| Kinase Assay: |
| Pharmacol Res. 2010 Jun;61(6):519-24. | | Discovery of cyclooxygenase inhibitors from medicinal plants used to treat inflammation.[Pubmed: 20188172 ] |
METHODS AND RESULTS:
Eleven authenticated botanicals used in the traditional Chinese medicine Huo-Luo-Xiao-Ling Dan were screened for ligands to cyclooxygenase (COX) using pulsed ultrafiltration liquid chromatography-mass spectrometry, and a mass spectrometry-based enzyme assay was used to determine the concentration of each of 17 ligands that inhibited COX-1 or COX-2 by 50% (IC(50)).
CONCLUSIONS:
Acetyl-11-keto-beta-boswellic acid, beta-boswellic acid, acetyl-alpha-boswellic acid, acetyl-beta-boswellic acid, and betulinic acid were COX-1 selective inhibitors with IC(50) values of approximately 10 microM. Senkyunolide O and cryptotanshinone were COX-2 selective inhibitors with IC(50) values of 5 microM and 22 microM, respectively. Roburic acid and phenethyl-trans-ferulate inhibited COX-1 and COX-2 equally. COX inhibition and the IC(50) values of most of these natural product ligands have not been reported previously. |
|
| Structure Identification: |
| Zhongguo Zhong Yao Za Zhi. 2008 Oct;33(19):2196-201. | | Study on biligustilides from Angelica sinensis.[Pubmed: 19166005] | To study the chemical constituents of Angelica sinensis.
METHODS AND RESULTS:
The constituents were separated by chromatographic methods, and their structures were identified on the basis of spectroscopic analysis. Eight compounds were isolated and identified as levistolide A (1), Senkyunolide O (2), (3Z, 3Z')-6.8', 7.3'-diligustilide (3), tokinolide B (4), isotokinolide B (5), (3'Z)-(3R, 8S, 3a'R, 6'S)-3, 3a': 8, 6'-biligustilide (6), E, E'-3. 3', 8. 8'-diligustilide (7) and E, E'-3. 3', 8. 8'-isodiligustilide (8), which are all diligustilides.
CONCLUSIONS:
Compound 7 was obtained from the plant for the first time; compounds 6 and 8 are new compounds. |
|
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