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10-O-Methylprotosappanin B
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Product Name 10-O-Methylprotosappanin B
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CAS No.: 111830-77-4
Catalog No.: CFN96304
Molecular Formula: C17H18O6
Molecular Weight: 318.3 g/mol
Purity: >=98%
Type of Compound: Phenols
Physical Desc.: Powder
Source: The dried heartwoods of Caesalpinia sappan L.
Solvent: Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.
Download: COA    MSDS
Similar structural: Comparison (Web)  (SDF)
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Size /Price /Stock 10 mM * 1 mL in DMSO / Inquiry
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10 mM * 1 mL in DMSO / Inquiry / In-stock
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Biological Activity
Description: Standard reference
10-O-Methylprotosappanin B Description
Source: The dried heartwoods of Caesalpinia sappan L.
Solvent: Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.
Storage: Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).

Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

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After receiving: The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
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Recently, ChemFaces products have been cited in many studies from excellent and top scientific journals

Cell. 2018 Jan 11;172(1-2):249-261.e12.
doi: 10.1016/j.cell.2017.12.019.
IF=36.216(2019)

PMID: 29328914

Cell Metab. 2020 Mar 3;31(3):534-548.e5.
doi: 10.1016/j.cmet.2020.01.002.
IF=22.415(2019)

PMID: 32004475

Mol Cell. 2017 Nov 16;68(4):673-685.e6.
doi: 10.1016/j.molcel.2017.10.022.
IF=14.548(2019)

PMID: 29149595

ACS Nano. 2018 Apr 24;12(4): 3385-3396.
doi: 10.1021/acsnano.7b08969.
IF=13.903(2019)

PMID: 29553709

Nature Plants. 2016 Dec 22;3: 16206.
doi: 10.1038/nplants.2016.205.
IF=13.297(2019)

PMID: 28005066

Sci Adv. 2018 Oct 24;4(10): eaat6994.
doi: 10.1126/sciadv.aat6994.
IF=12.804(2019)

PMID: 30417089
Calculate Dilution Ratios(Only for Reference)
1 mg 5 mg 10 mg 20 mg 25 mg
1 mM 3.1417 mL 15.7085 mL 31.4169 mL 62.8338 mL 78.5423 mL
5 mM 0.6283 mL 3.1417 mL 6.2834 mL 12.5668 mL 15.7085 mL
10 mM 0.3142 mL 1.5708 mL 3.1417 mL 6.2834 mL 7.8542 mL
50 mM 0.0628 mL 0.3142 mL 0.6283 mL 1.2567 mL 1.5708 mL
100 mM 0.0314 mL 0.1571 mL 0.3142 mL 0.6283 mL 0.7854 mL
* Note: If you are in the process of experiment, it's need to make the dilution ratios of the samples. The dilution data of the sheet for your reference. Normally, it's can get a better solubility within lower of Concentrations.
Protocol
Structure Identification:
Chem. Pharm. Bull.,1987,35(9):3615-9.
Homoisoflavonoids and Related Compounds. V.1) A Novel Dibenzoxocin Derivative from Caesalpinia sappan L[Reference: WebLink]
We have been further studying the isolation, absolute stereochemistries and biosyntheses of the phenolic constituents of C. sappan and C. japonica.
METHODS AND RESULTS:
In the present paper,we report the isolation and structural elucidation of a novel dibenzoxocin derivative,10-O-Methylprotosappanin B(1), from Sappan Lignum.10-O-Methylprotosappanin B (1) was obtained by repeated silica gel and Sephadex LH-20 column chromatography, and preparative thin-layer chromatography (PTLC) of the remaining fractions after separation of the previously reported compounds.4) The isolation of the intact compound was so difficult that only a limited amount could be obtained. However, a satisfactory amount was obtained in the form of an isopropylidene derivative (6) from the methanolic extract separated after treatment with acetone in the presence of an acid catalyst, as described in the previous paper.4c) The remaining fractions after isolation of the isopropylidene derivatives of 3,4-dihydroxy homoisoflavans4c) were subjected to column chromatography on silica gel and Sephadex LH-20 to yield 6, together with the isopropylidene derivative of protosappanin B (7). On acid hydrolysis with 60% AcOH, compound 6 was readily transformed into 1.
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