|Source:||The roots of Lindera aggregata( Sims) Kosterm|
|Biological Activity or Inhibitors:||1. Linderane was characterized as a mechanism-based inactivator of CYP2C9.
|Solvent:||Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.|
|Storage:||Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please email to: firstname.lastname@example.org
|After receiving:||The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.|
|1 mg||5 mg||10 mg||20 mg||25 mg|
|1 mM||3.842 mL||19.2101 mL||38.4202 mL||76.8403 mL||96.0504 mL|
|5 mM||0.7684 mL||3.842 mL||7.684 mL||15.3681 mL||19.2101 mL|
|10 mM||0.3842 mL||1.921 mL||3.842 mL||7.684 mL||9.605 mL|
|50 mM||0.0768 mL||0.3842 mL||0.7684 mL||1.5368 mL||1.921 mL|
|100 mM||0.0384 mL||0.1921 mL||0.3842 mL||0.7684 mL||0.9605 mL|
Zhongguo Zhong Yao Za Zhi. 2004 Jul;29(7):657-9.
|Determination of linderane in root tuber of Lindera aggregata by HPLC[Pubmed: 15503773]|
|OBJECTIVE: To provide scientific basis for quality control of Lindera aggregata. METHOD: HPLC analytical method was established using a Lichrospher C18 column and acetonitrile-water (56:44) as the mobile phase, detected at 235 nm. RESULT: The linear range of Linderane is between 0.0642 - 0.5774 microg, the average recovery was 98.4%, RSD1.7% (n = 9). CONCLUSION: Contents of Linderane in commercially available and collected samples were from 0.028% to 0.123% and from 0.056% to 0.222% respectively.|
Xenobiotica. 2015 Jun 11:1-10.
|Mechanism-based inactivation of CYP2C9 by linderane.[Pubmed: 26068520]|
|1. Linderane (LDR), a furan-containing sesquiterpenoid, is found in Lindera aggregata (Sims) Kosterm, a common traditional Chinese herbal medicine. We thoroughly studied the irreversible inhibitory effect of Linderane on cytochrome P450 2C9 (CYP2C9). 2. Linderane caused a time- and concentration-dependent inactivation of CYP2C9. In addition, the inactivation of CYP2C9 by Linderane was NADPH-dependent and irreversible.Diclofenac, a substrate of CYP2C9, prevented the enzyme from inactivation produced by Linderane. The estimated partition ratio of the inactivation was approximately 227. 3. Two reactive intermediates, including furanoepoxide and γ-ketoenal, might be responsible for the observed enzyme inactivation. The formation of the intermediates was verified by chemical synthesis. Multiple P450 enzymes, including CYPs 1A2, 2B6, 2C9, 2C19, 2D6, 3A4, and 3A5, were found to be involved in the metabolic activation of Linderane. In conclusion, Linderane was characterized as a mechanism-based inactivator of CYP2C9.|