| Kinase Assay: |
| Cell Mol Immunol. 2014 Oct 13. | | Modulation of IL-37 expression by triptolide and triptonide in THP-1 cells.[Pubmed: 25308753] | IL-37 is an anti-inflammatory cytokine that was only recently identified, and it is highly expressed in tissues from patients with inflammatory and autoimmune diseases. Inflammatory cytokines and inflammatory stimuli can induce the upregulation of IL-37. However, it has not been reported whether anti-inflammatory medications induce the expression of IL-37.
METHODS AND RESULTS:
In this work, we uncovered, for the first time, that two main bioactive components, triptolide and Triptonide, from the herb Tripterygium wilfordii Hook f. (TwHF), which possess anti-inflammatory activity, upregulate the expression of IL-37, and this expression was suppressed by ERK1/2 and p38 MAPK inhibitors.
CONCLUSIONS:
Overall, our research demonstrated, for the first time, that anti-inflammatory active components (triptolide and Triptonide) upregulated the expression of IL-37 most likely via activation of the ERK1/2 and p38 MAPK pathways.C | | Sci Rep. 2016 Sep 6;6:32779. | | Triptonide Effectively Inhibits Wnt/β-Catenin Signaling via C-terminal Transactivation Domain of β-catenin.[Pubmed: 27596363] | | Abnormal activation of canonical Wnt/β-catenin signaling is implicated in many diseases including cancer. As a result, therapeutic agents that disrupt this signaling pathway have been highly sought after. Triptonide is a key bioactive small molecule identified in a traditional Chinese medicine named Tripterygium wilfordii Hook F., and it has a broad spectrum of biological functions. Here we show that Triptonide can effectively inhibit canonical Wnt/β-catenin signaling by targeting the downstream C-terminal transcription domain of β-catenin or a nuclear component associated with β-catenin. In addition, Triptonide treatment robustly rescued the zebrafish "eyeless" phenotype induced by GSK-3β antagonist 6-bromoindirubin-30-oxime (BIO) for Wnt signaling activation during embryonic gastrulation. Finally, Triptonide effectively induced apoptosis of Wnt-dependent cancer cells, supporting the therapeutic potential of Triptonide. |
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| Animal Research: |
| Chinese Journal of Hospital Pharmacy, 2006, 26(6):695-7. | | Effect of triptonide on inflammation and immunity function.[Reference: WebLink] |
METHODS AND RESULTS:
To study the effect of Triptonide on inflammation and immunity function.METHODS The effect of Triptonide on acute auricle inflammation,capillary permeability,the index of immune organ,the clearance of charcoal particles,the level of spleen cell antibody,the level of serum hemolysin and T-lymphocytes percentage were observed in mice by ig.RESULTS Triptonide could apparently inhibit auricle inflammation and capillary permeability,decrease the index of thymus and the clearance rate of charcoal particles and suppress the formation of spleen cell antibody,reduce the content of serum hemolysin and T-lymphocytes percentage in mice.
CONCLUSIONS:
Triptonide has good anti-inflammatory and immunosuppressive action. |
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