|Source:||The root barks of Dictamnus dasycarpus Turcz.|
|Biological Activity or Inhibitors:||1. Dictamnine and gamma-fagarine have mutagenic activities, they have specific activities (His+/microgram) of about 50-70 revertant colonies in strain TA100, while in strain TA98 there were about 30-50 revertant colonies.
2. Dictamnine has good antifungal activity alone and in combination with fluconazole against Candida albicans.
3. Dictamnine at higher concentrations(≥100uM) has potential hepatotoxicity, the cell membrane damage and mitochondrial membrane damage may be involved in the dictamnine-induced hepatotoxity mechanism.
4. Dictamnine has photoinduced genotoxicity.
5. Dictamnine shows anti-inflammatory effect.
6. Dictamnine shows anticholinesterase activityt.
|Solvent:||Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.|
|Storage:||Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please email to: email@example.com
|After receiving:||The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.|
|1 mg||5 mg||10 mg||20 mg||25 mg|
|1 mM||5.0198 mL||25.0991 mL||50.1983 mL||100.3966 mL||125.4957 mL|
|5 mM||1.004 mL||5.0198 mL||10.0397 mL||20.0793 mL||25.0991 mL|
|10 mM||0.502 mL||2.5099 mL||5.0198 mL||10.0397 mL||12.5496 mL|
|50 mM||0.1004 mL||0.502 mL||1.004 mL||2.0079 mL||2.5099 mL|
|100 mM||0.0502 mL||0.251 mL||0.502 mL||1.004 mL||1.255 mL|
Rev. Bras. Farmacogn., 2012, 22(2):374-80.
|Anticholinesterase activity evaluation of alkaloids and coumarin from stems of Conchocarpus fontanesianus[Reference: WebLink]|
|Conchocarpus fontanesianus (A. St.-Hill.) Kallunki & Pirani, Rutaceae, popularly known as pitaguará, is a native and endemic tree from São Paulo and Rio de Janeiro States, Brazil. Based in the information that anticholinesterasic derivatives could act as new prototypes to treatment of Alzheimer disease, this work describes the fractionation guided by evaluation of the anticholinesterase activity of the ethanolic stems extract from C. fontanesianus. This procedure afforded the alkaloids Dictamnine (1), γ-fagarine (2), skimianine (3), and 2-phenyl-1-methyl-4-quinolone (4), as well as the coumarin marmesin (5).|
Chinese Journal of Experimental Traditional Medical Formulae, 2012, 18(14):128-31.
|Isolation and Anti-inflammatory Effect of Dictamnine Extracted from Dictamni Cortex.[Reference: WebLink]|
|To detect the anti-inflammatory effects of ethanol extracts and Dictamnine extracted and isolated from Dictamni Cortex.Method:High performance centrifugal partition chromatography method was used for separation of the essential Dictamnine,and anti-inflammatory effects of ethanol extracts and Dictamnine were investigated by xylene-induced aruical swelling in mice and the abdominal capillary permeability in mice.Result:Separation was performed with a two-phase solvent system composed of hexane ethylacetate-ethanol-water(1∶1∶1∶1).The purity of Dictamnine obtained was 98% determined by high performance liquid chromatograph.The results showed that each dosage of ethanol extracts could significantly(P0.05) suppress the mouse auricle which caused by the xylene swelling(being 44.26%,52.46%,44.26%,respectively),as compared with the control group.The medium-dosage group of Dictamnine could obviously(P0.01) suppress the mouse auricle but the dosage was not increased significantly in abdominal capillary permeability in mice compared with control group.Conclusion: HPCPC is a recommendable method to prepare and purify the dictamnie with good separation and the method is simple,accurate and easy to operate.The results showed that ethanol extracts and Dictamnine showed different anti-inflammatory effect in variety of animal models of anti-inflammatory.|
Chem Biol Interact. 1989;72(1-2):105-11.
|Further evidence for photoinduced genotoxicity of dictamnine as shown by prophage induction.[Pubmed: 2684439]|
|Photobiological activity of Dictamnine, a furoquinoline alkaloid, to induce lytic phage development in a lysogen of Escherichia coli was measured as a line of evidence for the photoinduced genotoxicity. Since Dictamnine forms the monoadducts to DNA but not the diadducts (DNA cross-links) by photoirradiation, the photobiological activity was compared with that of a cross-linking agent 5-methoxypsoralen, a structural analog, on the basis of relative quantum yield. The activity of Dictamnine with respect to both phage induction and photoinduced lethal activity was weaker than the psoralen derivative. Any lethal DNA damage including monoadducts and diadducts of 5-methoxypsoralen appeared to contribute to prophage induction at the same level of efficiency.|
Yeast. 2008 Sep;25(9):631-41.
|Global gene expression profile of Saccharomyces cerevisiae induced by dictamnine.[Pubmed: 18727144]|
|Dictamnine, a natural plant product, has been reported to have antimicrobial activity against bacteria and fungi; however, the Dictamnine response mechanisms of microorganisms are still poorly understood. We have shown that Dictamnine has antimicrobial activities against the model fungus Saccharomyces cerevisiae, with a minimum inhibitory concentration (MIC) value of 64 microg/ml. Commercial oligonucleotide microarrays were used to determine the global transcriptional response of S. cerevisiae triggered by treatment with Dictamnine. We interpreted our microarray data using the hierarchical clustering tool, T-profiler. Several major transcriptional responses were induced by Dictamnine. The first was the induced environmental stress response, mainly under the control of the Msn2p and Msn4p transcription factors, and the repressed environmental stress response in genes containing the PAC (RNA polymerase A and C box) and rRPE (ribosomal RNA processing element) motifs. The second was the Upc2p-mediated response involved in lipid biosynthesis. The third comprised the PDR3- and RPN4-mediated responses involved in multidrug resistance (MDR). Finally, the TBP-mediated response was induced with Dictamnine treatment. TBP is an essential general transcription factor involved in directing the transcription of genes. Quantitative real-time RT-PCR was performed on selected genes to verify the microarray results. Furthermore, morphological transitions during Dictamnine exposure to S. cerevisiae L1190 (MATa/alpha) were examined, using confocal laser microscopy.|
Chinese Journal of Pharmacology & Toxicology, 2013, 27(1):95-100.
|Cytotoxic effect of dictamnine on HepG2 cells and its possible mechanism[Reference: WebLink]|
|Dictamnine at higher concentrations(≥100 μmol·L-1)has potential hepatotoxicity.The cell membrane damage and mitochondrial membrane damage may be involved in the Dictamnine-induced hepatotoxity mechanism.|
Chinese Agricultural Science Bulletin, 2009, 25(16): 21-4.
|Study on in Vitro Antifungal Activity of Dictamnine against Candida albicans.[Reference: WebLink]|
|Candida albicans (C. albicans) and other fungi important human and veterinary pathogens. The minimal inhibitory concentration (MIC) of Dictamnine alone and in combination with fluconazole (FLC) against clinical 22 clinical Candida albicans were tested, and checkerboard assay was used to analyze the ractional inhibitory concentration index (FICI) of the combination of Dictamnine and FLC. The result showed that Dictamnine had good antifungal activity alone and in combination with FLC against Candida albicans.|
Mutat Res. 1985 Dec;144(4):221-5.
|Mutagenic activities of dictamnine and gamma-fagarine from dictamni radicis cortex (Rutaceae).[Pubmed: 4069140]|
|A methanol extract of Dictamni Radicis Cortex exhibited a mutagenic effect on Salmonella typhimurium TA100 and TA98 with S9 mix. Two mutagenic compounds in Dictamni Radicis Cortex were isolated on a Sephadex LH 20 column and silica gel column chromatography and by preparative TLC. These were identified as Dictamnine and gamma-fagarine by UV, EI-Mass, 1H-NMR. Dictamnine and gamma-fagarine were mutagenic in strain TA100 and TA98 with S9 mix. The dose-response curves were linear in the range 10-40 micrograms. Dictamnine and gamma-fagarine had specific activities (His+/microgram) of about 50-70 revertant colonies in strain TA100, while in strain TA98 there were about 30-50 revertant colonies.|