|Source:||The herbs of Guazuma ulmifolia|
|Biological Activity or Inhibitors:||1. 7-Amino-4-methylcoumarin is more suitable as a substrate for enteropeptidase than GD(4)K-NA.
|Solvent:||Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.|
|Storage:||Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please email to: firstname.lastname@example.org
|After receiving:||The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.|
|1 mg||5 mg||10 mg||20 mg||25 mg|
|1 mM||4.2878 mL||21.439 mL||42.878 mL||85.7559 mL||107.1949 mL|
|5 mM||0.8576 mL||4.2878 mL||8.5756 mL||17.1512 mL||21.439 mL|
|10 mM||0.4288 mL||2.1439 mL||4.2878 mL||8.5756 mL||10.7195 mL|
|50 mM||0.0858 mL||0.4288 mL||0.8576 mL||1.7151 mL||2.1439 mL|
|100 mM||0.0429 mL||0.2144 mL||0.4288 mL||0.8576 mL||1.0719 mL|
BMB Rep. 2011 Jul;44(7):458-61.
|A fluorogenic method for measuring enteropeptidase activity: spectral shift in the emission of GD4K-conjugated 7-amino-4-methylcoumarin.[Pubmed: 21777516]|
|We used shift in the emission spectrum of GD(4)K-conjugated 7-Amino-4-methylcoumarin (GD(4)K-AMC) as a fluorogenic method to measure enteropeptidase activity. The kinetic analysis revealed that enteropeptidase has a K(M) of 0.025 mM and a k(cat) of 65 sec(-1) for 7-Amino-4-methylcoumarin, whereas it has a K(M) of 0.5 to 0.6 mM and a k(cat) of 25 sec(-1) for GD(4)K-NA. The optimum pH of 7-Amino-4-methylcoumarin hydrolysis was pH 8.0. Our data indicate that 7-Amino-4-methylcoumarin is more suitable as a substrate for enteropeptidase than GD(4)K-NA.|