• ChemFaces is a professional high-purity natural products manufacturer.
  • Product Intended Use
  • 1. Reference standards
  • 2. Pharmacological research
  • 3. Inhibitors
  • Home
  • Natural Products
  • Bioactive
  • Screening Libraries
  • Hot Products
  • Plant Catalog
  • Customer Support
  • Product Use Citation
  • About Us
  • Contact Us
  • Natural Products
    CAS No. 33570-04-6 Price $70 / 20mg
    Catalog No.CFN99789Purity>=98%
    Molecular Weight326.30Type of CompoundSesquiterpenoids
    FormulaC15H18O8Physical DescriptionWhite powder
    Download Manual    COA    MSDSSimilar structuralComparison (Web)
    How to Order
    Orders via your E-mail:

    1. Product number / Name / CAS No.
    2. Delivery address
    3. Ordering/billing address
    4. Contact information
    Sent to Email: info@chemfaces.com
    Contact Us
    Order & Inquiry & Tech Support

    Tel: (0086)-27-84237683
    Fax: (0086)-27-84254680
    E-mail: manager@chemfaces.com
    Address: No. 83, CheCheng Rd., WETDZ, Wuhan, Hubei 430056, PRC
    Delivery time
    Delivery & Payment method

    1. Usually delivery time: Next day delivery by 9:00 a.m. Order now

    2. We accept: Wire transfer & Credit card & Paypal & Western Union
    * Packaging according to customer requirements(5mg, 10mg, 20mg and more). We shipped via FedEx, DHL, UPS, EMS and others courier.
    Our products had been exported to the following research institutions and universities, And still growing.
  • University of Medicine and Pharm... (Romania)
  • University of Brasilia (Brazil)
  • University of Maryland School of... (USA)
  • Georgia Institute of Technology (USA)
  • Auburn University (USA)
  • Max-Planck-Insitut (Germany)
  • Seoul National University (Korea)
  • Nicolaus Copernicus Uniwersity (Poland)
  • Universite Libre de Bruxelles (Belgium)
  • University of Malaya (Malaysia)
  • University of Padjajaran (Indonesia)
  • More...
  • Package
    Featured Products
    Sophoraflavanone G

    Catalog No: CFN92005
    CAS No: 97938-30-2
    Price: $138/20mg
    Leachianone A

    Catalog No: CFN97560
    CAS No: 97938-31-3
    Price: $418/10mg
    Picroside III

    Catalog No: CFN99567
    CAS No: 64461-95-6
    Price: $228/20mg
    Ganoderol A

    Catalog No: CFN99065
    CAS No: 104700-97-2
    Price: $488/5mg

    Catalog No: CFN92616
    CAS No: 7727-79-9
    Price: $278/20mg
    Biological Activity
    Description: Bilobalide possesses anticonvulsant, insecticidal, and cardioprotective effects. Bilobalide exerts protective and trophic effects on neurons, the PI3K/Akt pathway may be involved in the protective effects of bilobalide; it also can protect PC12 cells from A beta 25-35-induced cytotoxicity, it dose-dependently attenuates the cytotoxic effect of A beta 25-35.
    Targets: PAFR | Beta Amyloid | PI3K | Akt | ERK | PKC | Serine
    In vitro:
    Acta Pharmacol Sin. 2000 Jan;21(1):75-9.
    Protective effects of bilobalide on amyloid beta-peptide 25-35-induced PC12 cell cytotoxicity.[Pubmed: 11263252]
    To study the effect of bilobalide[(-)-Bilobalide], a terpene extracted from the leaves of Ginkgo biloba, on beta-amyloid peptide fragment 25-35 (A beta 25-35)-induced PC12 cell cytotoxicity.
    3-[4,5-Dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide and lactate dehydrogenase assay were used to measure the viability of PC12 cells. Thiobarbituric acid-reactive substances were measured to determine lipid peroxidation of cells. Antioxidant enzymes in PC12 cells were detected. Treatment of PC12 cells with A beta 25-35 (100 mumol.L-1) for 24 h caused a great decrease in cell viability (P < 0.01 compared with control). Bilobalide[(-)-Bilobalide] 25-100 mumol.L-1 dose-dependently attenuated the cytotoxic effect of A beta 25-35. Bilobalide[(-)-Bilobalide] also inhibited A beta 25-35 (100 mumol.L-1)-induced elevation of lipid peroxidation and decline of antioxidant enzyme activities.
    Bilobalide[(-)-Bilobalide] protected PC12 cells from A beta 25-35-induced cytotoxicity.
    In vivo:
    Eur J Pharmacol. 1999 Feb 19;367(2-3):165-73.
    Effects of bilobalide on gamma-aminobutyric acid levels and glutamic acid decarboxylase in mouse brain.[Pubmed: 10078989]
    We have previously demonstrated that Bilobalide[(-)-Bilobalide] a constituent of the Ginkgo biloba extract, possesses anticonvulsant activity, and suggested that the mechanism of its anticonvulsant action involves modulation of y-aminobutyric acid (GABA)-related neuronal transmission.
    This study examined the effects of Bilobalide[(-)-Bilobalide] on the level of GABA and glutamate, the activity and the amount of glutamic acid decarboxylase (EC, and the function of GABA(A) receptors in the hippocampus, cerebral cortex and striatum of the mouse. GABA levels, glutamic acid decarboxylase activity, and the protein amount of 67 kDa glutamic acid decarboxylase in the hippocampus of mice treated withBilobalide[(-)-Bilobalide] (30 mg/kg, p.o., once a day for 4 days) were significantly higher than those in controls. However, there were no significant differences in glutamate levels or, the number and the dissociation constants of GABA(A) receptors in the hippocampus between control and bilobalide-treated mice.
    These results suggest that the anticonvulsant effect of bilobalide is due to elevation of GABA levels, possibly through potentiation of glutamic acid decarboxylase activity and enhancement of the protein amount of 67 kDa glutamic acid decarboxylase by bilobalideBilobalide[(-)-Bilobalide].
    (-)-Bilobalide Description
    Source: The leaves of Ginkgo biloba L.
    Solvent: Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.
    Storage: Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Need more advice on solubility, usage and handling? Please email to: service@chemfaces.com

    After receiving: The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
    Recent ChemFaces New Products and Compounds

    Catalog No: CFN98757
    CAS No: 482-39-3
    Price: $288/20mg

    Catalog No: CFN95009
    CAS No: 76844-70-7
    Price: $368/5mg
    Caohuoside E

    Catalog No: CFN95016
    CAS No: 174286-23-8
    Price: $388/5mg
    Monomethyl lithospermate

    Catalog No: CFN91005
    CAS No: 933054-33-2
    Price: $333/10mg

    Catalog No: CFN90448
    CAS No: 14941-08-3
    Price: $218/20mg

    Catalog No: CFN89301
    CAS No: 23202-85-9
    Price: $288/5mg

    Catalog No: CFN95079
    CAS No: 82474-97-3
    Price: $388/5mg
    4-O-Feruloylquinic acid

    Catalog No: CFN92392
    CAS No: 2613-86-7
    Price: $448/5mg
    Recently, ChemFaces products have been cited in many studies from excellent and top scientific journals

    Cell. 2018 Jan 11;172(1-2):249-261.e12.
    doi: 10.1016/j.cell.2017.12.019.

    PMID: 29328914

    Mol Cell. 2017 Nov 16;68(4):673-685.e6.
    doi: 10.1016/j.molcel.2017.10.022.

    PMID: 29149595

    Scientific Reports 2017 Dec 11;7(1):17332.
    doi: 10.1038/s41598-017-17427-6.

    PMID: 29230013

    Molecules. 2017 Oct 27;22(11). pii: E1829.
    doi: 10.3390/molecules22111829.

    PMID: 29077044

    J Cell Biochem. 2018 Feb;119(2):2231-2239.
    doi: 10.1002/jcb.26385.

    PMID: 28857247

    Phytomedicine. 2018 Feb 1;40:37-47.

    PMID: 29496173
    Calculate Dilution Ratios(Only for Reference)
    1 mg 5 mg 10 mg 20 mg 25 mg
    1 mM 3.0647 mL 15.3233 mL 30.6466 mL 61.2933 mL 76.6166 mL
    5 mM 0.6129 mL 3.0647 mL 6.1293 mL 12.2587 mL 15.3233 mL
    10 mM 0.3065 mL 1.5323 mL 3.0647 mL 6.1293 mL 7.6617 mL
    50 mM 0.0613 mL 0.3065 mL 0.6129 mL 1.2259 mL 1.5323 mL
    100 mM 0.0306 mL 0.1532 mL 0.3065 mL 0.6129 mL 0.7662 mL
    * Note: If you are in the process of experiment, it's need to make the dilution ratios of the samples. The dilution data of the sheet for your reference. Normally, it's can get a better solubility within lower of Concentrations.
    Kinase Assay:
    Apoptosis. 2010 Jun;15(6):715-27.
    Bilobalide prevents apoptosis through activation of the PI3K/Akt pathway in SH-SY5Y cells.[Pubmed: 20333467 ]
    Bilobalide[(-)-Bilobalide], a sesquiterpene trilactone constituent of Ginkgo biloba leaf extracts, has been proposed to exert protective and trophic effects on neurons. However, mechanisms underlying the protective effects of bilobalide [(-)-Bilobalide]remain unclear. Using human SH-SY5Y neuroblastoma cells and primary hippocampal neurons, this study investigated the neuroprotective effects of bilobalide[(-)-Bilobalide].
    We mimicked aging-associated neuronal impairments by applying external factors (beta amyloid protein (Abeta) 1-42, H(2)O(2) and serum deprivation) consequently inducing cell apoptosis. As markers for apoptosis, cell viability, DNA fragmentation, mitochondrial membrane potential and levels of cleaved caspase 3 were measured. We found that, bilobalide[(-)-Bilobalide] prevented Abeta 1-42-, H(2)O(2)- and serum deprivation-induced apoptosis. To better understand the neuroprotective effects of bilobalide[(-)-Bilobalide], we also tested the ability of bilobalide[(-)-Bilobalide] to modulate pro-survival signaling pathways such as protein kinase C (PKC), extracellular-regulated kinase 1/2 (ERK1/2) and phosphatidylinositol 3-kinase (PI3K)/Akt pathways. It was found that, bilobalide[(-)-Bilobalide] dose-dependently increased PI3K activity and levels of phosphorylated Akt (p-Akt Ser473 and Thr308), which could be maintained up to at least 2 h after bilobalide withdrawal in cells treated with or without Abeta 1-42, H(2)O(2) or serum-free medium. In addition, application of PI3K/Akt inhibitor LY294002 could abrogate both the protective effects of bilobalide[(-)-Bilobalide] against Abeta 1-42-, H(2)O(2)- and serum deprivation-induced apoptotic cell damage and bilobalide[(-)-Bilobalide]-induced increase in PI3K activity and levels of p-Akt (Ser473 and Thr308). In contrast, application of PKC inhibitor staurosporine (STS) did not affect the protective effects of bilobalide[(-)-Bilobalide]. Moreover, no change in levels of phosphorylated ERK1/2 (p-ERK1/2) was observed in bilobalide-treated cells.
    These results further suggested that the PI3K/Akt pathway might be involved in the protective effects of bilobalide[(-)-Bilobalide]. Since modern technology allows production of purified bilobalide[(-)-Bilobalide] with high bioavailability, bilobalide[(-)-Bilobalide] may be useful in developing therapy for diseases involving age-associated neurodegeneration.
    Cell Research:
    Biosci Rep. 2011 Oct 1; 31(Pt 5): 439–447.
    Anti-ischaemic effects of bilobalide on neonatal rat cardiomyocytes and the involvement of the platelet-activating factor receptor[Pubmed: 21391918]
    Terpene trilactones from Ginkgo biloba have been investigated extensively for their antioxidant and anti-ischaemic activities on the brain and the heart, but the mechanisms of these effects remain unclear.
    For the present study, a terpenoid constituent from G. biloba, bilobalide[(-)-Bilobalide], was screened for protective effects on the ischaemic heart and the involvement of the PAFR [PAF (platelet-activating factor) receptor] and the enzyme that degrades PAF, PAF-AH (PAF acetylhydrolase) during hypoxia. The PAF pathway is supposed to play a role in hypoxia and its regulation may prevent or alleviate MI (myocardial infarction). Cardiomyocytes from neonatal rat hearts were cultured and treated with different concentrations of bilobalide[(-)-Bilobalide] (500-0.5 ng/ml). After being subjected to a hypoxic environment, the cells' viability was evaluated and proteins as well as RNA were extracted for analysis by Western blotting and RT-PCR (reverse transcription PCR) respectively. With the MI model we tested for bilobalide[(-)-Bilobalide]'s cardioprotective effects and the involvement of PAFR and PAF-AH. Bilobalide ((-)-Bilobalide,5 ng/ml) significantly decreased the mortality of cells in a concentration-dependent way. mRNA expression of PAFR was up-regulated in hypoxic cells but in the groups treated with bilobalide[(-)-Bilobalide], its expression was down-regulated to the level of the normal control. In hypoxic tissue, PAFR protein expression was also up-regulated, but was reduced in the bilobalide ((-)-Bilobalide,10 mg/kg of body weight) treated group.
    Our results indicate that PAF and its receptor may be involved in the cellular response of cardiomyocytes to hypoxia and that bilobalide may interact with this receptor to exert its cardioprotective effects.
    Structure Identification:
    The Korean Society of Pesticide Science,2001,5(1):24-9.
    Insecticidal Activities of Bilobalide from Ginkgo biloba Leaves and its Derivatives.[Reference: WebLink]
    This study was conducted to investigate insecticidal activities of Ginkgo biloba (L.) leaves-derived bilobalide[(-)-Bilobalide] and its hydrolysis and oxidation products against adults of Nilaparavata lugens Stal.
    To find out active insecticidal moiety of bilobalide[(-)-Bilobalide], decomposed intermediates and derivatives of bilobalide[(-)-Bilobalide] were made by hydrolysis, oxidation, and acetylation. The structures of hydrolysis product by base and oxidation product by acid were identified as cyclopentenone analogues and trilactone sesquiterpene from dehydration of bilobalide[(-)-Bilobalide], respectively. Insecticidal activities of the decomposed intermediates and the derivatives of bilobalide[(-)-Bilobalide] decreased in the order of bilobalide[(-)-Bilobalide], monoacetate, ginkgolide C, oxidation product, diacetate, and hydrolysis product.
    Therefore, trilactone structure of bilobalide[(-)-Bilobalide] may be essential for its insecticidal activity.