ChemFaces is a professional high-purity natural products manufacturer.
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More articles cited ChemFaces products.
The Japan Society for Analy. Chem.2017 Nov. 8;Plant Cell, (PCTOC)05 November 2016Mol Cells.2015 Sep 30;38(9):765-72.Virulence.2018 Jan 1;BMC Complement Altern Med.2017 Aug 9;
Front Immunol.2017 Nov 13;Phytomedicine2015 August 14Invest New Drugs. 2017 Apr;Inflammation.2015 Feb;38(1):445-55. Int J Mol Sci.2017 Nov 30;
Korean J. of Food Sci. and TechApril 2016Evid Based Complement Alternat Med. 2016 Jun 13.Int J Mol Sci. 2017 Dec 21;Food and Bioprocess TechnologyJune 2017
Our products had been exported to the following research institutions and universities, And still growing.
University of Liège (Belgium)Sapienza University of Rome (Italy)Seoul National University (Korea)Complutense University of Madrid (Spain)
Donald Danforth Plant Science Ce... (USA)Almansora University (Egypt)Charles Sturt University (Denmark)University of Pretoria (South Africa)
Institute of Chinese Materia Med... (China)University of Helsinki (Finland)Universiti Kebangsaan Malaysia (Malaysia)
||Dihydrochelerythrine has antifungal activity against pathogenic plant fungi; it shows antiparasitic efficacy against Ichthyophthirius multifiliis in richadsin, it has potential application in the therapy of serious infection caused by I. multifiliis. Dihydrochelerythrine affects cell cycle distribution, activates mitochondrial apoptotic pathway, and induces apoptosis and necrosis in HL-60 cells.|
||Antifection | Caspase|
|Nat Prod Res. 2011 Jul;25(11):1082-9. |
|Inhibitory activity of dihydrosanguinarine and dihydrochelerythrine against phytopathogenic fungi.[Pubmed: 21500094]|
|The antifungal activities of dihydrosanguinarine and Dihydrochelerythrine, isolated from the leaves of Macleaya microcarpa, were evaluated on 12 plant pathogenic fungi; the two compounds exhibited the highest antifungal activity against Botrytis cinerea Pers.
METHODS AND RESULTS:
Among the 11 tested plant pathogenic fungi in vitro, the two compounds showed the highest antifungal activity against B. cinerea Pers, with 95.16% and 98.32% mycelial growth inhibition at 50 μg mL⁻1, respectively. In addition, the two compounds inhibited spore germination in vitro in a concentration-dependent manner. They also showed potent protective and curative effects against Erysiphe graminis and B. cinerea in vivo.
This is the first report on the antifungal activity of dihydrosanguinarine and Dihydrochelerythrine against pathogenic plant fungi.
|Vet Parasitol. 2011 Dec 29;183(1-2):8-13. |
|Antiparasitic efficacy of dihydrosanguinarine and dihydrochelerythrine from Macleaya microcarpa against Ichthyophthirius multifiliis in richadsin (Squaliobarbus curriculus).[Pubmed: 21813242]|
|Ichthyophthirius multifiliis is a holotrichous protozoan that invades the gills and skin surfaces of fish and can cause morbidity and high mortality in most species of freshwater fish worldwide.
The present study was undertaken to investigate the antiparasitic activity of crude extracts and pure compounds from the leaves of Macleaya microcarpa.
METHODS AND RESULTS:
The chloroform extract showed a promising antiparasitic activity against I. multifiliis. Based on these finding, the chloroform extract was fractionated on silica gel column chromatography in a bioactivity-guided isolation affording two compounds showing potent activity. The structures of the two compounds were elucidated as dihydrosanguinarine and Dihydrochelerythrine by hydrogen and carbon-13 nuclear magnetic resonance spectrum and electron ionization mass spectrometry. The in vivo tests revealed that dihydrosanguinarine and Dihydrochelerythrine were effective against I. multifiliis with median effective concentration (EC(50)) values of 5.18 and 9.43 mg/l, respectively. The acute toxicities (LC(50)) of dihydrosanguinarine and Dihydrochelerythrine for richadsin were 13.3 and 18.2mg/l, respectively.
The overall results provided important information for the potential application of dihydrosanguinarine and Dihydrochelerythrine in the therapy of serious infection caused by I. multifiliis.
||The herbs of Chelidonium majus
||Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.
||Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please email to: firstname.lastname@example.org
||The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
Recent ChemFaces New Products and Compounds
Recently, ChemFaces products have been cited in many studies from excellent and top scientific journals
Cell. 2018 Jan 11;172(1-2):249-261.e12. doi: 10.1016/j.cell.2017.12.019.PMID: 29328914
Mol Cell. 2017 Nov 16;68(4):673-685.e6. doi: 10.1016/j.molcel.2017.10.022.PMID: 29149595
Scientific Reports 2017 Dec 11;7(1):17332.doi: 10.1038/s41598-017-17427-6.PMID: 29230013
Molecules. 2017 Oct 27;22(11). pii: E1829.doi: 10.3390/molecules22111829.PMID: 29077044
J Cell Biochem. 2018 Feb;119(2):2231-2239.doi: 10.1002/jcb.26385. PMID: 28857247
Phytomedicine. 2018 Feb 1;40:37-47. doi: 10.1016/j.phymed.2017.12.030.PMID: 29496173
Calculate Dilution Ratios(Only for Reference)
* Note: If you are in the process of experiment, it's need to make the dilution ratios of the samples. The dilution data of the sheet for your reference. Normally, it's can get a better solubility within lower of Concentrations.
|Toxicol In Vitro. 2008 Jun;22(4):1008-17. |
|Chelerythrine and dihydrochelerythrine induce G1 phase arrest and bimodal cell death in human leukemia HL-60 cells.[Pubmed: 18358694]|
|A quaternary benzo[c]phenanthridine alkaloid chelerythrine displays a wide range of biological activities including cytotoxicity to normal and cancer cells. In contrast, less is known about the biological activity of Dihydrochelerythrine, a product of chelerythrine reduction.
METHODS AND RESULTS:
We examined the cytotoxicity of chelerythrine and Dihydrochelerythrine in human promyelocytic leukemia HL-60 cells. After 4h of treatment, chelerythrine induced a dose-dependent decrease in the cell viability with IC50 of 2.6 microM as shown by MTT reduction assay. Dihydrochelerythrine appeared to be less cytotoxic since the viability of cells exposed to 20 microM Dihydrochelerythrine for 24h was reduced only to 53%. Decrease in the viability induced by both alkaloids was accompanied by apoptotic events including the dissipation of mitochondrial membrane potential, activation of caspase-9 and -3, and appearance of cells with sub-G1 DNA. Moreover, chelerythrine, but not Dihydrochelerythrine, elevated the activity of caspase-8. A dose-dependent induction of apoptosis and necrosis by chelerythrine and Dihydrochelerythrine was confirmed by annexin V/propidium iodide dual staining flow cytometry. Besides, both alkaloids were found to induce accumulation of HL-60 cells in G1 phase of the cell cycle.
We conclude that both chelerythrine and Dihydrochelerythrine affect cell cycle distribution, activate mitochondrial apoptotic pathway, and induce apoptosis and necrosis in HL-60 cells.
|J Chromatogr B Analyt Technol Biomed Life Sci. 2013 Dec 15;941:17-24. |
|Mass spectrometric investigation of chelerythrine and dihydrochelerythrine biotransformation patterns in human hepatocytes.[Pubmed: 24184831]|
|The quaternary benzo[c]phenanthridine alkaloids (QBAs) are an important subgroup of plant secondary metabolites. Their main representatives, sanguinarine (SG) and chelerythrine (CHE), have pleiotropic biological effects and a wide spectrum of medicinal applications. The biotransformation of SG and CHE has only been partially studied while subsequent oxidative transformation of their dihydro derivates, the main metabolites, is practically unknown. The aim of this study was to characterize the biotransformation of CHE and Dihydrochelerythrine (DHCHE) in detail, with respect to their more extensive biotransformation than SG.
METHODS AND RESULTS:
Phase I as well as phase II biotransformation of both compounds was examined in human hepatocyte suspensions. Liquid chromatography with electrospray-quadrupole time-of-flight mass spectrometry (LC-ESI-QqTOF MS) was used for analysis of the metabolites. Using the LC-ESI-QqTOF MS method, we analyzed and then suggested the putative structures of 11 phase I and 5 phase II metabolites of CHE, and 11 phase I and 6 phase II metabolites of DHCHE. For the most abundant metabolites of CHE, DHCHE and O-demethylated DHCHE, their cytotoxicity on primary cultures of human hepatocytes was analyzed.
Both metabolites were nontoxic up to 50μM concentration and this indicates decreasing toxic effects for CHE biotransformation products, i.e. DHCHE and O-demethylated DHCHE.