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    Hematoxylin
    Information
    CAS No. 517-28-2 Price $30 / 20mg
    Catalog No.CFN96490Purity>=98%
    Molecular Weight302.28Type of CompoundFlavonoids
    FormulaC16H14O6Physical DescriptionPowder
    Download     COA    MSDSSimilar structuralComparison (Web)
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    Biological Activity
    Description: Hematoxylin and eosin (H+E) are pigments, they are used to morphological assessment.
    In vivo:
    Stroke. 1993 Jan;24(1):117-21.
    Effect of brain edema on infarct volume in a focal cerebral ischemia model in rats.[Pubmed: 8418534]
    Infarct volume is one of the common indexes for assessing the extent of ischemic brain injury following focal cerebral ischemia. Accuracy in the measurement of infarct volume is compounded by postischemic brain edema that may increase brain volume in the infarcted region. We evaluated the effect of brain edema on infarct volume determined by triphenyltetrazolium chloride and Hematoxylin and eosin stains in a focal cerebral ischemia model in rats.
    METHODS AND RESULTS:
    In a middle cerebral artery occlusion model in rats, infarction is confined to the cerebral cortex. The infarct was delineated by triphenyltetrazolium chloride stain and, in selected samples, by Hematoxylin and eosin stain. We determined infarct size at different times after the ischemic insult (6 hours to 7 days) in relation to the evolution of brain edema by the direct measurement of infarct volume. Indirect measurement to reduce the effect of edema on infarct volume was also conducted in the same brain samples. Direct measurement showed that infarct volume fluctuated with the evolution of brain edema (one-way analysis of variance, p < 0.0001). Infarct volume determined by indirect measurement was independent of the extent of brain edema and remained stable from 6 hours to 3 days after ischemia. There was a good correlation between triphenyltetrazolium chloride and Hematoxylin and eosin stains in delineating infarct volume with both direct and indirect measurement.
    CONCLUSIONS:
    Traditional direct measurement of infarct volume is associated with an overestimation of infarct volume during the development of brain edema in the first 3 days after ischemia. This artifact can be reduced with indirect measurement, which is based on noninfarcted cortex volume.
    Hematoxylin Description
    Source: The heartwoods of Caesalpinia sappan.
    Solvent: Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.
    Storage: Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Need more advice on solubility, usage and handling? Please email to: service@chemfaces.com

    After receiving: The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
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    Recently, ChemFaces products have been cited in many studies from excellent and top scientific journals

    Cell. 2018 Jan 11;172(1-2):249-261.e12.
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    Calculate Dilution Ratios(Only for Reference)
    1 mg 5 mg 10 mg 20 mg 25 mg
    1 mM 3.3082 mL 16.541 mL 33.0819 mL 66.1638 mL 82.7048 mL
    5 mM 0.6616 mL 3.3082 mL 6.6164 mL 13.2328 mL 16.541 mL
    10 mM 0.3308 mL 1.6541 mL 3.3082 mL 6.6164 mL 8.2705 mL
    50 mM 0.0662 mL 0.3308 mL 0.6616 mL 1.3233 mL 1.6541 mL
    100 mM 0.0331 mL 0.1654 mL 0.3308 mL 0.6616 mL 0.827 mL
    * Note: If you are in the process of experiment, it's need to make the dilution ratios of the samples. The dilution data of the sheet for your reference. Normally, it's can get a better solubility within lower of Concentrations.