| Structure Identification: |
| Separation Science\s&\stechnology, 2015 , 50 (15) :2360-2366. | | Separation and Purification of 15-Demethylplumieride, Cerberic Acid B, and Kaempferol-3-Rutinoside from Plumeria rubra ‘Acutifolia’ by High-Speed Counter-Current Chromatography[Reference: WebLink] |
METHODS AND RESULTS:
Three compounds, 15-demethylplumieride (I), Cerberic acid B (II), and kaempferol-3-rutinoside (III) were successfully separated from the flowers of Plumeria rubra ‘Acutifolia’ by high-speed counter-current chromatography (HSCCC) for the first time. The crude extract from Plumeria rubra ‘Acutifolia’ was treated with D101 macroporous resin and divided into two parts: fraction 1 (15% ethanol eluent) and fraction 2 (30% ethanol eluent). Two hundred and thirty milligrams of fraction 1 were separated by HSCCC with the solvent system of n -butanol-methanol-water (4:0.5:4, v/v/v), yielding 17 mg of I and 28 mg of II. One hundred and thirty-five milligrams of fraction 2 were separated by HSCCC with the solvent system of ethyl acetate- n -butanol-water (4.4:0.6:5, v/v/v), yielding 7 mg of III. The purities of the three compounds were determined by HPLC-DAD as 96.04%, 98.38%, and 97.97%, respectively.
CONCLUSIONS:
Their structures were identified by UV, MS, and NMR. The established methods were simple, fast, and convenient, which can be applied to the preparation of reference substances for bioactivity, quality control, fingerprint analysis, and other research of Plumeria rubra ‘Acutifolia’. |
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