| Description: |
Falcarindiol has antimutagenic, neuroprotective, antifungal, anti-bacterial, and anticancer activities, it could be potentially used in food manufactures and cosmetology as preservative agents and biopesticides, or in medicine as new antibiotics. Falcarindiol has protective effects against CCl(4) toxicity, in part, the effects might be explained by anti-lipid peroxidation activity associated with the induction of the GSTs including GSTA4. |
| Targets: |
GSK-3 | NO | NOS | NADPH-oxidase | P450 (e.g. CYP17) |
| In vitro: |
| Cell Death Dis. 2012 Aug 23;3:e376. | | The antitumor natural compound falcarindiol promotes cancer cell death by inducing endoplasmic reticulum stress.[Pubmed: 22914324 ] | Falcarindiol (FAD) is a natural polyyne with various beneficial biological activities.
METHODS AND RESULTS:
We show here that FAD preferentially kills colon cancer cells but not normal colon epithelial cells. Furthermore, FAD inhibits tumor growth in a xenograft tumor model and exhibits strong synergistic killing of cancer cells with 5-fluorouracil, an approved cancer chemotherapeutic drug. We demonstrate that FAD-induced cell death is mediated by induction of endoplasmic reticulum (ER) stress and activation of the unfolded protein response (UPR). Decreasing the level of ER stress, either by overexpressing the ER chaperone protein glucose-regulated protein 78 (GRP78) or by knockout of components of the UPR pathway, reduces FAD-induced apoptosis. In contrast, increasing the level of ER stress by knocking down GRP78 potentiates FAD-induced apoptosis. Finally, FAD-induced ER stress and apoptosis is correlated with the accumulation of ubiquitinated proteins, suggesting that FAD functions at least in part by interfering with proteasome function, leading to the accumulation of unfolded protein and induction of ER stress. Consistent with this, inhibition of protein synthesis by cycloheximide significantly decreases the accumulation of ubiquitinated proteins and blocks FAD-induced ER stress and cell death.
CONCLUSIONS:
Taken together, our study shows that FAD is a potential new anticancer agent that exerts its activity through inducing ER stress and apoptosis. | | J. Agr. Food Chem., 1996, 44(11): 3444-8. | | Antimutagenic activity of falcarindiol from Peucedanum praeruptorum.[Reference: WebLink] | A methanol extract from Peucedanum praeruptorum showed a suppressive effect on umu gene expression of the SOS response in Salmonella typhimurium TA1535/pSK1002 against the mutagen 2-(2-furyl)-3-(5-nitro-2-furyl)acrylamide (furylfuramide).
METHODS AND RESULTS:
The methanol extract from P. praeruptorum was re-extracted with hexane, dichloromethane, n-butanol, and water, respectively. A suppressive compound in the hexane extract fraction was isolated by SiO2 column chromatography and identified as Falcarindiol by EI-MS, IR, and 1H and 13C NMR spectroscopy. Falcarindiol exhibited an inhibition of the SOS-inducing activity of furylfuramide in the umu test. Gene expression was suppressed 75% at less than 0.15 μmol/mL, and the ID50 value was 0.10 μmol/mL. The diacetate compound of Falcarindiol did not show any suppressive effect on the SOS induction of furylfuramide. Falcarindiol was also assayed with the mutagen 3-amino-1,4-dimethyl-5H-pyrido[4,3-b]indole (Trp-P-1), which requires liver-metabolizing enzymes, and showed a suppressive effect similar to that with furylfuramide. The Falcarindiol ID50 value versus Trp-P-1 was 0.096 μmol/mL.
CONCLUSIONS:
The antimutagenic activities of Falcarindiol and Falcarindiol diacetate against furylfuramide and Trp-P-1 were tested by an Ames test using S. typhimurium TA100, which indicated that Falcarindiol suppressed the mutagenicity of furylfuramide and Trp-P-1 and Falcarindiol diacetate suppressed the mutagenicity of Trp-P-1. | | Planta Med., 1988, 54(1):36-7. | | Isolation of the Antifungal Compounds Falcarindiol and Sarisan from Heteromorpha trifoliata[Reference: WebLink] | | Leaves of Heteromorpha trifoliata (Umbelliferae) furnished the antifungal compounds Falcarindiol and sarisan after flash and low-pressure liquid chromatographies.
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| In vivo: |
| J Agric Food Chem. 2013 Aug 7;61(31):7515-21. | | Inhibition of glycogen synthase kinase-3β by falcarindiol isolated from Japanese Parsley (Oenanthe javanica).[Pubmed: 23895038] |
METHODS AND RESULTS:
A new biological activity of Falcarindiol isolated from Japanese parsley (Oenanthe javanica) using the mutant yeast YNS17 strain (zds1Δ erg3Δ pdr1Δ pdr3Δ) was discovered as an inhibitor of glycogen synthase kinase-3β (GSK-3β). Falcarindiol inhibited GSK-3β in an ATP noncompetitive manner with a Ki value of 86.9 μM using a human enzyme and luminescent kinase assay platform. Falcarindiol also both suppressed gene expression of glucose-6-phosphatase (G6Pase) in rat hepatoma H4IIE cells and protected mouse neuroblastoma HT22 cells from glutamate-induced oxidative cell death at 10 μM. During an oral glucose tolerance test (OGTT), the blood glucose level was significantly decreased in the rats treated with oral administration of O. javanica extract containing Falcarindiol (15 mg/kg).
CONCLUSIONS:
These findings indicate that Japanese parsley could be a useful food ingredient against type-2 diabetes and Alzheimer's disease. | | Biol Pharm Bull. 2011;34(3):371-8. | | Dietary diacetylene falcarindiol induces phase 2 drug-metabolizing enzymes and blocks carbon tetrachloride-induced hepatotoxicity in mice through suppression of lipid peroxidation.[Pubmed: 21372387] | Falcarindiol is a diacetylenic natural product containing unique carbon-carbon triple bonds.
METHODS AND RESULTS:
Mice were orally administrated Falcarindiol (100 mg/kg), and drug-metabolizing and antioxidant enzymes were monitored in several tissues of mice. Treatment with Falcarindiol was found to increase glutathione S-transferase (GST) and NAD(P)H: quinone oxidoreductase 1 activities in liver, small intestine, kidney, and lung. No changes were observed in cytochrome P450 (CYP) 1A known to activate procarcinogens. Western blot analysis revealed that various GST subunits including GSTA4, which plays an important role in the detoxification of alkenals produced from lipid peroxides, were induced in liver, small intestine, and kidney of Falcarindiol-treated mice. Additionally, we investigated the protective effects of Falcarindiol against hepatotoxicity induced by carbon tetrachloride (CCl(4)) and the mechanism of its hepatoprotective effect. Pretreatment with Falcarindiol prior to the administration of CCl(4) significantly suppressed both an increase in serum alanine transaminase/aspartate transaminase (ALT/AST) activity and an increase in hepatic thiobarbituric acid reactive substance levels without affecting CCl(4)-mediated degradation of CYP2E1. Formation of hexanoyl-lysine and 4-hydroxy-2(E)-nonenal-histidine adducts, lipid peroxidation biomarkers, in homogenates from the liver of CCl(4)-treated mice was decreased in the group of mice pretreated with Falcarindiol.
CONCLUSIONS:
These results suggest that the protective effects of Falcarindiol against CCl(4) toxicity might, in part, be explained by anti-lipid peroxidation activity associated with the induction of the GSTs including GSTA4. | | BMC Res Notes . 2018 Jun 27;11(1):411. | | Effect of the dietary polyacetylenes falcarinol and falcarindiol on the gut microbiota composition in a rat model of colorectal cancer[Pubmed: 29945666] | | Abstract
Objectives: (3R)-Falcarinol (FaOH) and (3R,8S)-Falcarindiol (FaDOH) have previously been shown to reduce the number of neoplastic lesions and the growth rate of polyps in the colon of azoxymethane (AOM) treated rats. Based on previous investigations, it appears that different mechanisms of actions are involved in the antineoplastic effect of FaOH and FaDOH. One mechanism of action may be related to the antibacterial effect of FaOH and FaDOH and thus their effect on the gut microbiota. This study aimed to determine the effect of FaOH and FaDOH on gut microbiota composition of AOM treated rats.
Results: Azoxymethane treated rats were fed either a standard rat diet or a rat diet supplemented with FaOH and FaDOH. The gut microbiota of AOM-induced rats was determined by 16S rRNA gene-amplicon sequencing. Analysis of fecal cecum samples demonstrated a significant gut microbiota change in rats receiving standard rat diet supplemented with FaOH and FaDOH compared with the control group that only received the rat diet. Comparison of the gut microbiota of rats who developed large neoplasms in the colon with rats without large neoplasms showed that the gut microbiota was significantly different in rats who developed large colon neoplasms compared to rats with no macroscopic colon neoplasms.
Keywords: Carrots; Colorectal cancer; Falcarindiol; Falcarinol; Microbiota; Polyacetylenes; Rat model. |
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