Science | Nature | Cell | View More
Natural Products
Ergosterol
ChemFaces products have been cited in many studies from excellent and top scientific journals
Product Name Ergosterol
Price: $30 / 20mg
CAS No.: 57-87-4
Catalog No.: CFN98980
Molecular Formula: C28H44O
Molecular Weight: 396.7 g/mol
Purity: >=98%
Type of Compound: Steroids
Physical Desc.: Powder
Source: The endophytic fungus of Ocimum basilicum.
Solvent: Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.
Download: COA    MSDS    SDF    Manual
Similar structural: Comparison (Web)  (SDF)
Guestbook:
Contact Us
Order & Inquiry & Tech Support

Tel: (0086)-27-84237683
Tech: service@chemfaces.com
Order: manager@chemfaces.com
Address: 176, CheCheng Eest Rd., WETDZ, Wuhan, Hubei 430056, PRC
How to Order
Orders via your E-mail:

1. Product number / Name / CAS No.
2. Delivery address
3. Ordering/billing address
4. Contact information
Order: manager@chemfaces.com
Delivery time
Delivery & Payment method

1. Usually delivery time: Next day delivery by 9:00 a.m. Order now

2. We accept: Wire transfer & Credit card & Paypal
Citing Use of our Products
* Packaging according to customer requirements(5mg, 10mg, 20mg and more). We shipped via FedEx, DHL, UPS, EMS and others courier.
According to end customer requirements, ChemFaces provide solvent format. This solvent format of product intended use: Signaling Inhibitors, Biological activities or Pharmacological activities.
Size /Price /Stock 10 mM * 1 mL in DMSO / $10.8 / In-stock
Other Packaging *Packaging according to customer requirements(100uL/well, 200uL/well and more), and Container use Storage Tube With Screw Cap
Our products had been exported to the following research institutions and universities, And still growing.
  • Cornell University (USA)
  • Melbourne University (Australia)
  • University of Queensland (Australia)
  • Almansora University (Egypt)
  • Universite Libre de Bruxelles (Belgium)
  • Korea Institute of Oriental Med... (Korea)
  • University of Fribourg (Switzerland)
  • Charles Sturt University (Denmark)
  • Mendel University in Brno (Czech Republic)
  • University of Eastern Finland (Finland)
  • Max-Planck-Insitut (Germany)
  • More...
Package
Featured Products
Erianin

Catalog No: CFN90204
CAS No: 95041-90-0
Price: $118/20mg
Phillygenin

Catalog No: CFN90511
CAS No: 487-39-8
Price: $118/20mg
Isosinensetin

Catalog No: CFN90806
CAS No: 17290-70-9
Price: $218/20mg
Esculentoside C

Catalog No: CFN98164
CAS No: 65931-92-2
Price: $368/5mg
Ginsenoside F5

Catalog No: CFN95034
CAS No: 189513-26-6
Price: $298/20mg
Platycodin D

Catalog No: CFN98134
CAS No: 58479-68-8
Price: $70/20mg
Ononin

Catalog No: CFN99136
CAS No: 486-62-4
Price: $50/20mg
Ginsenoside Ra2

Catalog No: CFN93293
CAS No: 83459-42-1
Price: $218/5mg
Cannabidiolic acid

Catalog No: CFN99356
CAS No: 1244-58-2
Price: $413/5mg
Ganoderic acid B

Catalog No: CFN92052
CAS No: 81907-61-1
Price: $288/20mg
Related Screening Libraries
Size /Price /Stock 10 mM * 100 uL in DMSO / Inquiry / In-stock
10 mM * 1 mL in DMSO / Inquiry / In-stock
Related Libraries
Biological Activity
Description: Ergosterol is the primary sterol found in fungi, with antioxidative, anti-proliferative, and anti-inflammatory effects, ergosterol peroxide has the potential to be developed as a therapeutic agent to prevent renal fibrosis. Ergosterol can promote pheromone signaling and plasma membrane fusion in mating yeast, the critical requirement for ergosterol in V-ATPase function may underlie the antifungal activity of azoles.
Targets: TGF-β/Smad | ERK | p38MAPK | JNK | ATPase | Calcium Channel | Antifection
In vitro:
Phytomedicine. 2014 Feb 15;21(3):372-8.
Ergosterol peroxide from Cordyceps cicadae ameliorates TGF-β1-induced activation of kidney fibroblasts.[Pubmed: 24095053]
Chronic kidney disease is a growing public health problem with an urgent need for new pharmacological agents. Ergosterol peroxide (EP) is the major sterol produced by Cordyceps cicadae Shing (C. cicadae), a widely used traditional Chinese medicine. C. cicadae has been used to treat many kinds of diseases and has a potential benefit on renoprotection. This study aimed to investigate the anti-fibrotic effects of EP as well as the underlying mechanisms.
METHODS AND RESULTS:
A normal rat kidney fibroblast cell line (NRK-49F) was stimulated to undergo fibroblast activation by transforming growth factor-β1 (TGF-β1) and EP treatment was applied to explore its potential anti-fibrotic effects. Cell proliferation was investigated using MTT analysis. Fibrosis-associated protein expression was analyzed using immunohistochemistry and/or Western blotting. EP treatment attenuated TGF-β1-induced renal fibroblast proliferation, expression of cytoskeleton protein and CTGF, as well as ECM production. Additionally, EP blocked TGF-β1-stimulated phosphorylation of ERK1/2, p38 and JNK pathway. Moreover, the TGF-β1-induced expression of fibronectin was attenuated by either inhibition of MAPKs or by EP treatment.
CONCLUSIONS:
In conclusion, our findings demonstrate that EP is able to suppress TGF-β1-induced fibroblasts activation in NRK-49F. This new information provides a line of theoretical evidence supporting the use of C. cicadae in the intervention of kidney disease and suggests that EP has the potential to be developed as a therapeutic agent to prevent renal fibrosis.
BMC Complement Altern Med. 2013 Sep 17;13:229.
Trans-chalcone and quercetin down-regulate fatty acid synthase gene expression and reduce ergosterol content in the human pathogenic dermatophyte Trichophyton rubrum.[Pubmed: 24044691]
Fatty acid synthase (FAS) is a promising antifungal target due to its marked structural differences between fungal and mammalian cells. The aim of this study was to evaluate the antifungal activity of flavonoids described in the scientific literature as FAS inhibitors (quercetin, trans-chalcone, ellagic acid, luteolin, galangin, and genistein) against the dermatophyte Trichophyton rubrum and their effects on fatty acid and Ergosterol synthesis.
METHODS AND RESULTS:
The antifungal activity of the natural products was tested by the microdilution assay for determination of the minimum inhibitory concentration (MIC). The effect of the compounds on the cell membrane was evaluated using a protoplast regeneration assay. Ergosterol content was quantified by spectrophotometry. Inhibition of FAS by flavonoids was evaluated by an enzymatic assay to determine IC50 values. Quantitative RT-PCR was used to measure transcription levels of the FAS1 and ERG6 genes involved in fatty acid and Ergosterol biosynthesis, respectively, during exposure of T. rubrum to the flavonoids tested. The flavonoids quercetin and trans-chalcone were effective against T. rubrum, with MICs of 125 and 7.5 μg/mL for the wild-type strain (MYA3108) and of 63 and 1.9 μg/mL for the ABC transporter mutant strain (ΔTruMDR2), respectively. The MICs of the fluconazole and cerulenin controls were 63 and 125 μg/mL for the wild-type strain and 30 and 15 μg/mL for the mutant strain, respectively. Quercetin and trans-chalcone also reduced Ergosterol content in the two strains, indicating that interference with fatty acid and Ergosterol synthesis caused cell membrane disruption. The MIC of quercetin reduced the number of regenerated protoplasts by 30.26% (wild-type strain) and by 91.66% (mutant strain). Half the MIC (0.5 MIC) of quercetin did not reduce the number of regenerated wild-type fungal colonies, but caused a 36.19% reduction in the number of mutant strain protoplasts. In contrast, the MIC and 0.5 MIC of trans-chalcone and cerulenin drastically reduced protoplast regeneration in the two strains. The FAS1 gene was repressed in the presence of MICs of quercetin, trans-chalcone, fluconazole and cerulenin. The ERG6 gene was induced in the presence of MICs of fluconazole and cerulenin and was repressed in the presence of MICs of trans-chalcone and quercetin. Trans-chalcone and quercetin inhibited the enzymatic activity of FAS, with IC50 values of 68.23 and 17.1 μg/mL, respectively.
CONCLUSIONS:
Trans-chalcone and quercetin showed antifungal activity against T. rubrum, reducing Ergosterol levels and modulating the expression of FAS1 and ERG6.
J Microbiol Methods. 2004 Nov;59(2):253-62.
Ergosterol as a measure of living fungal biomass: persistence in environmental samples after fungal death.[Pubmed: 15369861 ]
The membrane lipid Ergosterol is found almost exclusively in fungi, and is frequently used by environmental microbiologists as an indicator of living fungal biomass, based on the assumption that Ergosterol is labile, and therefore rapidly degraded after the death of fungal hyphae.
METHODS AND RESULTS:
We studied the degradation of Ergosterol in environmental samples without living fungi. Under the conditions used in this study, Ergosterol was very stable both when added as a pure compound and when associated with dead fungi. The decrease of Ergosterol was at most 34% during 2 months when protected from sunlight. Presence of a natural bacterial assemblage did not enhance degradation over this time period, as compared to sterile controls. However, photochemical degradation was significant, and led to a 43% decrease of in Ergosterol content during 24 h.
CONCLUSIONS:
These results suggest that Ergosterol should be used cautiously as a biomarker for living fungi.
Biosci Biotechnol Biochem . 2018 Oct;82(10):1803-1811.
Ergosterol and its derivatives from Grifola frondosa inhibit antigen-induced degranulation of RBL-2H3 cells by suppressing the aggregation of high affinity IgE receptors[Pubmed: 29968517]
Abstract Grifola frondosa is an edible mushroom consumed as a health food and/or traditional medicine in Asia. However, the anti-allergic effects of G. frondosa are not yet understood. In this study, we demonstrated the effects of G. frondosa extract (GFE) on IgE-mediated allergic responses, using antigen-stimulated RBL-2H3 cells. Three active compounds: Ergosterol, 6β-methoxyergosta-7,22-dien-3β,5α-diol (MEDD), and 6-oxoergosta-7,22-dien-3β-ol (6-OXO) were isolated from GFE and shown to inhibit the antigen-induced release of β-hexosaminidase and histamine. Among the three active components, we focused on Ergosterol because of its high content in GFE. Ergosterol inhibited the aggregation of high-affinity IgE receptor (FcεRI), which is the first step in the activation of mast cells and antigen-induced tyrosine phosphorylation. Furthermore, Ergosterol suppressed antigen-increased IL-4 and TNF-α mRNA. Taken together, our findings suggest that G. frondosa, including Ergosterol and its derivatives as active components, has the potential to be a novel functional food that prevents type I allergies. Keywords: Anti-allergic activity; functional food; mast cells; mushroom; type I allergy.
In vivo:
PLoS Pathog. 2010 Jun 3;6(6):e1000939.
Requirement for ergosterol in V-ATPase function underlies antifungal activity of azole drugs.[Pubmed: 20532216]
Ergosterol is an important constituent of fungal membranes. Azoles inhibit Ergosterol biosynthesis, although the cellular basis for their antifungal activity is not understood. We used multiple approaches to demonstrate a critical requirement for Ergosterol in vacuolar H(+)-ATPase function, which is known to be essential for fungal virulence.
METHODS AND RESULTS:
Ergosterol biosynthesis mutants of S. cerevisiae failed to acidify the vacuole and exhibited multiple vma(-) phenotypes. Extraction of Ergosterol from vacuolar membranes also inactivated V-ATPase without disrupting membrane association of its subdomains. In both S. cerevisiae and the fungal pathogen C. albicans, fluconazole impaired vacuolar acidification, whereas concomitant Ergosterol feeding restored V-ATPase function and cell growth. Furthermore, fluconazole exacerbated cytosolic Ca(2+) and H(+) surges triggered by the antimicrobial agent amiodarone, and impaired Ca(2+) sequestration in purified vacuolar vesicles. These findings provide a mechanistic basis for the synergy between azoles and amiodarone observed in vitro. Moreover, we show the clinical potential of this synergy in treatment of systemic fungal infections using a murine model of Candidiasis.
CONCLUSIONS:
In summary, we demonstrate a new regulatory component in fungal V-ATPase function, a novel role for Ergosterol in vacuolar ion homeostasis, a plausible cellular mechanism for azole toxicity in fungi, and preliminary in vivo evidence for synergism between two antifungal agents. New insights into the cellular basis of azole toxicity in fungi may broaden therapeutic regimens for patient populations afflicted with systemic fungal infections.
Ergosterol Description
Source: The endophytic fungus of Ocimum basilicum.
Solvent: Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.
Storage: Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).

Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

Need more advice on solubility, usage and handling? Please email to: service@chemfaces.com

After receiving: The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
ChemFaces New Products and Compounds
N-trans-caffeoyltyramine

Catalog No: CFN95265
CAS No: 103188-48-3
Price: $318/5mg
4'-Hydroxy-5,7,3'-trimethoxyflavon...

Catalog No: CFN95398
CAS No: 1239-68-5
Price: $318/5mg
Otobaphenol

Catalog No: CFN95476
CAS No: 10240-16-1
Price: $318/10mg
Citrusin B

Catalog No: CFN95178
CAS No: 105279-10-5
Price: $388/5mg
Rhamnocitrin 3-galactoside

Catalog No: CFN95474
CAS No: 99878-05-4
Price: $318/10mg
Oxyphyllol B

Catalog No: CFN95575
CAS No: 226546-99-2
Price: $/mg
2-Methoxy-1,4-naphthoquinone

Catalog No: CFN95269
CAS No: 2348-82-5
Price: $70/20mg
1-Phenyl-2-propanol

Catalog No: CFN95129
CAS No: 14898-87-4
Price: $30/20mg
Recently, ChemFaces products have been cited in many studies from excellent and top scientific journals

Cell. 2018 Jan 11;172(1-2):249-261.e12.
doi: 10.1016/j.cell.2017.12.019.
IF=36.216(2019)

PMID: 29328914

Cell Metab. 2020 Mar 3;31(3):534-548.e5.
doi: 10.1016/j.cmet.2020.01.002.
IF=22.415(2019)

PMID: 32004475

Mol Cell. 2017 Nov 16;68(4):673-685.e6.
doi: 10.1016/j.molcel.2017.10.022.
IF=14.548(2019)

PMID: 29149595

ACS Nano. 2018 Apr 24;12(4): 3385-3396.
doi: 10.1021/acsnano.7b08969.
IF=13.903(2019)

PMID: 29553709

Nature Plants. 2016 Dec 22;3: 16206.
doi: 10.1038/nplants.2016.205.
IF=13.297(2019)

PMID: 28005066

Sci Adv. 2018 Oct 24;4(10): eaat6994.
doi: 10.1126/sciadv.aat6994.
IF=12.804(2019)

PMID: 30417089
Calculate Dilution Ratios(Only for Reference)
1 mg 5 mg 10 mg 20 mg 25 mg
1 mM 2.5208 mL 12.604 mL 25.208 mL 50.4159 mL 63.0199 mL
5 mM 0.5042 mL 2.5208 mL 5.0416 mL 10.0832 mL 12.604 mL
10 mM 0.2521 mL 1.2604 mL 2.5208 mL 5.0416 mL 6.302 mL
50 mM 0.0504 mL 0.2521 mL 0.5042 mL 1.0083 mL 1.2604 mL
100 mM 0.0252 mL 0.126 mL 0.2521 mL 0.5042 mL 0.6302 mL
* Note: If you are in the process of experiment, it's need to make the dilution ratios of the samples. The dilution data of the sheet for your reference. Normally, it's can get a better solubility within lower of Concentrations.
Protocol
Cell Research:
J Cell Biol. 2008 Feb 25;180(4):813-26.
Ergosterol promotes pheromone signaling and plasma membrane fusion in mating yeast.[Pubmed: 18299351]
Ergosterol depletion independently inhibits two aspects of yeast mating: pheromone signaling and plasma membrane fusion. In signaling, Ergosterol participates in the recruitment of Ste5 to a polarized site on the plasma membrane. Ergosterol is thought to form microdomains within the membrane by interacting with the long acyl chains of sphingolipids.
METHODS AND RESULTS:
We find that although sphingolipid-free Ergosterol is concentrated at sites of cell-cell contact, transmission of the pheromone signal at contact sites depends on a balanced ratio of Ergosterol to sphingolipids. If a mating pair forms between Ergosterol-depleted cells despite the attenuated pheromone response, the subsequent process of membrane fusion is retarded. Prm1 also participates in membrane fusion. However, Ergosterol and Prm1 have independent functions and only prm1 mutant mating pairs are susceptible to contact-dependent lysis. In contrast to signaling, plasma membrane fusion is relatively insensitive to sphingolipid depletion.
CONCLUSIONS:
Thus, the sphingolipid-free pool of Ergosterol promotes plasma membrane fusion.
Erianin

Catalog No: CFN90204
CAS No: 95041-90-0
Price: $118/20mg
Phillygenin

Catalog No: CFN90511
CAS No: 487-39-8
Price: $118/20mg
Isosinensetin

Catalog No: CFN90806
CAS No: 17290-70-9
Price: $218/20mg
Esculentoside C

Catalog No: CFN98164
CAS No: 65931-92-2
Price: $368/5mg
Ginsenoside F5

Catalog No: CFN95034
CAS No: 189513-26-6
Price: $298/20mg
Platycodin D

Catalog No: CFN98134
CAS No: 58479-68-8
Price: $70/20mg
Ononin

Catalog No: CFN99136
CAS No: 486-62-4
Price: $50/20mg
Ginsenoside Ra2

Catalog No: CFN93293
CAS No: 83459-42-1
Price: $218/5mg
Cannabidiolic acid

Catalog No: CFN99356
CAS No: 1244-58-2
Price: $413/5mg
Ganoderic acid B

Catalog No: CFN92052
CAS No: 81907-61-1
Price: $288/20mg
Tags: buy Ergosterol | Ergosterol supplier | purchase Ergosterol | Ergosterol cost | Ergosterol manufacturer | order Ergosterol | Ergosterol distributor