|Source:||The roots of Panax ginseng C. A. Mey.|
|Biological Activity or Inhibitors:||1. 20(R)-Protopanaxatriol shows anti-hyperglycaemic activities.
2. Protopanaxatriol has inhibitory effects on the enzyme catalytic activities of cyclooxygenases-1 and -2 (COX-1 and -2).
3. 20(R)-Protopanaxatriol possesses cytotoxicities against three human cancer cell lines HeLa, A549 and MCF-7.
|Solvent:||Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.|
|Storage:||Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please email to: email@example.com
|After receiving:||The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.|
|1 mg||5 mg||10 mg||20 mg||25 mg|
|1 mM||2.0976 mL||10.4881 mL||20.9762 mL||41.9525 mL||52.4406 mL|
|5 mM||0.4195 mL||2.0976 mL||4.1952 mL||8.3905 mL||10.4881 mL|
|10 mM||0.2098 mL||1.0488 mL||2.0976 mL||4.1952 mL||5.2441 mL|
|50 mM||0.042 mL||0.2098 mL||0.4195 mL||0.839 mL||1.0488 mL|
|100 mM||0.021 mL||0.1049 mL||0.2098 mL||0.4195 mL||0.5244 mL|
J. Funct. Foods, 2016, 23:188-97.
|The inhibition of α-glycosidase and protein tyrosine phosphatase 1B (PTP1B) activities by ginsenosides from Panax ginseng C.A. Meyer and simultaneous determination by HPLC-ELSD.[Reference: WebLink]|
|Panax ginseng C.A. Meyer is extensively used as a food additive because of its medicinal and nutritional properties. This study investigated the inhibitory activity of eight ginsenosides against α-glycosidase and protein tyrosine phosphatase 1B (PTP1B). Results showed the anti-hyperglycaemic activities of the eight ginsenosides were in the order of 20(R)-dammarane-3β, 6α, 12β, 20, 25-pentol (25-OH-PPT) > 20(R)-25-methoxydammarane-3β, 12β, 20-tetrol (25-OCH3-PPT) > 20(R)-Protopanaxatriol (PPT) > 20(S)-panaxatriol (PT) > 20(R)-dammarane-3β, 12β, 20, 25-tetrol (25-OH-PPD) > 20(R)-25-methoxydammarane-3β, 12β, 20-triol (25-OCH3-PPD) > 20(R)-protopanaxadiol (PPD) > 20(S)-panaxadiol (PD), and 25-OH-PPT exerted stronger inhibitory activity than acarbose and Na3VO4. Meanwhile, simultaneous quantitative estimation results of eight ginsenosides suggested good linear regression within test ranges, precision, accuracy. 25-OH-PPT of the stems (leaves), flowers, and fruits contained 2.69, 3.31 and 7.20%, respectively. High performance liquid chromatography-evaporative light-scattering detector (HPLC-ELSD) method was proven to be simple, fast, and effective.|
Food Chem., 2012, 133(3):998-1000.
|Cyclooxygenase inhibitory activity of ginsenosides from heat-processed ginseng.[Reference: WebLink]|
|Ginsenosides, from heat-processed ginseng, and sapogenins were evaluated for their inhibitory effects on the enzyme catalytic activities of cyclooxygenases-1 and -2 (COX-1 and -2). The ginsenosides, 20(S)-Rg3, Rg5, and Rk1, inhibited COX-2 activity, but did not affect the enzyme activity of COX-1. Protopanaxatriol (PPT) moderately inhibited both COX-1 and -2. The ginsenosides, 20(R)-Rg3, Re, and protopanaxadiol (PPD) showed a minimal effect on COX-1 and -2 activities. Taken together, ginsenosides Rg3 (20S-form), Rg5 and Rk1 showed selective inhibitory activity on COX-2 by behaving as an inhibitor of the enzyme–substrate reaction.|
Biomed Chromatogr. 2011 Jun;25(6):646-51.
|Simultaneous determination of five active hydrolysis ingredients from Panax quinquefolium L. by HPLC-ELSD.[Pubmed: 20737654]|
|An effective method for simultaneous determination of five hydrolysis products of 20 (R)-dammarane-3β,6α,12β,20,25-pentol, 24(R)-ocotillol, 20(R)-Protopanaxatriol, 20(S)-panaxatriol and 20(R)-dammarane-3β,12β,20,25-tetrol was developed using high-performance liquid chromatography with evaporative light scattering detection (HPLC-ELSD). The hydrolysis products from Panax quinquefolium L. in the stems and leaves, berries, flower buds and roots components were successfully separated on a Kromasil C(18) column using methanol and water (83:17, v/v) as mobile phase in 18 min. The parameter for the ELSD was set to a probe temperature of 40°C and the nebulizer for nitrogen gas was adjusted to 3 L/min. All calibration curves showed good linear regression (r > 0.9975) within test ranges. The validation of the method included recovery, linearity, accuracy and precision (intra- and inter-day variation). The accuracy and precision were satisfactory, with the overall intra- and inter-day variation being less than 3.11%, and recoveries of this method were greater than 95.0%. This study developed an effective and rapid method for simultaneous determination of multiple hydrolysis components from Panax quinquefolium L.|
Chemical Research in Chinese Universities, 2016, 32(1): 1-6.
|Semisynthesis and cytotoxicity evaluation of a series of ocotillol type saponins and aglycones from 20(S)-ginsenoside Rg2, Rh1, protopanaxatriol and their 20(R)-epimers[Reference: WebLink]|
|With the oxidation treatment, eighteen compounds were separated from 20(S)-ginsenoside Rg2, Rh1, protopanaxatriol(PPT) and their 20(R)-epimers in total and cytotoxicity of most of them was evaluated against three human cancer cell lines HeLa, A549 and MCF-7 by 3-(4,5-dimetylthiazol-z-yl)-2,5-diphenyltetrazolium bromide(MTT) assay. Their structures were confirmed by means of nuclear magnetic resonance(NMR) and mass spectrometry and the results were compared with those of previous literature. In this study, we systematically semisynthesized all four ocotillol type saponins, i.e., (20S, 24S), (20S, 24R), (20R, 24S) and (20R, 24R). All the configurations at C20 kept the same with their starting materials. Meanwhile a pair of C24 epimers was generated in terms of ocotillol type saponins. In addition, seven compounds(4―8, 13 and 14) were reported firstly. The cytotoxic results distinguished the ocotillol type products(6, 7, 13 and 14) from 20(R)-Protopanaxatriol and 20(R)-ginsenoside Rh1, which possessed better cytotoxicities than their correspondents from 20(S)-epimers against HeLa cells, and the carbonyl group at C3 can improve the cytotoxicity, which helped us to gain deeper insight into Ocotillol type saponins.|