|Description:||1. Rhoifolin and cosmosiin from red wendun leaves may be beneficial for diabetic complications through their enhanced adiponectin secretion, tyrosine phosphorylation of insulin receptor-β and GLUT4 translocation. |
2. Rhoifolin has anti-inflammatory activity and increases the total antioxidant capacity in a reverse dose order, it can cause a time and reverse dose dependent reduction of carrageenin-induced rat paw oedema, significantly abrogate prostaglandin E2 level, significantly diminish the TNF-a release in the inflammatory exudates.
3. Rhoifolin could be used as an ideal anticancer agent, it discriminates between cancerous and non cancerous cell as it kills only the former one, so the side effects which may appear during chemotherapy could be overcome.
4. Rhoifolin has a radioprotective effect against radiation-induced decrease of blood platelets and cardiac biochemical lesions in whole body irradiated mice.
5. Rhoifolin has protective effect against hepatic injury induced by triptolide.
6. Rhoifolin shows monolithic proliferation inhibitory activities against Hela, A549 and MCF-7 cell lines.
|Targets:||GLUT | TNF-α | PGE|
|Source:||The leaves of Turpinia arguya Seem|
|Solvent:||DMSO, Pyridine, Methanol, Ethanol, etc.|
|Storage:||Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please email to: firstname.lastname@example.org
|After receiving:||The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.|
|1 mg||5 mg||10 mg||20 mg||25 mg|
|1 mM||1.7286 mL||8.643 mL||17.2861 mL||34.5722 mL||43.2152 mL|
|5 mM||0.3457 mL||1.7286 mL||3.4572 mL||6.9144 mL||8.643 mL|
|10 mM||0.1729 mL||0.8643 mL||1.7286 mL||3.4572 mL||4.3215 mL|
|50 mM||0.0346 mL||0.1729 mL||0.3457 mL||0.6914 mL||0.8643 mL|
|100 mM||0.0173 mL||0.0864 mL||0.1729 mL||0.3457 mL||0.4322 mL|
Evid Based Complement Alternat Med. 2011;2011:624375.
|Insulin-Mimetic Action of Rhoifolin and Cosmosiin Isolated from Citrus grandis (L.) Osbeck Leaves: Enhanced Adiponectin Secretion and Insulin Receptor Phosphorylation in 3T3-L1 Cells.[Pubmed: 20008903]|
|Two flavone glycosides, Rhoifolin and cosmosiin were isolated for the first time from red wendun leaves and, identified these leaves are rich source for Rhoifolin (1.1%, w/w). In differentiated 3T3-L1 adipocytes, Rhoifolin and cosmosiin showed dose-dependent response in concentration range of o.oo1-5 μM and 1-20 μM, respectively, in biological studies beneficial to diabetes. Particularly, Rhoifolin and cosmosiin at 0.5 and 20 μM, respectively showed nearly similar response to that 10 nM of insulin, on adiponectin secretion level. Furthermore, 5 μM of Rhoifolin and 20 μM of cosmosiin showed equal potential with 10 nM of insulin to increase the phosphorylation of insulin receptor-β, in addition to their positive effect on GLUT4 translocation. These findings indicate that Rhoifolin and cosmosiin from red wendun leaves may be beneficial for diabetic complications through their enhanced adiponectin secretion, tyrosine phosphorylation of insulin receptor-β and GLUT4 translocation.|
J. Appl. Pharmaceut. Sci., 2012, 2(8):74-9.
|Anti-inflammatory effect of Apigenin-7-neohesperidoside (Rhoifolin) in carrageenin-induced rat edema model[Reference: WebLink]|
|Flavonoids are normal constituents of the human diet and are known for a variety of biological activities. They have been reported to bring benefits in lowering inflammation and oxidative stress. The present investigation was performed first, to evaluate the anti-inflammatory activity of Rhoifolin and second, to search for the possible contributing mechanisms for this hypothesized effect. Rhoifolin caused a time and reverse dose dependent reduction of carrageenin-induced rat paw oedema. Following 4 hr of treatment, Rhoifolin at doses 2.5, 25 & 250 mg/kg caused a significant inhibition of rat paw edema volume by 14, 25 & 45 % respectively in comparison to the control group (74%). In addition to significantly abrogating prostaglandin E2 level, increasing doses of Rhoifolin significantly diminished the TNF-a release in the inflammatory exudates. In the same animal model, Rhoifolin increased the total antioxidant capacity in a reverse dose order, with the highest capacity obtained with the lowest dose tested. This study demonstrates for the first time the effectiveness.|
Zhongguo Zhong Yao Za Zhi. 2014 Aug;39(16):3094-101.
|[Chemical constituents from Callicarpa nudiflora and their cytotoxic activities].[Pubmed: 25509294]|
|Luteoloside(1), lutedin-4'-O-β-D-glucoside(2), 6-hydroxyluteolin-7-O-β-glucoside(3), lutedin-7-O-neohesperidoside(4), Rhoifolin (5), luteolin-7, 4'-di-O-glucoside (6), forsythoside B (7), acteoside (8), alyssonoside (9), catalpol(10), nudifloside(11), and leonuride(12). Compounds 3-6, 10 and 12 were isolated from this genus for the first time, and compound 9 was isolated from this plant for the first time. The cytotoxicity assay demonstrated that flavonoids 1-6, in various concentrations, showed monolithic proliferation inhibitory activities against Hela, A549 and MCF-7 cell lines. Compounds 3, 5 and iridoid glycoside 11 possessed higher cytotoxicacivities. In short, flavonoids are the main components of cytotoxic extract from C. nudiflora, while phenylethanoid glycosides are the predominant ingredient but inactive to cancer cell lines. In addition, the minor iridoid glycoside expressed weak cytotoxic activity.|
Brit. J. Pharmaceut. Res., 2013, 3(1):46-53.
|Rhoifolin; A Potent Antiproliferative Effect on Cancer Cell Lines[Reference: WebLink]|
|Rhoifolin, Apigenin 7-O-β neohesperidoside was isolated in a copious amount from the leaves of Chorisia crispiflora (Bombaceae). Its identity was unambiguously confirmed via different spectroscopic methods (UV, 1 HNMR, 13 CNMR and HMBC) and Viability assay test was used to evaluate its cytotoxic activity. Results: It exhibited potent anticancer activities, nearly similar to that of vinblastine, when evaluated against human epidermoid larynex (Hep 2) and human cervical (HeLa) carcinoma cell lines. Promising activities were also obtained against hepatocellular (Hep G2), colon (HCT-116) and fetal human lung fibroblast (MRC-5) carcinoma cell lines. A unique effect of Rhoifolin was in having no cytotoxic activity against healthy normal cells (Vero cells) which indicates a high selectivity of the compound selected. Conclusion: The findings of this study showed that Rhoifolin could be used as an ideal anticancer agent. It discriminates between cancerous and non cancerous cell as it kills only the former one. So the side effects which may appear during chemotherapy could be overcome.|
Journal of Nuclear Science and Applications, 2014,47(1):198-204.
|Protective Effect of Rhoifolin on Gamma Irradiation Induced Cardiac Dysfunctions in Albino Mice.[Reference: WebLink]|
|Rhoifolin (apigenin 7 neohesperidoside) is a flavone glycoside, a natural product belonging to the flavonoid. This study aimed to estimate the optimum dose level of Rhoifolin against the lethality of 10Gy irradiation dose in mice. Also, to evaluate the modulator effect of Rhoifolin against 10 Gy irradiation induced certain haematological and cardiac biochemical abnormalities. Six groups of mice (n =15) were used. The 1 st group subjected to a single dose (10 Gy) irradiation and used as control. The other five groups administered different doses of Rhoifolin (12, 24, 30, 36, and 40 mg/kg) po for 7 consecutive days pre irradiation (10 gray). The optimum dose of Rhoifolin against radiation induced mortality and body weight loss was evaluated and found to be 36 mg/kg body weight. Conclusion: The present study suggests a radioprotective effect of Rhoifolin against radiation-induced decrease of blood platelets and cardiac biochemical lesions in whole body irradiated mice.|
Pharmacology & Clinics of Chinese Materia Medica, 2012(6):18-20.
|Study on the protective effect of rhoifolin against hepatic injury induced by triptolide[Reference: WebLink]|
|The cell survival rates of human L02 liver cells injured by triptolide and then treated with Rhoifolin at the concentration of 75,150,300μg/ml were significantly increased.The contents of ALT,AST in serum were significantly decreased,the content of MDA in the liver was also significantly lowered,and the activities of SOD,MDA,GSH-Px and GST in liver were significantly improved after preventive treatment with Rhoifolin at the dose of 140,70,35mg/kg.Rhoifolin can ameliorated the hepatic pathological changes.Conclusion: The protective effect of Rhoifolin against hepatic injury induced by triptolide was significant in vitro and in vivo.|