|Source:||The rhizomes of Paris yunnanensis Franch.|
|Solvent:||DMSO, Pyridine, Methanol, Ethanol, etc.|
|Storage:||Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please email to: email@example.com
|After receiving:||The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.|
|1 mg||5 mg||10 mg||20 mg||25 mg|
|1 mM||0.8366 mL||4.1829 mL||8.3658 mL||16.7316 mL||20.9146 mL|
|5 mM||0.1673 mL||0.8366 mL||1.6732 mL||3.3463 mL||4.1829 mL|
|10 mM||0.0837 mL||0.4183 mL||0.8366 mL||1.6732 mL||2.0915 mL|
|50 mM||0.0167 mL||0.0837 mL||0.1673 mL||0.3346 mL||0.4183 mL|
|100 mM||0.0084 mL||0.0418 mL||0.0837 mL||0.1673 mL||0.2091 mL|
《Chinese Journal of Natural Medicines》 2009-05
|Biotransformation of Dichotomin by Pectinex BE XXL[Reference: WebLink]|
|AIM:To study of structural modification in steroidal saponin by biotransformation technology.METHODS:Structural modification was carried out of glycosyl residues of Dichotomin at C-3 position by Pectinex BE XXL(PBX).Cytotoxicity against HL-60 cells of all products was investigated.RESULTS:The PBX could hydrolyze the glycosyl residues of Dichotomin one-by-one to produce nine transformed compounds.The transformed product(mixture) and four secondary spirostanosides among them exhibited cytotoxicity.Compounds P5,P6 and P7 with α-1,2 end-rhamnosyl residues showed a potential cytotoxic effect on HL-60 cells;P8 with α-1,4 end-rhamnosyl residue had a weak effect on HL-60 cells.Dichotomin,secondary furostanosides and aglycone were not cytotoxic.CONCLUSIONS:PBX has various hydrolyzation to the glycosidic bonds of Dichotomin.Nine obtained transformed compounds widen steroidal saponin library.Meanwhile,screening activity affords data for further pharmacological study.|