• ChemFaces is a professional high-purity natural products manufacturer.
  • Product Intended Use
  • 1. Reference standards
  • 2. Pharmacological research
  • 3. Inhibitors
  • Home
  • Natural Products
  • Bioactive
  • Screening Libraries
  • Hot Products
  • Plant Catalog
  • Customer Support
  • Product Use Citation
  • About Us
  • Contact Us
  • Natural Products
    Columbianadin
    Information
    CAS No. 5058-13-9 Price $98 / 20mg
    Catalog No.CFN99785Purity>=98%
    Molecular Weight328.36Type of CompoundCoumarins
    FormulaC19H20O5Physical DescriptionWhite cryst.
    Download Manual    COA    MSDSSimilar structuralComparison (Web)
    How to Order
    Orders via your E-mail:

    1. Product number / Name / CAS No.
    2. Delivery address
    3. Ordering/billing address
    4. Contact information
    Sent to Email: info@chemfaces.com
    Contact Us
    Order & Inquiry & Tech Support

    Tel: (0086)-27-84237683
    Fax: (0086)-27-84254680
    E-mail: manager@chemfaces.com
    Address: No. 83, CheCheng Rd., WETDZ, Wuhan, Hubei 430056, PRC
    Delivery time
    Delivery & Payment method

    1. Usually delivery time: Next day delivery by 9:00 a.m. Order now

    2. We accept: Wire transfer & Credit card & Paypal & Western Union
    * Packaging according to customer requirements(5mg, 10mg, 20mg and more). We shipped via FedEx, DHL, UPS, EMS and others courier.
    Our products had been exported to the following research institutions and universities, And still growing.
  • Uniwersytet Medyczny w ?odzi (Poland)
  • Max Rubner-Institut (MRI) (Germany)
  • John Innes Centre (United Kingdom)
  • Sapienza University of Rome (Italy)
  • University of Oslo (Norway)
  • The Vancouver Prostate Centre (V... (Canada)
  • Deutsches Krebsforschungszentrum (Germany)
  • Auburn University (USA)
  • University of Malaya (Malaysia)
  • Instytut Nawozów Sztucznych w P... (Poland)
  • University of Amsterdam (Netherlands)
  • More...
  • Package
    Featured Products
    alpha-Amyrin

    Catalog No: CFN92377
    CAS No: 638-95-9
    Price: $318/5mg
    (-)-Pinoresinol

    Catalog No: CFN92287
    CAS No: 81446-29-9
    Price: $268/5mg
    Ginsenoside Rg6

    Catalog No: CFN90565
    CAS No: 147419-93-0
    Price: $488/10mg
    Isochlorogenic acid B

    Catalog No: CFN99119
    CAS No: 14534-61-3
    Price: $108/20mg
    Ginsenoside Rh3

    Catalog No: CFN99972
    CAS No: 105558-26-7
    Price: $218/20mg
    Biological Activity
    Description: Columbianadin has analgesic, anti-inflammatory, calcium-channel blocking, and platelet aggregation inhibiting functions. It can effectively suppress the growth of colon cancer cells, the induction of apoptosis was correlated with the modulation of caspase-9, caspase-3, Bax, Bcl-2, RIP-3, and caspase-8, Bim and Bid.
    Targets: Caspase | Bcl-2/Bax | ROS | IL Receptor | NO | Calcium Channel | RIP-3 | SOD | GPx-1
    In vitro:
    Phytochemistry. 2012 Sep;81:109-16.
    Biotransformation of columbianadin by rat hepatic microsomes and inhibition of biotransformation products on NO production in RAW 264.7 cells in vitro.[Pubmed: 22784551]
    Columbianadin (CBN, 1), 1-[(8S)-8,9-dihydro-2-oxo-2H-furo[2,3-h]-1-benzopyran-8-yl]-1-methylethyl-[(2Z)-2-methyl-2-butenoic acid]ester is a coumarin-type compound and one of the main bioactive constituents of the underground part of Angelica pubescens Maxim. f. biserrata Shan et Yuan. Although numerous investigations have been undertaken to study the biological activities of Columbianadin, such as analgesic, anti-inflammatory, calcium-channel blocking, and platelet aggregation inhibiting functions, little attention has been paid to its metabolism and/or biotransformation.
    METHODS AND RESULTS:
    Biotransformation of Columbianadin by rat liver microsomes in vitro was studied, and thirteen biotransformation products including eight hitherto unknown compounds [columbianadiratimetins A-H (3-10)] and five known compounds [Columbianadin oxide (2), (+)-2,3-dihydro-4-hydroxy-2-(1-hydroxy-1-methylethyl)-5-benzofurancarboxaldehyde (11), oroselol (12), columbianetin (13), and vaginol (14)] were produced by liver microsomes from rats pre-treated with sodium phenobarbital. The structures of these compounds were elucidated on the basis of extensive spectroscopic analyses which included IR, UV, EIMS, HRESIMS, 1D NMR and 2D NMR, respectively.
    CONCLUSIONS:
    The inhibition of Columbianadin and its main biotransformation products on nitric oxide production induced by lipopolysaccharide was assayed in RAW 264.7 cells at concentrations ranging from 10 to 200 μM to evaluate the biological significance of biotransformation.
    In vivo:
    J Ethnopharmacol. 2014 Sep 11;155(2):1353-61.
    Inhibition of airway inflammation by the roots of Angelica decursiva and its constituent, columbianadin.[Pubmed: 25068578]
    The roots of Angelica decursiva Fr. Et Sav (Umbelliferae) have been frequently used in traditional medicine as anti-inflammatory, antitussive, analgesic agents and expectorant, especially for treating cough, asthma, bronchitis and upper respiratory tract infections. To establish the scientific rationale for the clinical use of Angelica decursiva and to identify new agents for treating inflammatory lung disorders, pharmacological evaluation of the roots of Angelica decursiva and the isolated constituents was performed.
    METHODS AND RESULTS:
    In vitro study was carried out using two lung cells, lung epithelial cells (A549) and alveolar macrophages (MH-S). The inflammatory markers such as IL-6 and nitric oxide (NO) for each cell line were examined. For in vivo study, a mouse model of lipopolysaccharide (LPS)-induced acute lung injury was used and the effects on lung inflammation were established by measuring the cell numbers in bronchoalveolar lavage fluid (BALF) and by histological observation. Water and 70% ethanol extracts of the roots of Angelica decursiva showed considerable inhibitory activity against LPS-induced lung inflammation in mice following oral administration at a dose of 400 mg/kg. Five coumarin derivatives including Columbianadin, umbelliferone, umbelliferone 6-carboxylic acid, nodakenin and nodakenetin were isolated. Among the isolated compounds, Columbianadin was found to possess strong inhibitory activity against the inflammatory response of IL-1β-treated A549 cells and LPS-treated MH-S cells. Columbianadin was found to inhibit NO production by down-regulation of inducible NO synthase. Moreover, Columbianadin was also proved to possess significant inhibitory activity against LPS-induced lung inflammation following oral administration at a dose of 20-60 mg/kg.
    CONCLUSIONS:
    The roots of Angelica decursiva were proved to be effective in the treatment of lung inflammation. Columbianadin can be a potential new agent for treating inflammatory lung disorders.
    Columbianadin Description
    Source: The herbs of Angelicae pubescens
    Solvent: Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.
    Storage: Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Need more advice on solubility, usage and handling? Please email to: service@chemfaces.com

    After receiving: The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
    Recent ChemFaces New Products and Compounds
    Isoliquiritin apioside

    Catalog No: CFN90800
    CAS No: 120926-46-7
    Price: $288/10mg
    Osthenol

    Catalog No: CFN91058
    CAS No: 484-14-0
    Price: $298/10mg
    Epivogeloside

    Catalog No: CFN99283
    CAS No: 118627-52-4
    Price: $368/5mg
    Juglanin

    Catalog No: CFN96238
    CAS No: 5041-67-8
    Price: $368/5mg
    Methyl lucidente G

    Catalog No: CFN95058
    CAS No: 102607-20-5
    Price: $413/5mg
    Matairesinoside

    Catalog No: CFN89301
    CAS No: 23202-85-9
    Price: $288/5mg
    Shancigusin I

    Catalog No: CFN95061
    CAS No: 1435488-35-9
    Price: $388/5mg
    Poricoic acid A(F)

    Catalog No: CFN92838
    CAS No: 137551-38-3
    Price: $218/10mg
    Recently, ChemFaces products have been cited in many studies from excellent and top scientific journals

    Cell. 2018 Jan 11;172(1-2):249-261.e12.
    doi: 10.1016/j.cell.2017.12.019.

    PMID: 29328914

    Mol Cell. 2017 Nov 16;68(4):673-685.e6.
    doi: 10.1016/j.molcel.2017.10.022.

    PMID: 29149595

    Scientific Reports 2017 Dec 11;7(1):17332.
    doi: 10.1038/s41598-017-17427-6.

    PMID: 29230013

    Molecules. 2017 Oct 27;22(11). pii: E1829.
    doi: 10.3390/molecules22111829.

    PMID: 29077044

    J Cell Biochem. 2018 Feb;119(2):2231-2239.
    doi: 10.1002/jcb.26385.

    PMID: 28857247

    Phytomedicine. 2018 Feb 1;40:37-47.
    doi: 10.1016/j.phymed.2017.12.030.

    PMID: 29496173
    Calculate Dilution Ratios(Only for Reference)
    1 mg 5 mg 10 mg 20 mg 25 mg
    1 mM 3.0454 mL 15.2272 mL 30.4544 mL 60.9088 mL 76.1359 mL
    5 mM 0.6091 mL 3.0454 mL 6.0909 mL 12.1818 mL 15.2272 mL
    10 mM 0.3045 mL 1.5227 mL 3.0454 mL 6.0909 mL 7.6136 mL
    50 mM 0.0609 mL 0.3045 mL 0.6091 mL 1.2182 mL 1.5227 mL
    100 mM 0.0305 mL 0.1523 mL 0.3045 mL 0.6091 mL 0.7614 mL
    * Note: If you are in the process of experiment, it's need to make the dilution ratios of the samples. The dilution data of the sheet for your reference. Normally, it's can get a better solubility within lower of Concentrations.
    Protocol
    Kinase Assay:
    Planta Med. 1990 Feb;56(1):127-9.
    The furanocoumarin columbianadin inhibits depolarization induced Ca2+ uptake in rat pituitary GH3 cells.[Pubmed: 2162545 ]
    The furanocoumarin Columbianadin inhibits depolarization induced Ca2+ uptake in rat pituitary GH3 cells.
    Cell Research:
    Biomol Ther (Seoul). 2016 May 1;24(3):320-7.
    Columbianadin Inhibits Cell Proliferation by Inducing Apoptosis and Necroptosis in HCT116 Colon Cancer Cells.[Pubmed: 27098859 ]
    Columbianadin (CBN), a natural coumarin from Angelica decursiva (Umbelliferae), is known to have various biological activities including anti-inflammatory and anti-cancer effects.
    METHODS AND RESULTS:
    In this study, the anti-proliferative mechanism of actions mediated by CBN was investigated in HCT-116 human colon cancer cells. CBN effectively suppressed the growth of colon cancer cells. Low concentration (up to 25 μM) of CBN induced apoptosis, and high concentration (50 μM) of CBN induced necroptosis. The induction of apoptosis by CBN was correlated with the modulation of caspase-9, caspase-3, Bax, Bcl-2, Bim and Bid, and the induction of necroptosis was related with RIP-3, and caspase-8. In addition, CBN induced the accumulation of ROS and imbalance in the intracellular antioxidant enzymes such as SOD-1, SOD-2, catalase and GPx-1.
    CONCLUSIONS:
    These findings demonstrate that CBN has the potential to be a candidate in the development of anti-cancer agent derived from natural products.
    Animal Research:
    Planta Med., 2014,.80 - P2O53.
    Protective effects of the roots of Angelica decursiva and its active compound, columbianadin in lung inflammation[Reference: WebLink]

    METHODS AND RESULTS:
    The water and 70% ethanol extracts of the roots of A. decursiva showed considerable inhibitory activity against LPS-induced lung inflammation in mice following oral administration at a dose of 400 mg/kg. Five coumarin derivatives including Columbianadin, umbelliferone, umbelliferone 6-carboxylic acid, nodakenin and nodakenetin were isolated. Among the isolated compounds, Columbianadin was found to possess strong inhibitory activity against the inflammatory response of IL-1β-treated A549 cells and LPS-treated MH-S cells. Columbianadin was found to inhibit NO production by down-regulation of inducible NO synthase [3]. Moreover, Columbianadin was also proved to possess significant inhibitory activity against LPS-induced lung inflammation following oral administration at a dose of 20 – 60 mg/kg.
    CONCLUSIONS:
    The roots of A. decursiva was proved to be effective in the treatment of lung inflammation. Columbianadin can be a potential new agent for treating inflammatory lung disorders.