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N-Nornuciferine
N-Nornuciferine
ChemFaces products have been cited in many studies from excellent and top scientific journals
Product Name N-Nornuciferine
Price: $338 / 20mg
CAS No.: 4846-19-9
Catalog No.: CFN96149
Molecular Formula: C18H19NO2
Molecular Weight: 281.4 g/mol
Purity: >=98%
Type of Compound: Alkaloids
Physical Desc.: Powder
Source: The fruits of Piper nigrum
Solvent: Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.
Download: COA    MSDS    SDF
Similar structural: Comparison (Web)  (SDF)
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Size /Price /Stock 10 mM * 1 mL in DMSO / $64.5 / In-stock
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Related Screening Libraries
Size /Price /Stock 10 mM * 100 uL in DMSO / Inquiry / In-stock
10 mM * 1 mL in DMSO / Inquiry / In-stock
Related Libraries
Biological Activity
Description: N-Nornuciferine can competitively inhibit CYP2D6 activity with Ki values of 2.34 uM.
Targets: P450 (e.g. CYP17)
In vitro:
J Ethnopharmacol. 2014 Apr 11;153(1):190-6.
Identification and characterization of potent CYP2D6 inhibitors in lotus leaves.[Pubmed: 24561383]

METHODS AND RESULTS:
Five probe substrates were incubated with human liver microsomes in the presence or absence of the LAE, the alkaloid fraction (AF), the flavonoid fraction (FF), or the individual aporphine alkaloids, namely, nuciferine (NF), N-Nornuciferine (N-NF), and 2-hydroxy-1-methoxyaporphine (HMA). After the incubation, the relative metabolites of the substrates were analyzed using LC-MS/MS. The results showed that the LAE strongly inhibited CYP2D6 with an IC50 value of 12.05µg/mL and weakly inhibited other isoenzymes. In addition, FF was found to weakly inhibit CYP2D6, whereas AF exerted a markedly higher inhibitory effect on CYP2D6 activity with an IC50 value of 0.96µg/mL. The three aporphine alkaloids isolated from the AF (NF, N-Nornuciferine, and HMA) significantly inhibited CYP2D6 with IC50 values of 3.78, 3.76, and 3.15µM, respectively.
CONCLUSIONS:
Their Lineweaver-Burk plots and Dixon plots showed that NF, N-Nornuciferine, and HMA competitively inhibited CYP2D6 activity with Ki values of 1.88, 2.34, and 1.56µM, respectively.
N-Nornuciferine Description
Source: The fruits of Piper nigrum
Solvent: Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.
Storage: Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).

Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

Need more advice on solubility, usage and handling? Please email to: service@chemfaces.com

After receiving: The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
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Recently, ChemFaces products have been cited in many studies from excellent and top scientific journals

Cell. 2018 Jan 11;172(1-2):249-261.e12.
doi: 10.1016/j.cell.2017.12.019.
IF=36.216(2019)

PMID: 29328914

Cell Metab. 2020 Mar 3;31(3):534-548.e5.
doi: 10.1016/j.cmet.2020.01.002.
IF=22.415(2019)

PMID: 32004475

Mol Cell. 2017 Nov 16;68(4):673-685.e6.
doi: 10.1016/j.molcel.2017.10.022.
IF=14.548(2019)

PMID: 29149595

ACS Nano. 2018 Apr 24;12(4): 3385-3396.
doi: 10.1021/acsnano.7b08969.
IF=13.903(2019)

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Nature Plants. 2016 Dec 22;3: 16206.
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PMID: 28005066

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Calculate Dilution Ratios(Only for Reference)
1 mg 5 mg 10 mg 20 mg 25 mg
1 mM 3.5537 mL 17.7683 mL 35.5366 mL 71.0732 mL 88.8415 mL
5 mM 0.7107 mL 3.5537 mL 7.1073 mL 14.2146 mL 17.7683 mL
10 mM 0.3554 mL 1.7768 mL 3.5537 mL 7.1073 mL 8.8842 mL
50 mM 0.0711 mL 0.3554 mL 0.7107 mL 1.4215 mL 1.7768 mL
100 mM 0.0355 mL 0.1777 mL 0.3554 mL 0.7107 mL 0.8884 mL
* Note: If you are in the process of experiment, it's need to make the dilution ratios of the samples. The dilution data of the sheet for your reference. Normally, it's can get a better solubility within lower of Concentrations.
Protocol
Structure Identification:
Talanta. 2010 Jan 15;80(3):1292-7.
Application of ionic liquids based microwave-assisted extraction of three alkaloids N-nornuciferine, O-nornuciferine, and nuciferine from lotus leaf.[Pubmed: 20006090]
The application of ionic liquids based microwave-assisted extraction (ILMAE) was successfully developed for extracting three alkaloids N-Nornuciferine, O-nornuciferine, and nuciferine from lotus leaf.
METHODS AND RESULTS:
Seven kinds of 1-alkyl-3-methylimidazolium with different cations and anions were investigated in this work and 1.0M 1-hexyl-3-methylimidazolium bromide ([C(6)MIM]Br) solution was selected as solvent. In addition, the microwave parameters including irradiation power, extraction time and solid-liquid ratio were optimized. Compared with the regular MAE and conventional heat-reflux extraction (HRE), the proposed approach exhibited higher efficiency (0.9-43.7% enhanced) and shorter extraction time (from 2h to 2min), which indicated ILMAE was an efficient, rapid and simple sample preparation technique. Moreover, the proposed method was validated by the linearity, reproducibility, and recovery experiments. Good linearity was observed with the regression coefficients (r(2)) between 0.9998 and 0.9999.
CONCLUSIONS:
The recoveries of all methods were in the range of 94.6% and 105.5% with RSD lower than 6.6%, which indicated that the proposed method was credible.
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