|Source:||The seeds of Astragalus complanatus|
|Biological Activity or Inhibitors:|| 1. Complanatoside A is a flavonol glycoside isolated from Astragalus complanatus, and currently it is used as a quality control index for A. complanatus in the 2010 edition of the Chinese Pharmacopoeia.
|Solvent:||Pyridine, Methanol, Ethanol, etc.|
|Storage:||Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please email to: firstname.lastname@example.org
|After receiving:||The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.|
|1 mg||5 mg||10 mg||20 mg||25 mg|
|1 mM||1.5564 mL||7.7821 mL||15.5642 mL||31.1284 mL||38.9105 mL|
|5 mM||0.3113 mL||1.5564 mL||3.1128 mL||6.2257 mL||7.7821 mL|
|10 mM||0.1556 mL||0.7782 mL||1.5564 mL||3.1128 mL||3.8911 mL|
|50 mM||0.0311 mL||0.1556 mL||0.3113 mL||0.6226 mL||0.7782 mL|
|100 mM||0.0156 mL||0.0778 mL||0.1556 mL||0.3113 mL||0.3891 mL|
Zhongguo Zhong Yao Za Zhi. 2005 Apr;30(8):600-2.
|[Determination of complanatoside A in semen Astragali complanati by HPLC].[Pubmed: 16011285]|
|OBJECTIVE: To establish the determination method for Complanatoside A in seeds of Astragalus complanatus. METHOD: An HPLC method has been developed to separate Complanatoside A on ZORBAX EXTEND-C18 (4.6 mm x 250 mm, 5 microm) column with acetonitrile-water-phosphoric acid (20:80:0.2) as mobile phase and UV detection at 267 nm. RESULT: The good linearity of Complanatoside A ranged 0.086-0.430 microg, r = 0.9999. An average recovery of 99.8% (n = 5) was obtained with a RSD of 1.0%. CONCLUSION: The established method is proved to be stability, fast, accurate and can be used for quantification of Complanatoside A in Semen Astragali Complanati.|
Chinese Journal of Pharmaceutical Analysis, Volume 27, Number 5, May 2007, pp. 742-745(4)
|Compare with the content of complanatoside A of commercial Semen Astragali Complanati in different districts[Reference: WebLink]|
|Use the method of HPLC to determine the content of Complanatoside A in commercial Semen Astragali Complanati in different districts.Method:An HPLC method has been developed to determine Complanatoside A in Semen Astragali Complanati from different districts.The mobile phase was acetonitrile-water-phosphoric acid (20:80:0.2)at a flow rate of 1.0 mL·min-1;the detected wavelength was 267 nm.Result:The Complanatoside A content of Semen Astragali Complanati was markedly different compared with different districts.Conclusion:The Semen Astragali Complanati in Shaanxi has the highest content of Complanatoside A and the method is stability,fast and accurate.|
Biomed Chromatogr. 2016 Jun;30(6):888-93.
|Quantification of complanatoside A in rat plasma using LC-MS/MS and its application to a pharmacokinetic study.[Pubmed: 26393341]|
|Complanatoside A is a flavonol glycoside isolated from Astragalus complanatus, and currently it is used as a quality control index for A. complanatus in the 2010 edition of the Chinese Pharmacopoeia. For the first time, a simple and sensitive LC-MS/MS method was developed for the determination of Complanatoside A in rat plasma over the range of 2.3-575 ng/mL. Complanatoside A was extracted from plasma by a protein precipitation procedure, separated by LC and detected by MS/MS in positive electrospray ionization mode. The method was validated for selectivity, carryover, sensitivity, linearity, extraction recovery, matrix effect, accuracy, precision and stability studies. The lower limit of quantification was established at 2.3 ng/mL. Intra- and inter-day precisions (LLOQ, low-QC, med-QC and high-QC) were <7.9%, and accuracies were between 94.0 and 105.1%. Matrix effect was acceptable (97.9-103.0%) and extraction recovery was reproducible (88.5-94.4%). Complanatoside A was stable in the investigated conditions. The method was applied to the pharmacokinetics of Complanatoside A in rats.|