|Source:||The flower buds of Chimonanthus praecox.|
|Biological Activity or Inhibitors:||1. (-)-Chimonanthine (IC50 = 0.93 uM) shows potent inhibitory effects on melanogenesis in theophylline-stimulated murine B16 melanoma 4A5 cells.
|Solvent:||Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.|
|Storage:||Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
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|After receiving:||The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.|
|1 mg||5 mg||10 mg||20 mg||25 mg|
|1 mM||2.8862 mL||14.4308 mL||28.8617 mL||57.7234 mL||72.1542 mL|
|5 mM||0.5772 mL||2.8862 mL||5.7723 mL||11.5447 mL||14.4308 mL|
|10 mM||0.2886 mL||1.4431 mL||2.8862 mL||5.7723 mL||7.2154 mL|
|50 mM||0.0577 mL||0.2886 mL||0.5772 mL||1.1545 mL||1.4431 mL|
|100 mM||0.0289 mL||0.1443 mL||0.2886 mL||0.5772 mL||0.7215 mL|
J Nat Med. 2014 Jul;68(3):539-49.
|Dimeric pyrrolidinoindoline-type alkaloids with melanogenesis inhibitory activity in flower buds of Chimonanthus praecox.[Pubmed: 24668298]|
|A methanol extract of the flower buds of Chimonanthus praecox (L.) Link (Calycanthaceae) demonstrated inhibitory effects on melanogenesis in theophylline-stimulated murine B16 melanoma 4A5 cells. From the extract, five dimeric pyrrolidinoindoline alkaloids and four sesquiterpenes were isolated, together with 16 known compounds. Among them, (-)-Chimonanthine (1, IC50 = 0.93 μM), (-)-folicanthine (2, 1.4 μM), and (-)-calycanthidine (3, 1.8 μM) showed potent inhibitory effects without notable cytotoxicity at the effective concentrations. The most potent alkaloid (1) inhibited both tyrosinase and tyrosine-related protein-1 mRNA expressions, to which the melanogenesis inhibitory activity would be ascribable.|