|Source:||The leaves of Stevia rebaudiana Bertoni.|
|Biological Activity or Inhibitors:||1. Rebaudioside A is approximately 250 to 300 times sweeter than sucrose.
2. High purity rebaudioside A is safe for human consumption under its intended conditions of use as a general purpose sweetener.
3. Rebaudioside A do not contribute calories or carbohydrates to the diet and do not affect blood glucose or insulin response.
|Solvent:||Pyridine, Methanol, Ethanol, etc.|
|Storage:||Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please email to: firstname.lastname@example.org
|After receiving:||The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.|
|1 mg||5 mg||10 mg||20 mg||25 mg|
|1 mM||1.0341 mL||5.1706 mL||10.3412 mL||20.6823 mL||25.8529 mL|
|5 mM||0.2068 mL||1.0341 mL||2.0682 mL||4.1365 mL||5.1706 mL|
|10 mM||0.1034 mL||0.5171 mL||1.0341 mL||2.0682 mL||2.5853 mL|
|50 mM||0.0207 mL||0.1034 mL||0.2068 mL||0.4136 mL||0.5171 mL|
|100 mM||0.0103 mL||0.0517 mL||0.1034 mL||0.2068 mL||0.2585 mL|
Molecules. 2014 Oct 28;19(11):17345-55.
|Bioconversion of rebaudioside I from rebaudioside A.[Pubmed: 25353385]|
|To supply the increasing demand of natural high potency sweeteners to reduce the calories in food and beverages, we have looked to steviol glycosides. In this work we report the bioconversion of Rebaudioside A to rebaudioside I using a glucosyltransferase enzyme. This bioconversion reaction adds one sugar unit with a 1→3 linkage. We utilized 1D and 2D NMR spectroscopy (1H, 13C, COSY, HSQC-DEPT, HMBC, 1D TOCSY and NOESY) and mass spectral data to fully characterize rebaudioside I.|
Food Chem Toxicol. 2014 Apr;66:334-40.
|The non-cytotoxicity characterization of rebaudioside A as a food additive.[Pubmed: 24500608]|
|To evaluate the cytotoxicity of high-purity Rebaudioside A (reb A, 99.16%) as a food ingredient, a combination of several methods, including tetrazolium-based colorimetric assay (MTT), lactate dehydrogenase assay (LDH), enzyme-linked immunosorbent assay (ELISA), real-time PCR (qPCR), high-performance liquid chromatography (HPLC), and two-dimensional electrophoresis (2-DE) were used to test the cytotoxicity of Rebaudioside A on the human cells HT-29 and T84, as well as liver and spleen cells from mice. The results indicated that no obvious changes in cellular viability, inflammatory cytokines yield, or protein yield were observed between the test group and the control group when different concentrations of Rebaudioside A were used, suggesting that Rebaudioside A is non-cytotoxic in vitro at the concentrations range tested (0.001-0.5%).|
Carbohydr Res. 2009 Dec 14;344(18):2533-8.
|NMR studies of the conformation of the natural sweetener rebaudioside A.[Pubmed: 19889398]|
|Rebaudioside A is a natural sweetener from Stevia rebaudiana in which four beta-D-glucopyranose units are attached to the aglycone steviol. Its (1)H and (13)C NMR spectra in pyridine-d(5) were assigned using 1D and 2D methods. Constrained molecular dynamics of solvated rebaudioside using NMR constraints derived from ROESY cross peaks yielded the orientation of the beta-D-glucopyranose units. Hydrogen bonding was examined using the temperature coefficients of the hydroxyl chemical shifts, ROESY and long-range COSY spectra, and proton-proton coupling constants.|