8
Catalog No.:	CFN99955
Molecular Formula:	C₅₁H₈₄O₂₂
Molecular Weight:	1049.22 g/mol
Purity:	>=98%
Type of Compound:	Steroids
Physical Desc.:	Powder
Source:	The rhizomes of Paris yunnanensis Franch.
Solvent:	DMSO, Pyridine, Methanol, Ethanol, etc.
Download:	COA MSDS SDF Manual
Similar structural:	Comparison (Web) (SDF)
Guestbook:

Size /Price /Stock	10 mM * 100 uL in DMSO / Inquiry / In-stock 10 mM * 1 mL in DMSO / Inquiry / In-stock
Related Libraries	Anticancer Compound Library Steroids Compound Library p38MAPK Inhibitor Library JNK Inhibitor Library ERK Inhibitor Library Caspase Inhibitor Library Bcl-2/Bax Inhibitor Library Autophagy Inhibitor Library Antifection Inhibitor Library Akt Inhibitor Library

	1 mg	5 mg	10 mg	20 mg	25 mg
1 mM	0.9531 mL	4.7654 mL	9.5309 mL	19.0618 mL	23.8272 mL
5 mM	0.1906 mL	0.9531 mL	1.9062 mL	3.8124 mL	4.7654 mL
10 mM	0.0953 mL	0.4765 mL	0.9531 mL	1.9062 mL	2.3827 mL
50 mM	0.0191 mL	0.0953 mL	0.1906 mL	0.3812 mL	0.4765 mL
100 mM	0.0095 mL	0.0477 mL	0.0953 mL	0.1906 mL	0.2383 mL

CFN95345	Dioscoreside C		1061.2	C₅₂H₈₄O₂₂
CFN95346	Dioscoreside E	435321-73-6	1077.2	C₅₂H₈₄O₂₃
CFN90822	Terrestrosin K	193605-07-1	1079.2	C₅₁H₈₂O₂₄
CFN90705	Pseudoproto Pb	102100-46-9	1177.4	C₅₇H₉₂O₂₅
CFN90692	Officinalisinin I	57944-18-0	921.07	C₄₅H₇₆O₁₉
CFN99533	Anemarsaponin E	136565-73-6	934.03	C₄₆H₇₈O₁₉
CFN95321	(3beta,22alpha)-26-(beta-glucopyranosyloxy)-22-methoxyfurost-5-en-3-yl 2-O-(6-deoxy-alpha-mannopyranosyl)-beta-glucopyranosiduronic acid	107783-53-9	931.1	C₄₆H₇₄O₁₉
CFN99517	Protodioscin	55056-80-9	1049.21	C₅₁H₈₄O₂₂
CFN99585	Methyl protodioscin	54522-52-0	1063.23	C₅₂H₈₆O₂₂
CFN93008	Protodeltonin	94992-08-2	1065.21	C₅₁H₈₄O₂₃