Humantenmine | CAS:82354-38-9 | Manufacturer ChemFaces

Humantenmine

ChemFaces products have been cited in many studies from excellent and top scientific journals

Order & Inquiry & Tech Support

Tel: (0086)-27-84237683
Tech: service@chemfaces.com
Order: manager@chemfaces.com
Address: 176, CheCheng Eest Rd., WETDZ, Wuhan, Hubei 430056, PRC

How to Order

Orders via your E-mail:

1. Product number / Name / CAS No.
2. Delivery address
3. Ordering/billing address
4. Contact information
Order: manager@chemfaces.com

Delivery time

Delivery & Payment method

1. Usually delivery time: Next day delivery by 9:00 a.m. Order now

2. We accept: Wire transfer & Credit card & Paypal

Citing Use of our Products

More Impact Factor than five Cite...

* Packaging according to customer requirements(5mg, 10mg, 20mg and more). We shipped via FedEx, DHL, UPS, EMS and others courier.

According to end customer requirements, ChemFaces provide solvent format. This solvent format of product intended use: Signaling Inhibitors, Biological activities or Pharmacological activities.

Size /Price /Stock	10 mM * 1 mL in DMSO / $109.4 / In-stock
Other Packaging	*Packaging according to customer requirements(100uL/well, 200uL/well and more), and Container use Storage Tube With Screw Cap

Featured Products

More...

p-Hydroxy-cinnamic acid

Catalog No: CFN98179
CAS No: 7400-08-0
Price: $70/20mg

Ganoderenic acid D

Catalog No: CFN90300
CAS No: 100665-43-8
Price: $368/20mg

Calycosin-7-O-beta-D-glucoside

Catalog No: CFN99141
CAS No: 20633-67-4
Price: $80/20mg

Cannabidivarin

Catalog No: CFN89253
CAS No: 24274-48-4
Price: $ / mg

Baicalein

Catalog No: CFN98783
CAS No: 491-67-8
Price: $40/20mg

Neoprzewaquinone A

Catalog No: CFN97087
CAS No: 630057-39-5
Price: $388/5mg

Mulberroside F

Catalog No: CFN90794
CAS No: 193483-95-3
Price: $288/10mg

Flavone

Catalog No: CFN70130
CAS No: 525-82-6
Price: $30/20mg

Ginkgolide B

Catalog No: CFN99640
CAS No: 15291-77-7
Price: $40/20mg

Resibufogenin

Catalog No: CFN98543
CAS No: 465-39-4
Price: $118/20mg

Description:

Humantenmine is a toxic compound isolated from Gelsemium elegans Benth. Humantenmine and koumine may inhibit several CYP450 enzyme activities.

Targets:

P450 (e.g. CYP17)

In vitro:

J Ethnopharmacol. 2015 May 26;166:66-73.

Inhibitory effects of cytochrome P450 enzymes CYP1A2, CYP2A6, CYP2E1 and CYP3A4 by extracts and alkaloids of Gelsemium elegans roots.[Pubmed: 25764964]

METHODS AND RESULTS:
Four extracts (petroleum ether, dichloromethane, EtOAc and aqueous) of GE and two commercially available alkaloids (koumine and Humantenmine) were screened for their CYP isoforms inhibitory activity. In the case of alkaloids, koumine exhibited significant inhibitory effects on CYP2E1 while Humantenmine showed more potent inhibition on CYP2E1 and CYP2A6 (IC50 of 47.44, 18.34 and 45.87µg/ml, Ki of 31.20, 35.06 and 52.06µg/ml, respectively). Because of their relatively high Ki values, the active constituents in GE dichloromethane extract were analyzed. The UPLC-DAD-ESI-MS/MS data showed that GE dichloromethane extract contains 6 kinds of indole alkaloids (koumine, Humantenmine, humantenine, humantenirine, N-methoxytaberpsychine, and sempervirine). As for CYP1A2 and CYP3A4, the negligible inhibitions were observed.
CONCLUSIONS:
G. elegans extracts inhibited several CYP450 enzyme activities with varying potency. Strong inhibition was observed in CYP2E1 and CYP2A6 isoforms by GE dichloromethane extract, koumine and Humantenmine, inferring the involvement of alkaloids chemical constituents from GE dichloromethane extract in the effect.

Humantenmine Description

Source:	The roots of Gelsemium elegans
Solvent:	Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.
Storage:	Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C). Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour. Need more advice on solubility, usage and handling? Please email to: service@chemfaces.com
After receiving:	The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.

Structure Identification:

J Sep Sci. 2014 Feb;37(3):308-13.

Ultrasound/microwave-assisted extraction and comparative analysis of bioactive/toxic indole alkaloids in different medicinal parts of Gelsemium elegans Benth by ultra-high performance liquid chromatography with MS/MS.[Pubmed: 24375943]

Indole alkaloids are the main bioactive/toxic components in Gelsemium elegans Benth.
METHODS AND RESULTS:
To determine the distribution and contents of indole alkaloids in its different medicinal parts, a novel and rapid method using ultra-high performance LC (UPLC) with MS/MS has been established and validated with an optimized ultrasound/microwave-assisted extraction method. Four constituents, namely, humantenidine, Humantenmine, gelsemine, and koumine, were simultaneously determined in 6 min. Chromatographic separation was achieved on an ultra-high performance LC BEH C18 column with a gradient mobile phase consisting of methanol and water (containing 0.1% formic acid both in methanol and water) at a flow rate of 0.3 mL/min. The detection was performed on a triple quadrupole electrospray MS/MS by positive ion multiple-reaction monitoring mode. All the analytes showed good linearity (r ≥ 0.9934) within a concentration range from 0.1-25 μg/mL with a LOQ of 25-50 ng/mL. The overall intra- and intervariations of four components were <4.7% with an accuracy of 97.3-101.3%.
CONCLUSIONS:
The analysis results showed that there were remarkable differences in the distribution and contents of four chemical markers in the roots, stems, and leaves of G. elegans Benth. The findings can provide necessary and meaningful information for the rational utilization of its resources.

Product Name	Humantenmine
Price:	$238 / 10mg
CAS No.:	82354-38-9
Catalog No.:	CFN97304
Molecular Formula:	C₁₉H₂₂N₂O₃
Molecular Weight:	326.4 g/mol
Purity:	>=98%
Type of Compound:	Alkaloids
Physical Desc.:	Powder
Source:	The roots of Gelsemium elegans
Solvent:	Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.
Download:	COA MSDS SDF
Similar structural:	Comparison (Web) (SDF)
Guestbook:

Size /Price /Stock	10 mM * 100 uL in DMSO / Inquiry / In-stock 10 mM * 1 mL in DMSO / Inquiry / In-stock
Related Libraries	Inhibitors Compound Library Toxicity Compound Library Alkaloids Compound Library P450 (e.g. CYP17) Inhibitor Library

	1 mg	5 mg	10 mg	20 mg	25 mg
1 mM	3.0637 mL	15.3186 mL	30.6373 mL	61.2745 mL	76.5931 mL
5 mM	0.6127 mL	3.0637 mL	6.1275 mL	12.2549 mL	15.3186 mL
10 mM	0.3064 mL	1.5319 mL	3.0637 mL	6.1275 mL	7.6593 mL
50 mM	0.0613 mL	0.3064 mL	0.6127 mL	1.2255 mL	1.5319 mL
100 mM	0.0306 mL	0.1532 mL	0.3064 mL	0.6127 mL	0.7659 mL

CFN97305	Humantenine	82375-29-9	354.5	C₂₁H₂₆N₂O₃
CFN97998	11-Hydroxyhumantenine	122590-04-9	370.5	C₂₁H₂₆N₂O₄
CFN98622	Gelsevirine	38990-03-3	352.4	C₂₁H₂₄N₂O₃
CFN99001	Gelsemine	509-15-9	322.4	C₂₀H₂₂N₂O₂
CFN99425	Koumine	1358-76-5	306.4	C₂₀H₂₂N₂O
CFN97937	Koumine N-oxide	113900-75-7	322.4	C₂₀H₂₂N₂O₂
CFN97880	Humantenidine	114027-39-3	342.4	C₁₉H₂₂N₂O₄
CFN97887	11-Hydroxygelsenicine	1195760-68-9	342.4	C₁₉H₂₂N₂O₄
CFN97944	11-Hydroxyrankinidine	122590-03-8	356.4	C₂₀H₂₄N₂O₄
CFN97983	Humantenirine	82375-30-2	370.5	C₂₁H₂₆N₂O₄