|Source:||The herbs of Chrysanthemum indicum|
|Biological Activity or Inhibitors:||1. Cyanidin-3- O -glucoside has protective effects from blackberry extract against peroxynitrite-induced endothelial dysfunction and vascular failure.
2. Cyanidin-3- O -glucoside exhibits strong antioxidant activities including DPPH radical scavenging activities, ABTS radical scavenging activities and ORAC values.
3. Cyanidin-3-O-glucoside chloride decreases mutant p21 expression, and increases the ratio of Bax/Bcl-2 and the activation of caspase-3 to induce apoptosis.
|Solvent:||Pyridine, Methanol, Ethanol, etc.|
|Storage:||Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please email to: email@example.com
|After receiving:||The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.|
|1 mg||5 mg||10 mg||20 mg||25 mg|
|1 mM||2.2253 mL||11.1264 mL||22.2529 mL||44.5058 mL||55.6322 mL|
|5 mM||0.4451 mL||2.2253 mL||4.4506 mL||8.9012 mL||11.1264 mL|
|10 mM||0.2225 mL||1.1126 mL||2.2253 mL||4.4506 mL||5.5632 mL|
|50 mM||0.0445 mL||0.2225 mL||0.4451 mL||0.8901 mL||1.1126 mL|
|100 mM||0.0223 mL||0.1113 mL||0.2225 mL||0.4451 mL||0.5563 mL|
Life Sci. 2003 Jul 18;73(9):1097-114.
|Protective effects of cyanidin-3-O-glucoside from blackberry extract against peroxynitrite-induced endothelial dysfunction and vascular failure.[Pubmed: 12818719]|
|Vascular rings exposed to peroxynitrite exhibited reduced endothelium-dependent relaxant responses in response to acetylcholine as well as a vascular contractility dysfunction in response to norepinephrine. The development of this peroxynitrite-induced vascular dysfunction was ameliorated by the blackberry juice (different dilutions that contained 80 ppm;40 ppm;14.5 ppm of cyanidin-3-O-glucoside) and cyanidin-3-O-glucoside (as chloride,Cyanidin-3-O-glucoside chloride ) (0.085 microM;0.028 microM;0.0085 microM). In conclusion our findings clearly demonstrate that blackberry juice containing cyanidin-3-O-glucoside is a scavenger of peroxynitrite and that exert a protective effect against endothelial dysfunction and vascular failure induced by peroxynitrite.|
BMC Complement Altern Med. 2015; 15: 313.
|Antioxidant and in vitro anticancer activities of phenolics isolated from sugar beet molasses[Pubmed: 26347338 ]|
|Background In the present study, the phenolic compounds were prepared using ultrasonic-aid extraction from sugar beet molasses (SBM). Methods Gallic acid (GA), Cyanidin-3-O-glucoside chloride (CGC) and epicatechin (EP) were produced after column chromatography from the extraction, and further detected using NMR, QTOF-MS and ESI-MS/MS. Results The three compounds exhibited strong antioxidant activities including DPPH radical scavenging activities, ABTS radical scavenging activities and ORAC values. GA showed the strongest antioxidant activity. Antitumor activities significantly increased in a dose-dependent manner. In particular, the CGC had growth inhibitory activities of 94.86, 87.27 and 67.13 % against the human colon (CACO-2), hepatocellular (HepG2) and breast (MCF-7) carcinoma cell lines, respectively, at the highest concentration of 400 μg/mL of the extracts. These results suggest that the three compounds are key chemical compositions valuable for preparing functional foods in the food industry. Conclusions The results suggested that SBM is a natural source of antioxidant and antitumor agents for preparing functional foods.|
Int J Mol Sci. 2016 Jul; 17(7): 993.
|Cytotoxicity and Apoptotic Effects of Polyphenols from Sugar Beet Molasses on Colon Carcinoma Cells in Vitro[Pubmed: 27347927]|
|Three polyphenols were isolated and purified from sugar beet molasses by ultrasonic-aid extraction and various chromatographic techniques, and their structures were elucidated by spectral analysis. Cytotoxicity and the molecular mechanism were measured by methyl thiazolyl tetrazolium (MTT) assay, flow cytometry, caspase-3 activity assay and Western blot assay. The results showed that gallic acid, Cyanidin-3-O-glucoside chloride and epicatechin have cytotoxicity to the human colon, hepatocellular and breast cancer cells. Cyanidin-3-O-glucoside chloride showed its cytotoxicity against various tumor cell lines, particularly against colon cancer Caco-2 cells with half maximal inhibitory concentration (IC50) value of 23.21 ± 0.14 μg/mL in vitro. Cyanidin-3-O-glucoside chloride may be a potential candidate for the treatment of colon cancer. In the mechanism study, Cyanidin-3-O-glucoside chloride increased the ratio of cell cycle at G0/G1 phase and reduced cyclin D1 expression on Caco-2 cells. Cyanidin-3-O-glucoside chloride decreased mutant p21 expression, and increased the ratio of Bax/Bcl-2 and the activation of caspase-3 to induce apoptosis.|