| Structure Identification: |
| Chinese Traditional & Herbal Drugs, 2015, 46(20):3091-5. | | Comparison on homoisoflavones extracted from Ophiopogon japonicus in Sichuan and Zhejiang and their anti-oxidative activity.[Reference: WebLink] | To compare the contents of three homoisoflavones and their anti-oxidative activity of Ophiopogon japonicus in Sichuan(OJS) and Zhejiang(OJZ).
METHODS AND RESULTS:
The determination was performed on ACQUITYTM UPLC BEH C18 column(100 mm × 2.1 mm, 1.7 μm), the Ultra Performance Liquid Chromatography-Photo-Diode Array(UPLC-PDA) technology was applied, and the mobile phase consisted of acetonitrile and 0.2% formic acid aqueous solution(55 : 45) with gradient elution program, flow rate was 0.20 m L/min with 2 μL of sample quantity at 296 nm, and the anti-DPPH radical efficiency of water extract from Ophiopogonis Radix were evaluated by UV-photometer. The average values of methylophiopogonone A, Methylophiopogonanone A, and methylophiopogonanone B were(3.06 ± 0.54),(40.10 ± 5.63), and(29.51 ± 5.06) μg/g in OJS, respectively; while those in OJZ were(9.22 ± 3.52),(106.63 ± 27.56), and(256.97 ± 61.79) μg/g, separately. The IC50 value was 16.59 mg/m L in OJS, while that in OJZ was 14.48 mg/m L. The IC50 value of positive control VC was 7.06 μg/m L.
CONCLUSIONS:
Compared with OJS, the contents of homoisoflavones in OJZ are higher, and the anti-radical efficiency of water extract from OJZ is stronger. It provides the basis for the quality evaluation and geo-authentic research of Ophiopogonis Radix. |
|
Cell. 2018 Jan 11;172(1-2):249-261.e12. doi: 10.1016/j.cell.2017.12.019.IF=36.216(2019)
Cell Metab. 2020 Mar 3;31(3):534-548.e5. doi: 10.1016/j.cmet.2020.01.002.IF=22.415(2019)
Mol Cell. 2017 Nov 16;68(4):673-685.e6. doi: 10.1016/j.molcel.2017.10.022.IF=14.548(2019)
ACS Nano. 2018 Apr 24;12(4): 3385-3396. doi: 10.1021/acsnano.7b08969.IF=13.903(2019)
Nature Plants. 2016 Dec 22;3: 16206. doi: 10.1038/nplants.2016.205.IF=13.297(2019)
Sci Adv. 2018 Oct 24;4(10): eaat6994. doi: 10.1126/sciadv.aat6994.IF=12.804(2019)