• ChemFaces is a professional high-purity natural products manufacturer.
  • Product Intended Use
  • 1. Reference standards
  • 2. Pharmacological research
  • 3. Inhibitors
  • Home
  • Natural Products
  • Bioactive
  • Screening Libraries
  • Hot Products
  • Plant Catalog
  • Customer Support
  • Product Use Citation
  • About Us
  • Contact Us
  • Natural Products
    CAS No. 70553-76-3 Price $238 / 20mg
    Catalog No.CFN90534Purity>=98%
    Molecular Weight610.73Type of CompoundAlkaloids
    FormulaC37H42N2O6Physical DescriptionPowder
    Download     COA    MSDSSimilar structuralComparison (Web)
    Citing Use of our Products
    How to Order
    Orders via your E-mail:

    1. Product number / Name / CAS No.
    2. Delivery address
    3. Ordering/billing address
    4. Contact information
    Sent to Email: info@chemfaces.com
    Contact Us
    Order & Inquiry & Tech Support

    Tel: (0086)-27-84237683
    Fax: (0086)-27-84254680
    E-mail: manager@chemfaces.com
    Address: No. 83, CheCheng Rd., WETDZ, Wuhan, Hubei 430056, PRC
    Delivery time
    Delivery & Payment method

    1. Usually delivery time: Next day delivery by 9:00 a.m. Order now

    2. We accept: Wire transfer & Credit card & Paypal & Western Union
    * Packaging according to customer requirements(5mg, 10mg, 20mg and more). We shipped via FedEx, DHL, UPS, EMS and others courier.
    According to end customer requirements, ChemFaces provide solvent format. This solvent format of product intended use: Signaling Inhibitors, Biological activities or Pharmacological activities.
    Size /Price /Stock 10 mM * 1 mL in DMSO / $245.5 / In-stock
    Other Packaging *Packaging according to customer requirements(100uL/well, 200uL/well and more), and Container use Storage Tube With Screw Cap
    Our products had been exported to the following research institutions and universities, And still growing.
  • University Medical Center Mainz (Germany)
  • University of Wisconsin-Madison (USA)
  • Mendel University in Brno (Czech Republic)
  • John Innes Centre (United Kingdom)
  • Harvard University (USA)
  • Helmholtz Zentrum München (Germany)
  • Research Unit Molecular Epigene... (Germany)
  • Istanbul University (Turkey)
  • VIT University (India)
  • Wroclaw Medical University (Poland)
  • University of Brasilia (Brazil)
  • More...
  • Package
    Featured Products

    Catalog No: CFN99710
    CAS No: 51059-44-0
    Price: $100/20mg
    3-Epioleanolic acid

    Catalog No: CFN92068
    CAS No: 25499-90-5
    Price: $388/10 mg

    Catalog No: CFN92016
    CAS No: 53846-50-7
    Price: $298/10mg
    Sibiricose A5

    Catalog No: CFN90645
    CAS No: 107912-97-0
    Price: $138/20mg

    Catalog No: CFN92154
    CAS No: 69618-96-8
    Price: $463/5mg

    Catalog No: CFN99106
    CAS No: 40957-83-3
    Price: $128/20mg
    Deapi-platycoside E

    Catalog No: CFN92355
    CAS No: 849758-42-5
    Price: $498/10mg
    Related Screening Libraries
    Size /Price /Stock 10 mM * 100 uL in DMSO / Inquiry / In-stock
    10 mM * 1 mL in DMSO / Inquiry / In-stock
    Related Libraries
  • Antiarrhythmic Compound Library
  • Alkaloids Compound Library
  • Sodium Channel Inhibitor Library
  • Potassium Channel Inhibitor Library
  • Calcium Channel Inhibitor Library
  • Biological Activity
    Description: Daurisoline is a hERG inhibitor and also an autophagy blocker.Daurisoline has antiarrhythmic effects, it( at concentrations below 30 uM) exerts a blocking effect on hERG, but does not affect the expression and function of the hERG channel, it also can inhibit early afterdepolarizations (EADs) which may be associated with its blockade effects on I(Ca-L). Daurisoline and its isomers show cytoprotective effects on ischemic injury in cultured pheochromocytoma (PC12) cells, which are mediated by blocking Ca2+ influx into cells.
    Targets: Calcium Channel | Sodium Channel | Potassium Channel | hERG
    In vitro:
    Am J Chin Med. 2010;38(1):37-49.
    Daurisoline suppressed early afterdepolarizations and inhibited L-type calcium current.[Pubmed: 20128043]
    Our previous studies have shown that Daurisoline (DS) exerted antiarrhythmic effects on various experimental arrhythmias. In this study, the effects of Daurisoline on early afterdepolarizations (EADs) and its possible mechanisms have been investigated. Cardiac hypertrophy was induced in rabbits by coarctating the abdominal aorta.
    The effects of Daurisoline on action potential duration (APD) and the incidences of EADs were studied in hypertrophied papillary muscles of rabbits in the conditions of low external K(+) concentration ([K(+)]o) and dofetilide (dof) by using standard microelectrode technique. The whole-cell patch clamp was used to record the L-type calcium current (I(Ca-L)) in isolated left ventricular cells of rabbits. The results showed that in hypertrophied papillary muscles of rabbits with low [K(+)]o ([K(+)]o = 2.7 mM), 1 microM dof prolonged APD(50) and APD(90) markedly and the incidence of EADs was 66.7% (4/6, p < 0.01); when 15 microM Daurisoline was applied, the incidence of EADs was 0% (0/4, p < 0.01) and the prolonged APD was shortened (p < 0.01). In a single myocyte, Daurisoline could also inhibit EADs induced by dof, low [K(+)]o and low external Mg(2+) concentration ([Mg(2+)]o) ([Mg(2+)](o) = 0.5 mM). Daurisoline could decrease the triangulation. In a single myocyte, Daurisoline could make the I-V curve upward, shift the steady-state activation curves to the right and the steady-state inactivation curves to the left and prolong the tau value of recovery curve obviously.
    These results suggested that Daurisoline could inhibit EADs which may be associated with its blockade effects on I(Ca-L).
    Zhongguo Yao Li Xue Bao. 1996 May;17(3):248-51.
    Effects of daurisoline on intracellular Ca2+ activity in myocardium.[Pubmed: 9812749]
    To explain the effect of Daurisoline (DS) on delayed afterdepolarization (DAD).
    Ca(2+)-sensitive microelectrode technic was used to record intracellular Ca2+ activity (alpha Cai) and triggered activity (TA) arising from DAD in myocardium. Strophantin G 3 mumol.L-1 yielded an increase in resting myocardial alpha Cai by 0.19 +/- 0.11 mumol.L-1 and transient elevations of alpha Cai by 1.48 +/- 0.55 and 4.96 +/- 1.81 mumol.L-1, respectively during the development of DAD and TA. By pretreatment with DS or verapamil, strophantin G-caused elevations of the alpha Cai in resting and provoked myocardia were eliminated and TA disappeared. DS 50 mumol.L-1 reduced Na(+)-free medium-induced elevation of dog Purkinje fibrous alpha Cai and abolished caffeine-induced increase of dog myocardial alpha Cai.
    DS inhibited DAD and TA by preventing an increase of alpha Cai via transmembrane Ca2+ entry and Ca2+ release from the reticulum.
    Daurisoline Description
    Source: The roots of Menispermum dauricum
    Solvent: Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.
    Storage: Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Need more advice on solubility, usage and handling? Please email to: service@chemfaces.com

    After receiving: The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
    Recent ChemFaces New Products and Compounds

    Catalog No: CFN92172
    CAS No: 22688-79-5
    Price: $198/10mg
    8-Hydroxypinoresinol diglucoside

    Catalog No: CFN95083
    CAS No: 112747-99-6
    Price: $318/5mg

    Catalog No: CFN95091
    CAS No: 168035-01-6
    Price: $288/5mg
    Carasinol B

    Catalog No: CFN95042
    CAS No: 777857-86-0
    Price: $333/5mg

    Catalog No: CFN90792
    CAS No: 23013-84-5
    Price: $398/10mg

    Catalog No: CFN98006
    CAS No: 19879-30-2
    Price: $128/20mg
    Picrasidine I

    Catalog No: CFN99019
    CAS No: 100234-59-1
    Price: $368/5mg

    Catalog No: CFN90448
    CAS No: 14941-08-3
    Price: $218/20mg
    Recently, ChemFaces products have been cited in many studies from excellent and top scientific journals

    Cell. 2018 Jan 11;172(1-2):249-261.e12.
    doi: 10.1016/j.cell.2017.12.019.

    PMID: 29328914

    Cell Metab. 2020 Mar 3;31(3):534-548.e5.
    doi: 10.1016/j.cmet.2020.01.002.

    PMID: 32004475

    Mol Cell. 2017 Nov 16;68(4):673-685.e6.
    doi: 10.1016/j.molcel.2017.10.022.

    PMID: 29149595

    ACS Nano. 2018 Apr 24;12(4): 3385-3396.
    doi: 10.1021/acsnano.7b08969.

    PMID: 29553709

    Nature Plants. 2016 Dec 22;3: 16206.
    doi: 10.1038/nplants.2016.205.

    PMID: 28005066

    Sci Adv. 2018 Oct 24;4(10): eaat6994.
    doi: 10.1126/sciadv.aat6994.

    PMID: 30417089
    Calculate Dilution Ratios(Only for Reference)
    1 mg 5 mg 10 mg 20 mg 25 mg
    1 mM 1.6374 mL 8.1869 mL 16.3738 mL 32.7477 mL 40.9346 mL
    5 mM 0.3275 mL 1.6374 mL 3.2748 mL 6.5495 mL 8.1869 mL
    10 mM 0.1637 mL 0.8187 mL 1.6374 mL 3.2748 mL 4.0935 mL
    50 mM 0.0327 mL 0.1637 mL 0.3275 mL 0.655 mL 0.8187 mL
    100 mM 0.0164 mL 0.0819 mL 0.1637 mL 0.3275 mL 0.4093 mL
    * Note: If you are in the process of experiment, it's need to make the dilution ratios of the samples. The dilution data of the sheet for your reference. Normally, it's can get a better solubility within lower of Concentrations.
    Kinase Assay:
    J Nat Prod. 2012 Sep 28;75(9):1539-45.
    Effect of daurisoline on HERG channel electrophysiological function and protein expression.[Pubmed: 22974355]
    Daurisoline (1) is a bis-benzylisoquinoline alkaloid isolated from the rhizomes of Menispermum dauricum. The antiarrhythmic effect of 1 has been demonstrated in different experimental animals.
    In previous studies, Daurisoline (1) prolonged action potential duration (APD) in a normal use-dependent manner. However, the electrophysiological mechanisms for Daurisoline-induced prolongation of APD have not been documented. In the present study, the direct effect of Daurisoline was investigated on the hERG current and the expression of mRNA and protein in human embryonic kidney 293 (HEK293) cells stably expressing the hERG channel. It was shown that Daurisoline inhibits hERG current in a concentration- and voltage-dependent manner. In the presence of 10 μM Daurisoline, steady-state inactivation of V(1/2) was shifted negatively by 15.9 mV, and Daurisoline accelerated the onset of inactivation. Blockade of hERG channels was dependent on channel opening. The expression and function of hERG were unchanged by Daurisoline at 1 and 10 μM, while hERG expression and the hERG current were decreased significantly by Daurisoline at 30 μM.
    These results indicate that Daurisoline, at concentrations below 30 μM, exerts a blocking effect on hERG, but does not affect the expression and function of the hERG channel. This may explain the relatively lower risk of long QT syndrome after long-term usage.
    Cell Research:
    Yao Xue Xue Bao. 1998;33(3):165-70.
    Effects of daurisoline and its three optical isomers on ischemic injury in cultured pheochromocytoma (PC12) cells.[Pubmed: 11938959]
    In this study, the protective effects of (-)-R.R-Daurisoline and its three optical isomers on ischemic injury in cultured PC12 cells induced by treating cells with NaCN in glucose-free medium were investigated.
    Cell viability was measured using MTT assay. The results indicated that these compounds, especially (-)-S.R and (+)-R.S isomers were found substantially to attenuate ischemic injury in PC12 cells in a dose-dependent manner. The IC50 values of (-)-R.R, (-)-S.R, (+)-R.S and (+)-S.S isomers were shown to be 18.6 x 10(-6), 2.4 x 10(-6), 5.9 x 10(-6) and 90 x 10(-6) mol.L-1, respectively. Intracellular free Ca2+ concentration in PC12 cells was measured using AR-CM-MIC cation measurement system with Fura-2/AM as Ca2+ fluorescent indicator. (-)-R.R-Daurisoline and its three optical isomers: (-)-S.R, (+)-R.S and (-)-S.S were found to markedly inhibit the increase of cytosolic free Ca2+ concentration induced by NaCN (20 mmol.L-1) in a dose-dependent manner. Their IC50 were found to be 3.55 x 10(-6), 0.59 x 10(-6), 1.29 x 10(-6) and 24.3 x 10(-6) mol.L-1 respectively.
    It is suggested that the cytoprotective effects of Daurisoline and its isomers were mediated by blocking Ca2+ influx into cells.