|Source:||The bark of Eucommia ulmoides Oliv.|
|Biological Activity or Inhibitors:||1. Chlorogenic acid, a phenolic compound found ubiquitously in plants, is an in vitro antioxidant and metal chelator, some derivatives of chlorogenic acid are hypoglycemic agents and may affect lipid metabolism;in vivo, chlorogenic acid can improve glucose tolerance, decrease some plasma and liver lipids, and improve mineral pool distribution.
2. Chlorogenic acid and caffeic acid have antioxidant property, have effects on intestinal ischemia–reperfusion injury.
3. Chlorogenic acid has antiviral activity, reduces serum DHBV level in DHBV-infected duckling model, shows inhibitory effect on HBV replication.
4. Chlorogenic acid inhibits the inflammatory reaction in HSE via the suppression of TLR2/TLR9-Myd88 signaling pathways.
|Solvent:||Pyridine, Methanol, Ethanol, etc.|
|Storage:||Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please email to: firstname.lastname@example.org
|After receiving:||The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.|
|1 mg||5 mg||10 mg||20 mg||25 mg|
|1 mM||2.8224 mL||14.1119 mL||28.2239 mL||56.4477 mL||70.5597 mL|
|5 mM||0.5645 mL||2.8224 mL||5.6448 mL||11.2895 mL||14.1119 mL|
|10 mM||0.2822 mL||1.4112 mL||2.8224 mL||5.6448 mL||7.056 mL|
|50 mM||0.0564 mL||0.2822 mL||0.5645 mL||1.129 mL||1.4112 mL|
|100 mM||0.0282 mL||0.1411 mL||0.2822 mL||0.5645 mL||0.7056 mL|
Life Sci. 2015 Mar 2. pii: S0024-3205(15)00105-8.
|Involvement of TLR2 and TLR9 in the anti-inflammatory effects of chlorogenic acid in HSV-1-infected microglia.[Pubmed: 25744394]|
|Chlorogenic acid inhibits the inflammatory reaction in HSE via the suppression of TLR2/TLR9-Myd88 signaling pathways. Chlorogenic acid may serve as an anti-inflammatory agent and provide a new strategy for treating HSE.|
Anticancer Drugs. 2015 Feb 24.
|Chlorogenic acid enhances the effects of 5-fluorouracil in human hepatocellular carcinoma cells through the inhibition of extracellular signal-regulated kinases.[Pubmed: 25714249]|
|Combined with our results, this indicates that the combination of 5-fluorouracil and Chlorogenic acid leads to the inactivation of ERK through the overproduction of ROS. This mediates the enhancement of 5-fluorouracil-induced inhibition of hepatocellular carcinoma cells' proliferation, that is, Chlorogenic acid sensitizes hepatocellular carcinoma cells to 5-fluorouracil treatment by the suppression of ERK activation through the overproduction of ROS. Chlorogenic acid has shown potential as a chemosensitizer of 5-fluorouracil chemotherapy in hepatocellular carcinoma.|
Int J Clin Exp Pathol. 2014 Dec 1;7(12):8797-801.
|Chlorogenic acid suppresses interleukin-1β-induced inflammatory mediators in human chondrocytes.[Pubmed: 25674248]|
|We investigated the anti-inflammatory properties of Chlorogenic acid (CGA) in interleukin-1β-induced chondrocytes. The nitric oxide (NO) and prostaglandin E2 (PGE2) were detected by Griess and Enzyme-linked immunosorbent assay (ELISA) respectively. Quantitative real-time PCR and western blot were performed to measure the expression of inducible NO synthase (iNOS) and cyclooxygenase (COX)-2. Our results indicate that Chlorogenic acid inhibited the production of NO and PGE2 as well as the expression of iNOS and COX-2 in chondrocytes. Our data suggest that Chlorogenic acid possess potential value in the treatment of OA.|
Int J Pharm. 2011 Jan 17;403(1-2):136-8.
|In vitro and in vivo antioxidant properties of chlorogenic acid and caffeic acid.[Pubmed: 20933071 ]|
|Dietary polyphenols are thought to be beneficial for human health as antioxidants. Coffee beans contain a common polyphenol, Chlorogenic acid. Chlorogenic acid is the ester of caffeic acid and quinic acid. We carried out in vitro and in vivo experiments. In the in vitro study, caffeic acid had stronger antioxidant activity than that of Chlorogenic acid. The uptake of Chlorogenic acid by Caco-2 cells was much less than that of caffeic acid. The physiological importance of an orally administered compound depends on its availability for intestinal absorption and subsequent interaction with target tissues. We then used an intestinal ischemia-reperfusion model to evaluate antioxidant activities in vivo. We found that both Chlorogenic acid and caffeic acid had effects on intestinal ischemia-reperfusion injury. Since caffeic acid has a stronger antioxidant activity than that of Chlorogenic acid and Chlorogenic acid is hydrolyzed into caffeic acid in the intestine, it is possible that caffeic acid plays a major role in the protective effect of Chlorogenic acid against ischemia-reperfusion injury.|
J Nutr Biochem. 2002 Dec;13(12):717-726.
|Chlorogenic acid modifies plasma and liver concentrations of: cholesterol, triacylglycerol, and minerals in (fa/fa) Zucker rats.[Pubmed: 12550056]|
|Chlorogenic acid, a phenolic compound found ubiquitously in plants, is an in vitro antioxidant and metal chelator. Some derivatives of Chlorogenic acid are hypoglycemic agents and may affect lipid metabolism. Concentrations of cholesterol and triacylglycerols are of interest due to their association with diseases such as non-insulin-dependent-diabetes- mellitus and obese insulin resistance. In Chlorogenic acid-treated rats, fasting plasma cholesterol and triacylglycerols concentrations significantly decreased by 44% and 58% respectively, as did in liver triacylglycerols concentrations (24%). We did not find differences (p > 0.05) in adipose triacylglycerols concentration. Significant differences (p < 0.05) in the plasma, liver, and spleen concentration of selected minerals were found in Chlorogenic acid-treated rats. In vivo, Chlorogenic acid was found to improve glucose tolerance, decreased some plasma and liver lipids, and improve mineral pool distribution under the conditions of this study.|
Antiviral Res. 2009 Aug;83(2):186-90.
|Anti-hepatitis B virus activity of chlorogenic acid, quinic acid and caffeic acid in vivo and in vitro.[Pubmed: 19463857 ]|
|Chlorogenic acid and its related compounds are abundant plant polyphenols that have a diverse antiviral activity. In this study, HepG2.2.15 cells and duck hepatitis B virus infection model were used as in vitro and in vivo models to evaluate their anti-HBV activity. In the cell model, all the three compounds inhibited HBV-DNA replication as well as HBsAg production. Chlorogenic acid and caffeic acid also reduced serum DHBV level in DHBV-infected duckling model. Moreover, the anti-HBV activity of crude extracts of coffee beans, which have a high content of Chlorogenic acid, was studied. Both the extracts of regular coffee and that of decaffeinated coffee showed inhibitory effect on HBV replication.|