|Source:||The fruit body of Ganoderma lucidum|
|Biological Activity or Inhibitors:||1. Ganoderiol F has anti-inflammatory activity.
2. Ganoderiol F shows cytotoxic and anti-HIV activities.
3. Ganoderiol F induces growth arrest of cancer cell lines HepG2, Huh7 and K562.
4. Ganoderiol F inhibits activity of topoisomerases in vitro.
5. Ganoderiol F inhibits human immunodeficiency virus-1 protease with IC(50) values of 20-40 microM.
|Solvent:||Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.|
|Storage:||Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please email to: email@example.com
|After receiving:||The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.|
|1 mg||5 mg||10 mg||20 mg||25 mg|
|1 mM||2.1993 mL||10.9963 mL||21.9925 mL||43.985 mL||54.9813 mL|
|5 mM||0.4399 mL||2.1993 mL||4.3985 mL||8.797 mL||10.9963 mL|
|10 mM||0.2199 mL||1.0996 mL||2.1993 mL||4.3985 mL||5.4981 mL|
|50 mM||0.044 mL||0.2199 mL||0.4399 mL||0.8797 mL||1.0996 mL|
|100 mM||0.022 mL||0.11 mL||0.2199 mL||0.4399 mL||0.5498 mL|
Toxicol Appl Pharmacol. 2014 Nov 1;280(3):434-42.
|Anti-inflammatory and heme oxygenase-1 inducing activities of lanostane triterpenes isolated from mushroom Ganoderma lucidum in RAW264.7 cells.[Pubmed: 25239868]|
|Ganoderma lucidum is a popular medicinal mushroom used in traditional medicine for preventing or treating a variety of diseases. In the present study, we investigated the anti-inflammatory and heme oxygenase (HO)-1 inducing effects of 12 lanostane triterpenes from G. lucidum in RAW264.7 cells. Of these, seven triterpenes, butyl lucidenateE2, butyl lucidenateD2 (GT-2), butyl lucidenate P, butyl lucidenateQ, Ganoderiol F, methyl ganodenate J and butyl lucidenate N induced HO-1 expression and suppressed lipopolysaccharide (LPS)-induced nitric oxide (NO) production. Inhibiting HO-1 activity abrogated the inhibitory effects of these triterpenes on the production of NO in LPS-stimulated RAW264.7 cells, suggesting the involvement of HO-1 in the anti-inflammatory effects of these triterpenes.|
Chem Pharm Bull (Tokyo). 2009 Oct;57(10):1076-80.
|Anti-human immunodeficiency virus-1 protease activity of new lanostane-type triterpenoids from Ganoderma sinense.[Pubmed: 19801861]|
|Among these, ganoderic acid GS-2, 20-hydroxylucidenic acid N, 20(21)-dehydrolucidenic acid N and Ganoderiol F inhibited human immunodeficiency virus-1 protease with IC(50) values of 20-40 microM.|
Life Sci. 2006 Aug 15;79(12):1129-39.
|Ganoderiol F, a ganoderma triterpene, induces senescence in hepatoma HepG2 cells.[Pubmed: 16635496]|
|Ganoderiol F (GolF), a tetracyclic triterpene, was isolated from Ganoderma amboinense and found to induce senescence of cancer cell lines. GolF induced growth arrest of cancer cell lines HepG2, Huh7 and K562, but exerted much less effect in hepatoma Hep3B cells and normal lung fibroblast MRC5 cells, and no effect on peripheral blood mononuclear cells. GolF was found to inhibit activity of topoisomerases in vitro, which may contribute to the inhibition of cellular DNA synthesis. Activation of the mitogen-activated protein kinase EKR and up-regulation of cyclin-dependent kinase inhibitor p16 were found in early stages of GolF treatment and were presumed to cause cell-cycle arrest and trigger premature senescence of HepG2 cells. The growth-arrest and senescence induction capability on cancer cells suggest anticancer potential of GolF.|