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More articles cited ChemFaces products.
LWT - Food Science and Technology2017 Jan.Clin Exp Pharmacol Physiol.2015 Aug 1Phytomedicine1 Feb. 2018;Phytother Res.2018 Jan 29.Food Chem.2018 Jun 30;
BMC Complement Altern Med.2017 Aug 9;Biochem Systematics and EcologyOctober 2017;J Ethnopharmacol. 2017 Jan 20;Cell Physiol Biochem. 2017;44(4);Naunyn Schmiedebergs Arch Pharmacol. 2017 Jul 21.
Int J Anal Chem.2017;Br J Pharmacol.2018 Mar;Phytochemistry Letters22 Dec. 2016Nat Plants. 2016 Dec 22
Our products had been exported to the following research institutions and universities, And still growing.
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||Beta-Sitosterol is a novel plant-derived angiogenic factor which may have potential pharmaceutical applications for the management of chronic wounds. It has potent anti-inflammatory, anti-proliferation, and pro- apoptosis activities, it also possesses antipyretic activity, similar to acetylsalicylic acid. |
||PARP | Bcl-2/Bax | Caspase | IAP|
|Biol Pharm Bull. 2007 Jul;30(7):1317-23. |
|Beta-sitosterol induces anti-proliferation and apoptosis in human leukemic U937 cells through activation of caspase-3 and induction of Bax/Bcl-2 ratio.[Pubmed: 17603173]|
|Beta-Sitosterol is the main dietary phytosterol found in plants and has been shown to inhibit proliferation and induce apoptosis in human solid tumors such as colon and breast cancers. However, the mechanism by which Beta-Sitosterol induces apoptosis is not completely understood in leukemic cells.
METHODS AND RESULTS:
This study investigated the mechanism of apoptosis induced by Beta-Sitosterol in human leukemic U937 cells. Beta-Sitosterol induced cytotoxicity and apoptosis in U937 cells in a concentration dependent manner, as measured by hemocytometer counts, fluorescence microscopy, agarose gel electrophoresis, and flow cytometry analysis. The increase in apoptosis induced by Beta-Sitosterol was associated with down-regulation of Bcl-2, degradation of poly-(ADP-ribose) polymerase (PARP) and phospholipase C (PLC)-gamma1 protein, and activation of caspase-3. Beta-Sitosterol induced apoptosis was not associated with changes in the expression of Bcl-xL, Bax, or inhibitor of apoptosis proteins (IAPs). z-DEVD-fmk, a caspase-3 specific inhibitor, blocked caspase-3 activation and PARP degradation, and significantly attenuated Beta-Sitosterol-induced apoptosis. This suggests that caspase-3 activation is partially essential for Beta-Sitosterol-induced apoptosis. Bcl-2 overexpression also significantly blocked caspase-3 activation and the decrease in PARP cleavage by Beta-Sitosterol, and effectively attenuated the apoptotic response to Beta-Sitosterol.
These results show that Beta-Sitosterol potently induces apoptosis in U937 cells and that Beta-Sitosterol-induced apoptosis is related to the selective activation of caspase-3 and induction of Bax/Bcl-2 ratio.
|Angiogenesis. 1999;3(2):117-23. |
|A novel angiogenic factor derived from Aloe vera gel: beta-sitosterol, a plant sterol.[Pubmed: 14517429]|
|Aloe vera gel has a beneficial effect on wound healing. Because angiogenesis is an essential process in wound healing, we hypothesized that Aloe vera gel might contain potent angiogenic compounds.
METHODS AND RESULTS:
Here we demonstrate that Aloe vera gel and its extracts are angiogenic on the chorioallantoic membrane (CAM) of chick embryo. Out of the three compounds purified from the final fraction of Aloe vera gel, Beta-Sitosterol showed a potent angiogenic activity in the CAM assay. In the presence of heparin, Beta-Sitosterol stimulated neovascularization in the mouse Matrigel plug assay and the motility of human umbilical vein endothelial cells in an in vitro wound migration assay.
Thus Beta-Sitosterol is a novel plant-derived angiogenic factor which may have potential pharmaceutical applications for the management of chronic wounds.
|Planta Med. 1980 Jun;39(2):157-63. |
|Anti-inflammatory and antipyretic activities of beta-sitosterol[Reference: WebLink]|
METHODS AND RESULTS:
The anti-inflammatory and antipyretic activities of Beta-Sitosterol , isolated from the plant CYPERUS ROTUNDUS has been studied, employing carrageenin induced oedema, cotton pellet implantation and Brewer's yeast induced pyrexia in rats. Beta-Sitosterol was found to possess potent anti-inflammatory activity against both the tests, similar to hydrocortisone and oxyphenbutazone when administered intraperitoneally. Moreover, it was also orally effective against carrageenin induced oedema. The antiinflammatory activity of Beta-Sitosterol was independent of pituitary adrenal axis. Beta-Sitosterol also possessed antipyretic activity, similar to acetylsalicylic acid. However, it was devoid of analgesic activity against aconitine induced writhing in mice. Beta-Sitosterol showed a wide margin of safety since the approximate LD 50 was more than 3 g/kg i.p. in mice and minimum ulcerogenic dose was 600 mg/kg i.p. in rats.
The presence of the anti-inflammatory and antipyretic activities with wide margin of safety, Beta-Sitosterol may be of therapeutic value.
||The herbs of Anemone cathayensis Kitag.
||Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.
||Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please email to: email@example.com
||The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
Recent ChemFaces New Products and Compounds
Recently, ChemFaces products have been cited in many studies from excellent and top scientific journals
Cell. 2018 Jan 11;172(1-2):249-261.e12. doi: 10.1016/j.cell.2017.12.019.PMID: 29328914
Mol Cell. 2017 Nov 16;68(4):673-685.e6. doi: 10.1016/j.molcel.2017.10.022.PMID: 29149595
Scientific Reports 2017 Dec 11;7(1):17332.doi: 10.1038/s41598-017-17427-6.PMID: 29230013
Molecules. 2017 Oct 27;22(11). pii: E1829.doi: 10.3390/molecules22111829.PMID: 29077044
J Cell Biochem. 2018 Feb;119(2):2231-2239.doi: 10.1002/jcb.26385. PMID: 28857247
Phytomedicine. 2018 Feb 1;40:37-47. doi: 10.1016/j.phymed.2017.12.030.PMID: 29496173
Calculate Dilution Ratios(Only for Reference)
* Note: If you are in the process of experiment, it's need to make the dilution ratios of the samples. The dilution data of the sheet for your reference. Normally, it's can get a better solubility within lower of Concentrations.
|Int J Oncol. 2003 Dec;23(6):1657-62. |
|Induction of Bax and activation of caspases during beta-sitosterol-mediated apoptosis in human colon cancer cells.[Pubmed: 14612938]|
|Beta-Sitosterol, a main dietary phytosterol found in plants, may have the potential for prevention and therapy for human cancer.
METHODS AND RESULTS:
The purpose of the present study was to examine the effect of Beta-Sitosterol on the growth of HT116 human colon cancer cells. Treatment with Beta-Sitosterol resulted in a dose-dependent growth inhibition coupled with the characteristic morphological features of apoptosis and with the increase of a sub-G1 cell population. Apoptosis-inducing concentrations of Beta-Sitosterol induced caspase-3 and caspase-9 activation accompanied by proteolytic cleavage of poly(ADP-ribose)-polymerase. In addition, Beta-Sitosterol-induced apoptosis in HT116 cells was associated with a decreased expression of the anti-apototic Bcl-2 protein and mRNA and a concomitant increase of the pro-apototic Bax protein and mRNA, and with release of cytochrome c from the mitochondria into the cytosol. Beta-Sitosterol treatment also inhibited the expression of cIAP-1 without significant changes in the level of cIAP-2.
Taken together, these findings provide important new insights into the possible molecular mechanisms of the anti-cancer activity of Beta-Sitosterol.