|Description:|| 1. Diosbulbin D shows direct toxic effect on hepatocytes, the mechanism could be associated with oxidative stress.|
|Source:||The roots of Dioscorea bulbifera|
|Solvent:||Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.|
|Storage:||Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please email to: firstname.lastname@example.org
|After receiving:||The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.|
|1 mg||5 mg||10 mg||20 mg||25 mg|
|1 mM||2.9036 mL||14.518 mL||29.036 mL||58.072 mL||72.59 mL|
|5 mM||0.5807 mL||2.9036 mL||5.8072 mL||11.6144 mL||14.518 mL|
|10 mM||0.2904 mL||1.4518 mL||2.9036 mL||5.8072 mL||7.259 mL|
|50 mM||0.0581 mL||0.2904 mL||0.5807 mL||1.1614 mL||1.4518 mL|
|100 mM||0.029 mL||0.1452 mL||0.2904 mL||0.5807 mL||0.7259 mL|
|Diosbulbin d and 8-epidiosbulbin e acetate, norclerodane diterpenoids from dioscorea bulbifera tubers.[Reference: WebLink]|
|From tubers of Dioscorea buibifera L. var sativa a new 18-norclerodane diterpenoid, 8-epidiosbulbin E acetate, has been isolated, besides the previously known norditerpenoid, Diosbulbin D, and its structure established by spectroscopic means.|
Chinese Journal of Natural Medicines, 2011, 9(4):280-5.
|Toxicity of a Diterpene Lactone Isolated from Dioscorea bulbifera on Hepatocytes[Reference: WebLink]|
|Using the MTT assay to test the effect of Diosbulbin D on hepatocytes.Selected intracellular enzymes release levels by diosbulbin-D were determined to further validate the toxic effect on hepatocytes.Furthermore,the level of intracellular glutathione and the induction of reactive oxygen species(ROS) by diosbulbin-D were measured by fluorometric and flow cytometry methods.RESULTS:Diosbulbin-D showed toxic effect on hepatocytes and a significant increase in intracellular release levels of LDH and the liver enzymes,alanine aminotransferase and aspartate aminotransferase confirmed diosbulbin-D's toxic effect.ROS increase was related to the decrease in level of GSH.Cell pretreated NAC almost completely blocked the ROS fluorescence and recovered the cell growth inhibition induced by diosbulbin-D.CONCLUSION:Diosbulbin-D shows direct toxic effect on hepatocytes.The mechanism could be associated with oxidative stress.|