|Source:||The fruit body of Ganoderma lucidum|
|Biological Activity or Inhibitors:||1. Ganodermanontriol, a lanostanoid triterpene from Ganoderma lucidum, suppresses growth of colon cancer cells through ß-catenin signaling, it may be a potential chemotherapeutic agent for the treatment of cancer.
2. Ganodermanontriol has hepatoprotective effect against t -BHP-induced oxidative stress in vitro and in vivo , it induces in vitro and in vivo anti-inflammatory activity in t -BHP-damaged hepatic cells through the expression of HO-1, and in which PI3K/Akt and p38 kinases are involved.
3. Ganodermanontriol and ganoderiol F are active as anti-HIV-1 agents with an inhibitory concentration of 7.8 micrograms ml-1 for both.
4. Ganodermanontriol showes antioxidative activities in a dose dependent manner .
5. Ganodermanontriol shows a strong anticomplement activity against the classical pathway (CP) of the complement system with IC(50) values of 17.2 microM.
|Solvent:||Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.|
|Storage:||Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please email to: firstname.lastname@example.org
|After receiving:||The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.|
|1 mg||5 mg||10 mg||20 mg||25 mg|
|1 mM||2.1155 mL||10.5775 mL||21.1551 mL||42.3101 mL||52.8877 mL|
|5 mM||0.4231 mL||2.1155 mL||4.231 mL||8.462 mL||10.5775 mL|
|10 mM||0.2116 mL||1.0578 mL||2.1155 mL||4.231 mL||5.2888 mL|
|50 mM||0.0423 mL||0.2116 mL||0.4231 mL||0.8462 mL||1.0578 mL|
|100 mM||0.0212 mL||0.1058 mL||0.2116 mL||0.4231 mL||0.5289 mL|
J Ethnopharmacol. 2013 Dec 12;150(3):875-85.
|In vitro and in vivo hepatoprotective effect of ganodermanontriol against t-BHP-induced oxidative stress.[Pubmed: 24140584]|
|Ganoderma lucidum (Fr.) Karst. (Ganodermataceae) is a mushroom which is used as a traditional remedy in the treatment of human diseases such as hepatitis, liver disorders, hypercholesterolemia, arthritis, bronchitis and tumorigenic diseases. This study targets the evaluation of hepatoprotective activity of Ganodermanontriol, a sterol isolated from Ganoderma lucidum, and the investigation of its mechanism of action in Hepa1c1c7 and murine liver cells upon tert-butyl hydroperoxide (t-BHP)-induced inflammation. t-BHP was utilized to stimulate an anti-inflammatory reaction in the hepatic cell lines and murine hepatic tissue examined.|
Phytochemistry. 1998 Nov;49(6):1651-7.
|Anti-HIV-1 and anti-HIV-1-protease substances from Ganoderma lucidum.[Pubmed: 9862140]|
|A new highly oxygenated triterpene named ganoderic acid alpha has been isolated from a methanol extract of the fruiting bodies of Ganoderma lucidum together with twelve known compounds. The structures of the isolated compounds were determined by spectroscopic means including 2D-NMR. Ganoderiol F and Ganodermanontriol were found to be active as anti-HIV-1 agents with an inhibitory concentration of 7.8 micrograms ml-1 for both, and ganoderic acid B, ganoderiol B, ganoderic acid C1, 3 beta-5 alpha-dihydroxy-6 beta-methoxyergosta-7,22-diene, ganoderic acid alpha, ganoderic acid H and ganoderiol A were moderately active inhibitors against HIV-1 PR with a 50% inhibitory concentration of 0.17-0.23 mM.|
Int J Oncol. 2011 Mar;38(3):761-7.
|Ganodermanontriol, a lanostanoid triterpene from Ganoderma lucidum, suppresses growth of colon cancer cells through ß-catenin signaling.[Pubmed: 21225227]|
|The medicinal mushroom Ganoderma lucidum (GL) is a rich source of triterpenes with anticancer properties. Here, we show that Ganodermanontriol (GNDT), a purified triterpene from GL, inhibited proliferation of HCT-116 and HT-29 colon cancer cells without a significant effect on cell viability. Moreover, Ganodermanontriol inhibited transcriptional activity of ß-catenin and protein expression of its target gene cyclin D1 in a dose-dependent manner. A marked inhibition effect was also seen on Cdk-4 and PCNA expression, whereas expression of Cdk-2, p21 and cyclin E was not affected by the Ganodermanontriol treatment. In addition, Ganodermanontriol caused a dose-dependent increase in protein expression of E-cadherin and ß-catenin in HT-29 cells. Finally, Ganodermanontriol suppressed tumor growth in a xenograft model of human colon adenocarcinoma cells HT-29 implanted in nude mice without any side-effects and inhibited expression of cyclin D1 in tumors. In conclusion, our data suggest that Ganodermanontriol might be a potential chemotherapeutic agent for the treatment of cancer.|
Phytother. Res., 1999, 13(6):529–31.
|Triterpene antioxidants from ganoderma lucidum.[Pubmed: 10479768]|
|Ganoderma lucidum was studied for its antioxidative activity by bioassay guided isolation in conjunction with in vitro tests. The powdered crude drug was treated with boiling water and the aqueous extract (Ex1) was further separated to obtain terpene and polysaccharide fractions. The two fractions and Ex1 were screened for their antioxidative effect against pyrogallol induced erythrocyte membrane oxidation and Fe (II)-ascorbic acid induced lipid peroxidation. All tested samples showed antioxidative activities in a dose dependent manner and the terpene fraction was found to possess the highest effect compared with the others. Chemical isolation of the terpene fraction resulted in the detection of ganoderic acids A, B, C and D, lucidenic acid B and Ganodermanontriol as major ingredients.|
Planta Med. 2001 Dec;67(9):811-4.
|Anticomplement activity of terpenoids from the spores of Ganoderma lucidum.[Pubmed: 11745016 ]|
|A new lanostane-type terpenoid, lucidenic acid SP1 (1), was isolated from a CHCl(3)-soluble fraction of Ganoderma lucidum spores together with four other known compounds (2 - 5). The structure of lucidenic acid SP1 was determined to be 3 beta,7 beta-dihydroxy-4,4,14 alpha-trimethyl-11,15-dioxo-5 alpha-chol-8-en-24-oic acid by spectroscopic means including 2D-NMR. Twelve triterpenes (1-12) isolated from G. lucidum spores were investigated in vitro for their anticomplementary activity. Compounds 1 - 5 were inactive, whereas ganoderiol F (8), ganodermanondiol (9) and Ganodermanontriol (10) showed a strong anticomplement activity against the classical pathway (CP) of the complement system with IC(50) values of 4.8, 41.7, and 17.2 microM, respectively. The potency of these triterpene alcohols (8-10) in inhibiting CP activity was improved when the number of hydroxymethyl groups on the side chain moiety is increased. On the other hand, the ganoderic acids 1-7, which contain a carboxyl group in the side chain, and lucidumols A and B (11, 12) had little activity on this system.|