|Source:||The seeds of Psoralea grandulosa|
|Biological Activity or Inhibitors:||1. Angelicin may provide a lead structure for the development of antiviral drug against gammaherpesviruses.
2. Angelicin might be a potential new agent for prevention of inflammatory reactions and diseases in the clinic.
3. Angelicin is an effective apoptosis-inducing natural compound of human SH-SY5Y neuroblastoma cells.
4. Angelicin, at high seed productivity of H. sosnowskyi may have an ecological significance in plant-plant interaction.
|Solvent:||Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.|
|Storage:||Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please email to: firstname.lastname@example.org
|After receiving:||The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.|
|1 mg||5 mg||10 mg||20 mg||25 mg|
|1 mM||5.3706 mL||26.8528 mL||53.7057 mL||107.4114 mL||134.2642 mL|
|5 mM||1.0741 mL||5.3706 mL||10.7411 mL||21.4823 mL||26.8528 mL|
|10 mM||0.5371 mL||2.6853 mL||5.3706 mL||10.7411 mL||13.4264 mL|
|50 mM||0.1074 mL||0.5371 mL||1.0741 mL||2.1482 mL||2.6853 mL|
|100 mM||0.0537 mL||0.2685 mL||0.5371 mL||1.0741 mL||1.3426 mL|
J Surg Res. 2013 Nov;185(1):300-9.
|Angelicin regulates LPS-induced inflammation via inhibiting MAPK/NF-κB pathways.[Pubmed: 23816246]|
|BACKGROUND: Angelicin is a furocoumarin found in Psoralea corylifolia L. fruit. The purpose of this study was to investigate the protective ability of Angelicin against inflammation in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells and LPS-induced in vivo acute lung injury model. RESULTS: The results showed that pretreatment with Angelicin markedly downregulated TNF-α and IL-6 levels in vitro and in vivo, and significantly decreased the amount of inflammatory cells, lung wet-to-dry weight ratio, and myeloperoxidase activity in LPS-induced ALI mice. Furthermore, Western blotting analysis results demonstrated that Angelicin blocked the phosphorylation of IκBα, NF-κBp65, p38 MAPK, and JNK in LPS-induced ALI. CONCLUSIONS: These results suggest that Angelicin was potentially advantageous to prevent inflammatory diseases by inhibiting NF-κB and MAPK pathways. Our data indicated that Angelicin might be a potential new agent for prevention of inflammatory reactions and diseases in the clinic.|
Antiviral Res. 2013 Oct;100(1):75-83.
|Antiviral activity of angelicin against gammaherpesviruses.[Pubmed: 23892155]|
|We identified the antiviral activity of Angelicin against murine gammaherpesvirus 68 (MHV-68), genetically and biologically related to human gammaherpesviruses. Angelicin, a furocoumarin naturally occurring tricyclic aromatic compound, efficiently inhibited lytic replication of MHV-68 in a dose-dependent manner following the virus entry. The IC50 of Angelicin antiviral activity was estimated to be 28.95μM, while the CC50 of Angelicin was higher than 2600μM. Furthermore, incubation with Angelicin efficiently inhibited 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced lytic replication of human gammaherpresviruses in both EBV- and KSHV-infected cells. Taken together, these results suggest that MHV-68 can be a useful tool to screen novel antiviral agents against human gammaherepsviruses and that Angelicin may provide a lead structure for the development of antiviral drug against gammaherpesviruses.|
Nat Prod Commun. 2015 May;10(5):767-70.
|Angelicin as the principal allelochemical in Heracleum sosnowskyi fruit.[Pubmed: 26058154]|
|The inhibitory activity of Angelicin was proved to be the highest compared with the other tested furocoumarins, and the inhibitory activity of crude extracts could be explained mainly by the presence of Angelicin. Both, monocots (Lolium multiflorum, Phleum pratensis, Festuca pratesis, Lolium perenne) and dicots (Tripholium repens, Trifolium pretense) were found to be sensitive to the exudates of whole H. sosnowskyi fruits. Thus, we assume, that high inhibitory potential of furocoumarins, especially Angelicin, at high seed productivity of H. sosnowskyi might have an ecological significance in plant-plant interaction.|
Mol Cell Biochem. 2012 Oct;369(1-2):95-104.
|Angelicin induces apoptosis through intrinsic caspase-dependent pathway in human SH-SY5Y neuroblastoma cells.[Pubmed: 22766766]|
|Angelicin is structurally related to psoralens, a well-known chemical class of photosensitizers used for its antiproliferative activity in treatment of different skin diseases. To verify the activity of Angelicin, we employed human SH-SY5Y neuroblastoma cells to investigate its cytotoxicity, although its mechanism of action has not yet been fully elucidated. Here, we examined the cellular cytotoxicity of Angelicin by cell viability assay, DNA fragmentation by DNA ladder assay, and activation of caspases and Bcl-2 family proteins by western blot analyses. The results of our investigation suggest that Angelicin increased cellular cytotoxicity in a dose- and time-dependent manner with IC(50) of 49.56 μM at 48 h of incubation. In addition, Angelicin dose-dependently downregulated the expression of anti-apoptotic proteins including Bcl-2, Bcl-xL, and Mcl-1 suggesting the involvement of the intrinsic mitochondria-mediated apoptotic pathway which did not participate in Fas/FasL-induced caspase-8-mediated extrinsic, MAP kinases, and PI3K/AKT/GSK-3β pathway. Furthermore, we clarified the dose-dependent upregulation of caspase-9 and caspase-3 which indicated that Angelicin-induced apoptosis is mediated primarily through the intrinsic caspase-mediated pathway. In particular, the caspase-3 inhibitor, DEVD-fmk, induced a reduction in Angelicin-induced cytotoxicity which confirmed that the intrinsic caspase-dependent pathway during this apoptosis which did not prevent cytotoxicity using MAP kinases and GSK-3 inhibitor. Taken together, our data shows that Angelicin is an effective apoptosis-inducing natural compound of human SH-SY5Y neuroblastoma cells which suggests that this compound may have a role in future therapies for human neuroblastoma cancer.|