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    Cinobufaginol
    Cinobufaginol
    Information
    CAS No. 6691-83-4 Price $333 / 5mg
    Catalog No.CFN91051Purity>=98%
    Molecular Weight458.55Type of CompoundSteroids
    FormulaC26H34O7Physical DescriptionPowder
    Download     COA    MSDSSimilar structuralComparison (Web)
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    Cinobufaginol Description
    Source: The glandular body of Bufo bufo gargarizans Cantor.
    Biological Activity or Inhibitors:
    Solvent: Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.
    Storage: Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Need more advice on solubility, usage and handling? Please email to: service@chemfaces.com

    After receiving: The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
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    Recently, ChemFaces products have been cited in many studies from excellent and top scientific journals

    Cell. 2018 Jan 11;172(1-2):249-261.e12.
    doi: 10.1016/j.cell.2017.12.019.

    PMID: 29328914

    Mol Cell. 2017 Nov 16;68(4):673-685.e6.
    doi: 10.1016/j.molcel.2017.10.022.

    PMID: 29149595

    Scientific Reports 2017 Dec 11;7(1):17332.
    doi: 10.1038/s41598-017-17427-6.

    PMID: 29230013

    Molecules. 2017 Oct 27;22(11). pii: E1829.
    doi: 10.3390/molecules22111829.

    PMID: 29077044

    J Cell Biochem. 2018 Feb;119(2):2231-2239.
    doi: 10.1002/jcb.26385.

    PMID: 28857247

    Phytomedicine. 2018 Feb 1;40:37-47.
    doi:10.1016/j.phymed.2017.12.030

    PMID: 29496173
    Calculate Dilution Ratios(Only for Reference)
    1 mg 5 mg 10 mg 20 mg 25 mg
    1 mM 2.1808 mL 10.9039 mL 21.8079 mL 43.6157 mL 54.5197 mL
    5 mM 0.4362 mL 2.1808 mL 4.3616 mL 8.7231 mL 10.9039 mL
    10 mM 0.2181 mL 1.0904 mL 2.1808 mL 4.3616 mL 5.452 mL
    50 mM 0.0436 mL 0.2181 mL 0.4362 mL 0.8723 mL 1.0904 mL
    100 mM 0.0218 mL 0.109 mL 0.2181 mL 0.4362 mL 0.5452 mL
    * Note: If you are in the process of experiment, it's need to make the dilution ratios of the samples. The dilution data of the sheet for your reference. Normally, it's can get a better solubility within lower of Concentrations.
    Cinobufaginol References Information
    Citation [1]

    Chemical Research in Chinese Universities, 2009, 25(6):801-806.

    Simultaneous Determination of Bufadienolides and Qualitative Evaluation for Venenum Bufonis by High Performance Liquid Chromatography[Reference: WebLink]
    A high performance liquid chromatographic method was used for the simultaneous identification and qua-litative evaluation of 12 bufadienolides(resibufogenin, cinobufagin, Cinobufaginol, arenobufagin, bufalin, bufotalin, gamabufotalin, cinobufotalin, Ψ-bufaranogin, desacetylcinobufagin, telocinobufagin and resibufogenol) in Venenum Bufonis. The chromatographic separation was performed on a Dikma C 18 analytical column via gradient elution with an aqueous solution of acetonitrile and 0.3% acetic acid at a flow rate of 0.8 mL/min. The method was validated to be acceptable in consideration of linearity(r 2 > 0.9992) and recovery(ranged from 98.9% to 102.0%). The limits of de-tection of the bufadienolides were from 0.48 ng for bufalin to 6.00 ng for cinobufotalin. The intra-day and inter-day precisions of the method were evaluated and were less than 3.0%. The method was successfully used to analyze 19 batches of Venenum Bufonis, and the similarity values between batches were calculated by Similarity Evaluation System for Chromatographic Fingerprint of TCM(Version 2004A, Chinese Pharmacopoeia Committee, Beijing). The results show that the contents of bufadienolides in the medicine and the similarity values based on these bufadieno-lides varied significantly from batch to batch. This proposed method could be utilized to qualify and control Venenum Bufonis to ensure its safety and efficacy in application.