|Source:||The herbs of Catha edulis|
|Biological Activity or Inhibitors:||1. Cathinone generates oxidative stress hampered antioxidant enzymes, glutathione and lipid peroxidation.
2. Cathinone induces significant behavioral changes and CNS activation in the hamster by systemic administration .
3. Cathinone causes hormonal alterations probably via changes in hypothalamo-hypophyseo-adrenocortical and gonadal axes integrity.
|Solvent:||Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.|
|Storage:||Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please email to: firstname.lastname@example.org
|After receiving:||The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.|
|1 mg||5 mg||10 mg||20 mg||25 mg|
|1 mM||6.7029 mL||33.5143 mL||67.0286 mL||134.0572 mL||167.5716 mL|
|5 mM||1.3406 mL||6.7029 mL||13.4057 mL||26.8114 mL||33.5143 mL|
|10 mM||0.6703 mL||3.3514 mL||6.7029 mL||13.4057 mL||16.7572 mL|
|50 mM||0.1341 mL||0.6703 mL||1.3406 mL||2.6811 mL||3.3514 mL|
|100 mM||0.067 mL||0.3351 mL||0.6703 mL||1.3406 mL||1.6757 mL|
Eur J Pharmacol. 2015 Jul 5;758:142-6.
|Direct and indirect cardiovascular actions of cathinone and MDMA in the anaesthetized rat.[Pubmed: 25863258]|
|Effects of Cathinone or MDMA were compared with those of the indirect sympathomimetic tyramine. Male Wistar rats were anaesthetized with pentobarbitone for blood pressure and heart rate recording. Some rats were sympathectomised by treatment with 6-hydroxydopamine. In the anaesthetised rat, Cathinone, MDMA and tyramine (all 0.001-1 mg/kg) produced marked tachycardia, tyramine produced marked pressor responses and MDMA produced small pressor responses. The tachycardia to Cathinone and MDMA was almost abolished by propranolol (1mg/kg). Pretreatment with cocaine (1mg/kg) did not significantly affect the tachycardia to Cathinone or MDMA, but reduced the response to tyramine. However, in sympathectomised rats, the tachycardia to Cathinone or MDMA was markedly attenuated, but the tachycardia to tyramine was only partially reduced. The use of chemical sympathectomy achieved the desired goal of demonstrating that cardiac β-adrenoceptor mediated actions of Cathinone and MDMA are probably largely indirect.|
J Ethnopharmacol. 2014 Oct 28;156:102-6.
|Cathinone, an active principle of Catha edulis, accelerates oxidative stress in the limbic area of swiss albino mice.[Pubmed: 25153022]|
|ETHNOPHARMACOLOGICAL RELEVANCE: Cathinone hydrochloride is an active principle of the khat plant (Catha edulis) that produces pleasurable and stimulating effects in khat chewers. To the best of our knowledge no data of Cathinone on oxidative stress in limbic areas of mice is available. This is the first study of Cathinone on oxidative stress in limbic areas of the brain in Swiss albino male mice. RESULTS: The level of lipid peroxidation (LPO) was elevated dose-dependently and was significant (p<0.05, p<0.01) with doses of 0.25 and 0.5mg/kg body wt. of Cathinone as compared to control group. In contrast, the content of reduced glutathione (GSH) was decreased significantly (p<0.01, p<0.001) with doses of 0.25 and 0.5mg/kg body wt. of Cathinone as compared to control group. The activity of antioxidant enzymes (GPx, GR, GST, CAT, and SOD) was also decreased dose-dependently: the decreased activity of GPx, GR, catalase and SOD was significant with doses of 0.25 and 0.5 mg of Cathinone as compared to control group, while the activity of GST was decreased dose-dependently and was significant with 0.5mg of Cathinone as compared to control group. CONCLUSIONS: The results indicate that the Cathinone generated oxidative stress hampered antioxidant enzymes, glutathione and lipid peroxidation.|
Neurosci Lett. 2014 Jan 24;559:34-8.
|Cathinone increases body temperature, enhances locomotor activity, and induces striatal c-fos expression in the Siberian hamster.[Pubmed: 24287379]|
|Cathinone is a β-keto alkaloid that is the major active constituent of khat, the leaf of the Catha edulis plant that is chewed recreationally in East Africa and the Middle East. Related compounds, such as methCathinone and mephedrone have been increasing in popularity as recreational drugs, resulting in the recent proposal to classify khat as a Class C drug in the UK. There is still limited knowledge of the pharmacological effects of Cathinone. This study examined the acute effects of Cathinone on core body temperature, locomotor and other behaviors, and neuronal activity in Siberian hamsters. Adult male hamsters, previously implanted with radio telemetry devices, were treated with Cathinone (2 or 5mg/kg i.p.), the behavioral profile scored and core body temperature and locomotor activity recorded by radio telemetry. At the end of the study, hamsters received vehicle or Cathinone (5mg/kg) and neuronal activation in the brain was determined using immunohistochemical evaluation of c-fos expression. Cathinone dose-dependently induced significant (p<0.0001) increases in both temperature and locomotor activity lasting 60-90min. Cathinone (2mg/kg) increased rearing (p<0.02), and 5mg/kg increased both rearing (p<0.001) and lateral head twitches (p<0.02). Both Cathinone doses decreased the time spent at rest (p<0.001). The number of c-fos immunopositive cells were significantly increased in the striatum (p<0.0001) and suprachiasmatic nucleus (p<0.05) following Cathinone, indicating increased neuronal activity. There was no effect of Cathinone on food intake or body weight. It is concluded that systemic administration of Cathinone induces significant behavioral changes and CNS activation in the hamster.|
Metab Brain Dis. 2014 Jun;29(2):451-8.
|Dose-response inhibitory effects of purified cathinone from khat (Catha edulis) on cortisol and prolactin release in vervet monkeys (Chlorocebus aethiops).[Pubmed: 24190428]|
|This study reports acute and sub-chronic effects of Cathinone on hormonal alterations in single-caged vervet monkeys. Fourteen adult vervet monkeys were used, 12 of which were treated and 2 controls. Pre-treatment phase of 1 month aimed at establishing baseline levels of hormones while treatment phase of 4 months considered the dose- and time-response effects of Cathinone on serum cortisol and prolactin levels. Test animals were allocated four groups of three animals each and administered 0.8, 1.6, 3.2 and 6.4 mg/kg body weight of Cathinone orally while controls were administered normal saline. Dependent effect of Cathinone on cortisol levels with a significant dose by week interaction [F (71, 142) = 4.86, P < 0.001]. Similarly, there was a decrease in serum prolactin [F (4, 8) = 267, P < 0.001] with escalating doses of Cathinone with a significant dose x week interaction [F (59, 118) = 13.03, P < 0.001]. The findings demonstrate that at high doses and long-term exposure, Cathinone causes hormonal alterations probably via changes in hypothalamo-hypophyseo-adrenocortical and gonadal axes integrity.|
Drug Test Anal. 2014 Jul-Aug;6(7-8):716-27.
|Cross-reactivity of designer drugs, including cathinone derivatives, in commercial enzyme-linked immunosorbent assays.[Pubmed: 23677923]|
|In this experiment, 16 different ELISA reagents were evaluated to determine the cross-reactivity of 30 designer drugs, including 24 phenylethylamines (including 8 Cathinone derivatives), 3 piperazines, and 3 tryptamines. Cross-reactivity towards most drugs was <4% in assays targeting amphetamine or methamphetamine. Compounds such as MDA, MDMA, ethylamphetamine, and α-methyltryptamine demonstrated cross-reactivities in the range of 30-250%, but data were consistent with both manufacturer's inserts and published literature. When tested against the Randox Mephedrone/MethCathinone kit, Cathinone derivatives demonstrated cross-reactivity at concentrations as low as 150 ng/ml.|