|Source:||The herbs of Alnus japonica|
|Biological Activity or Inhibitors:|| 1. (+)-Hannokinol and MESO-hannokinol can significantly inhibit lipopolysaccharide-induced nitric oxide production in BV2 microglial cells at concentrations ranging from 1 microM to 100 microM.
|Solvent:||Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.|
|Storage:||Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please email to: email@example.com
|After receiving:||The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.|
|1 mg||5 mg||10 mg||20 mg||25 mg|
|1 mM||3.1606 mL||15.8028 mL||31.6056 mL||63.2111 mL||79.0139 mL|
|5 mM||0.6321 mL||3.1606 mL||6.3211 mL||12.6422 mL||15.8028 mL|
|10 mM||0.3161 mL||1.5803 mL||3.1606 mL||6.3211 mL||7.9014 mL|
|50 mM||0.0632 mL||0.3161 mL||0.6321 mL||1.2642 mL||1.5803 mL|
|100 mM||0.0316 mL||0.158 mL||0.3161 mL||0.6321 mL||0.7901 mL|
Planta Med. 2008 Jun;74(8):867-9.
|Inhibitory constituents of lipopolysaccharide-induced nitric oxide production in BV2 microglia isolated from Amomum tsao-ko.[Pubmed: 18523923 ]|
|A methanolic extract of the fruits of AMOMUM TSAO-KO (Zingiberaceae) significantly attenuated nitric oxide production in lipopolysaccharide-simulated BV2 microglia. Two new bicyclic nonanes characterized as 6,7-dihydroxy-indan-4-carbaldehyde ( 1) and 6-hydroxy-indan-4-carbaldehyde ( 2) were isolated with the eleven known compounds 6,7-dihydroxy-3,7-dimethyloct-2-enoic acid ( 3), tsaokoin ( 4), isotsaokoin ( 5), 8-oxogeraniol ( 6), P-menth-1-ene-5,6-diol ( 7), 3alpha-hydroxycarvotagenone ( 8), tsaokoarylone ( 9), 1,7-bis(4-hydroxy-3-methoxyphenyl)-4,6-heptadien-3one ( 10), (+)-Hannokinol ( 11), MESO-Hannokinol ( 12) and hannokinin ( 13), from the fruits of A. TSAO-KO using bioactivity-guided fractionation. All thirteen compounds significantly inhibited lipopolysaccharide-induced nitric oxide production in BV2 microglial cells at concentrations ranging from 1 microM to 100 microM.|
Chinese Journal of Experimental Traditional Medical Formulae, 2015 , 21 (3) :37-40.
|Identification of Chemical Constituents Occurring in Leaves of Alpinia officinarum[Reference: WebLink]|
|The current study aimed to identify the chemical constituents occurring in the leaves of Alpinia officinarum. Method: The collected leaves of A. officinarum were dried in an electric thermostatic drying oven at 40° C overnight. The freshly dried leaves were smashed using a universal high-speed smashing machine and then sieved manually by an 80 mesh. The resulting fine powders and residue were mixed evenly. Twelve flavonoids were chrysin,pinocembrin,tectochrysin,apigenin,galangin,3-methylgalangin,acacetin,kaempferol,kaempferide,quercetin,isorhamnetin and rutin,respectively. Four diarylheptanoids included yakuchinone A,oxyphyllacinol,hexahydrocurcumin and Hannokinol. Conclusion: Sixteen chemicals were identified using LC-MS / MS and some unknown diarylheptanoids need to be identified in the future.|