• ChemFaces is a professional high-purity natural products manufacturer.
  • Product Intended Use
  • 1. Reference standards
  • 2. Pharmacological research
  • 3. Inhibitors
  • Home
  • Natural Products
  • Bioactive
  • Screening Libraries
  • Hot Products
  • Plant Catalog
  • Customer Support
  • Product Use Citation
  • About Us
  • Contact Us
  • Natural Products
    Neohesperidin
    Information
    CAS No. 13241-33-3 Price $40 / 20mg
    Catalog No.CFN99125Purity>=98%
    Molecular Weight610.56Type of CompoundFlavonoids
    FormulaC28H34O15Physical DescriptionPowder
    Download Manual    COA    MSDSSimilar structuralComparison (Web)
    How to Order
    Orders via your E-mail:

    1. Product number / Name / CAS No.
    2. Delivery address
    3. Ordering/billing address
    4. Contact information
    Sent to Email: info@chemfaces.com
    Contact Us
    Order & Inquiry & Tech Support

    Tel: (0086)-27-84237683
    Fax: (0086)-27-84254680
    E-mail: manager@chemfaces.com
    Address: No. 83, CheCheng Rd., WETDZ, Wuhan, Hubei 430056, PRC
    Delivery time
    Delivery & Payment method

    1. Usually delivery time: Next day delivery by 9:00 a.m. Order now

    2. We accept: Wire transfer & Credit card & Paypal & Western Union
    * Packaging according to customer requirements(5mg, 10mg, 20mg and more). We shipped via FedEx, DHL, UPS, EMS and others courier.
    Our products had been exported to the following research institutions and universities, And still growing.
  • Auburn University (USA)
  • University of Bonn (Germany)
  • University of Indonesia (Indonesia)
  • Sanford Burnham Medical Research... (USA)
  • Monash University Sunway Campus (Malaysia)
  • Massachusetts General Hospital (USA)
  • Colorado State University (USA)
  • Melbourne University (Australia)
  • University of Zurich (Switzerland)
  • Medical University of South Caro... (USA)
  • University of Limpopo (South Africa)
  • More...
  • Package
    Featured Products
    Afzelin

    Catalog No: CFN98757
    CAS No: 482-39-3
    Price: $288/20mg
    Cryptochlorogenic acid

    Catalog No: CFN99117
    CAS No: 905-99-7
    Price: $128/20mg
    Chlorogenic acid

    Catalog No: CFN99116
    CAS No: 327-97-9
    Price: $30/20mg
    Ganoderic acid C6

    Catalog No: CFN92290
    CAS No: 105742-76-5
    Price: $368/10mg
    Moracin P

    Catalog No: CFN92324
    CAS No: 102841-46-3
    Price: $418/5mg
    Biological Activity
    Description: Neohesperidin, a natural new nutrition sweetener, has antioxidant (IC50=22.31ug/mL), anti-inflammatory, and neuroprotective effects. It can attenuate cerebral ischemia-reperfusion injury via the inhibition of neuronal and oxidative stress through the regulation of the apoptotic pathway and activating the Akt/Nrf2/HO-1 pathway, it may be useful for the treatment and/or protection of gastritis.
    Targets: Beta Amyloid | ROS | Calcium Channel | HO-1 | Nrf2 | Akt | Caspase | Bcl-2/Bax | TNF-α | COX
    In vitro:
    Curr Alzheimer Res. 2015;12(5):424-33.
    Inhibition of β-amyloid Aggregation By Albiflorin, Aloeemodin And Neohesperidin And Their Neuroprotective Effect On Primary Hippocampal Cells Against β-amyloid Induced Toxicity.[Pubmed: 25938872]
    Being one of the hallmarks of Alzheimer's disease, β-amyloid (Aβ) aggregates induce complicated neurotoxicity. Evidences show that the underlying mechanism of neurotoxicity involves a glutamate receptor subtype, N-methyl-D-aspartate (NMDA) receptor, an increase in intracellular calcium(II) ion loading as well as an elevation in oxidation stress.
    METHODS AND RESULTS:
    In this work, among the 35 chemical components of Chinese herbal medicines (CHMs) being screened for inhibitors of Aβ aggregation, four of them, namely albiflorin, aloeemodin, Neohesperidin and physcion, were found for the first time to exhibit a potent inhibitory effect on Aβ(1-40) and Aβ(1-42) aggregation. Their neuroprotective capability on primary hippocampal neuronal cells was also investigated by MTT assay, ROS assay and intracellular calcium(II) ion concentration measurement.
    CONCLUSIONS:
    It was interesting to find that physcion was rather toxic to neuronal cells while albiflorin, aloeemodin and Neohesperidin reduced the toxicity and ROS induced by both monomeric and oligomeric Aβ species. In addition, albiflorin was particularly powerful in maintaining the intracellular Ca(2+) concentration.
    In vivo:
    J Asian Nat Prod Res. 2013 Sep;15(9):1023-37.
    Neohesperidin attenuates cerebral ischemia-reperfusion injury via inhibiting the apoptotic pathway and activating the Akt/Nrf2/HO-1 pathway.[Pubmed: 23952707]
    Oxidative stress is well known to play a pivotal role in cerebral ischemia-reperfusion injury. On the basis of this fact, antioxidative agents have been demonstrated to be neuroprotective. Neohesperidin (NH) is abundant in citrus flavonoids and possesses reactive oxygen species scavenging activity and neuroprotective effects in vitro. However, little is known about its effects on cerebral ischemia-reperfusion injury and the underlying mechanisms.
    METHODS AND RESULTS:
    In this study, we use a rat model of middle cerebral artery occlusion (MCAO) to investigate the neuroprotective effects of NH. NH significantly improved neurological functions and attenuated MCAO-induced infarct volume, pathological changes, and neuronal loss. Moreover, it enhanced antioxidant capacity and suppressed oxidative stress in the brain. NH inhibited the MCAO-induced upregulation of Bax, cytochrome c, and cleaved caspase-9 and -3, as well as the downregulation of Bcl-2. Interestingly, NH treatment upregulated heme oxygenase-1 (HO-1) in a concentration-dependent manner, which was due to the NH-mediated activation of the protein kinase B (Akt)/nuclear factor erythroid 2-related factor 2 (Nrf2) pathway. NH also abolished the MCAO-induced inhibition of the Akt/Nrf2 pathway.
    CONCLUSIONS:
    In conclusion, NH attenuates cerebral ischemia-reperfusion injury via the inhibition of neuronal apoptosis and oxidative stress through the regulation of the apoptotic pathway and the Akt/Nrf2/HO-1 pathway. NH might be a promising preventive agent for ischemic stroke.
    Phytother Res. 2009 Dec;23(12):1748-53
    Protective effects of neohesperidin and poncirin isolated from the fruits of Poncirus trifoliata on potential gastric disease.[Pubmed: 19367677]

    METHODS AND RESULTS:
    The effects of Poncirus trifoliata (P. trifoliata) (Ponciri Fructus, PF) extract and its constituents such as Neohesperidin and poncirin on gastritis in rats and human gastric cancer cells were investigated. The PF 70% ethanol extracts (1 g) showed approximately 11.38% of acid-neutralizing capacities and cytotoxicity (IC50=85.39 microg/mL) against human AGS gastric cancer cells. In addition, Neohesperidin exhibited antioxidant activity (IC50=22.31 microg/mL) in the 1,1-diphenyl-2-picryldydrazyl (DPPH) radical-scavenging assay. Neohesperidin (50 mg/kg) and poncirin (100 mg/kg) significantly inhibited 55.0% and 60.0% of HCl/ethanol-induced gastric lesions, respectively, and increased the mucus content. In pylorus ligated rats, Neohesperidin (50 mg/kg) significantly decreased the volume of gastric secretion and gastric acid output, and increased the pH.
    METHODS AND RESULTS:
    From these results, it could be suggested that Neohesperidin and poncirin isolated from PF may be useful for the treatment and/or protection of gastritis.
    Neohesperidin Description
    Source: The peels of Citrus aurantium L.
    Solvent: DMSO, Pyridine, Methanol, Ethanol, etc.
    Storage: Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Need more advice on solubility, usage and handling? Please email to: service@chemfaces.com

    After receiving: The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
    Recent ChemFaces New Products and Compounds
    Salvianolic acid Y

    Catalog No: CFN91004
    CAS No: 1638738-76-7
    Price: $333/5mg
    (-)-Lyoniresinol 9'-O-glucoside

    Catalog No: CFN96732
    CAS No: 143236-02-6
    Price: $513/5mg
    Tectoruside

    Catalog No: CFN95051
    CAS No: 38784-73-5
    Price: $288/20mg
    Poricoic acid B

    Catalog No: CFN95050
    CAS No: 137551-39-4
    Price: $368/10mg
    Hannokinol

    Catalog No: CFN96248
    CAS No: 79120-40-4
    Price: $368/5mg
    Iristectorin A

    Catalog No: CFN95037
    CAS No: 37744-61-9
    Price: $333/5mg
    Carasinol B

    Catalog No: CFN95042
    CAS No: 777857-86-0
    Price: $333/5mg
    9,10-Dimethoxycanthin-6-one

    Catalog No: CFN92127
    CAS No: 155861-51-1
    Price: $413/5mg
    Recently, ChemFaces products have been cited in many studies from excellent and top scientific journals

    Cell. 2018 Jan 11;172(1-2):249-261.e12.
    doi: 10.1016/j.cell.2017.12.019.

    PMID: 29328914

    Mol Cell. 2017 Nov 16;68(4):673-685.e6.
    doi: 10.1016/j.molcel.2017.10.022.

    PMID: 29149595

    Scientific Reports 2017 Dec 11;7(1):17332.
    doi: 10.1038/s41598-017-17427-6.

    PMID: 29230013

    Molecules. 2017 Oct 27;22(11). pii: E1829.
    doi: 10.3390/molecules22111829.

    PMID: 29077044

    J Cell Biochem. 2018 Feb;119(2):2231-2239.
    doi: 10.1002/jcb.26385.

    PMID: 28857247

    Phytomedicine. 2018 Feb 1;40:37-47.
    doi: 10.1016/j.phymed.2017.12.030.

    PMID: 29496173
    Calculate Dilution Ratios(Only for Reference)
    1 mg 5 mg 10 mg 20 mg 25 mg
    1 mM 1.6378 mL 8.1892 mL 16.3784 mL 32.7568 mL 40.946 mL
    5 mM 0.3276 mL 1.6378 mL 3.2757 mL 6.5514 mL 8.1892 mL
    10 mM 0.1638 mL 0.8189 mL 1.6378 mL 3.2757 mL 4.0946 mL
    50 mM 0.0328 mL 0.1638 mL 0.3276 mL 0.6551 mL 0.8189 mL
    100 mM 0.0164 mL 0.0819 mL 0.1638 mL 0.3276 mL 0.4095 mL
    * Note: If you are in the process of experiment, it's need to make the dilution ratios of the samples. The dilution data of the sheet for your reference. Normally, it's can get a better solubility within lower of Concentrations.
    Protocol
    Cell Research:
    Nat Prod Commun. 2012 Nov;7(11):1475-8.
    Neohesperidin induces cellular apoptosis in human breast adenocarcinoma MDA-MB-231 cells via activating the Bcl-2/Bax-mediated signaling pathway.[Pubmed: 23285810]
    Neohesperidin, a flavonoid compound found in high amounts in Poncirus trifoliata, has free radical scavenging activity.
    METHODS AND RESULTS:
    For the first time, our study indicated that Neohesperidin also induces cell apoptosis in human breast adenocarcinoma MDA-MB-231 cells, which was possibly mediated by regulating the P53/Bcl-2/Bax pathway. MDA-MB-231 cells were subjected to treatment with Neohesperidin. MTT and Trypan blue exclusion assays were applied to assess the cell viability. The morphological changes of cells were observed using an inverted microscope, and cell apoptosis was detected by flow cytometric analysis. Immunoblot analysis was conducted to evaluate the protein expressions of apoptosis-related genes, including P53, Bcl-2 and Bax. Our results indicated that the proliferation of MDA-MB-231 cells was inhibited by the treatment with Neohesperidin in a time- and dose-dependent manner. The IC50 values of Neohesperidin at 24 and 48 h were 47.4 +/- 2.6 microM and 32.5 +/- 1.8 microM, respectively. The expressions of P53 and Bax in the Neohesperidin-treated cells were significantly up-regulated, while that of Bcl-2 was down-regulated.
    CONCLUSIONS:
    Our study suggested that Neohesperidin could induce apoptosis of MDA-MB-231 cells, a process which was associated with the activation of the Bcl-2/Bax-mediated signaling pathway.
    Animal Research:
    Environ Toxicol Pharmacol. 2014 May;37(3):907-15.
    Effect of hesperidin and neohesperidin from bittersweet orange (Citrus aurantium var. bigaradia) peel on indomethacin-induced peptic ulcers in rats.[Pubmed: 24691249 ]
    Hesperidin and Neohesperidin are the major flavanones isolated from bittersweet orange. It was recently reported that they have potent anti-inflammatory effects in various inflammatory models.
    METHODS AND RESULTS:
    In the present study, the effects of hesperidin and Neohesperidin on indomethacin-induced ulcers in rats and the underlying mechanisms were investigated. Gastric ulcers were induced in rats with a single dose of indomethacin. The effects of pretreatment with hesperidin and Neohesperidin were assessed in comparison with omeprazole as reference standard. Ulcer index, gene expression of gastric cyclooxygenase-2 (COX-2), tumor necrosis factor alpha (TNF-α), lipid peroxidation product, malondialdhyde (MDA), and reduced glutathione (GSH) content in stomach were measured. Furthermore, gross and histopathological examination was performed.
    CONCLUSIONS:
    Our results indicated that both hesperidin and Neohesperidin significantly aggravated gastric damage caused by indomethacin administration as evidenced by increased ulcer index and histopathological changes of stomach.
    Structure Identification:
    생명과학회지, 2010,11, 1691-6.
    Optimization of Production Yield for Neohesperidin by Response Surface Methodology[Reference: WebLink]
    Neohesperidin is a natural new nutrition sweetener, widely existing in plants of dry citrus peel, which can be derived from extraction. Since the sweetness is 1,300-1,500 times greater than that of sugar, Neohesperidin are widely used in fruit juices, wines, beverages, bakeries and pharmaceutical formulations, and are particularly suitable for consumption by diabetic patients. However, the yield of extraction from citrus peel waste is very low. In this study optimal yield conditions were determinedusing response surface methodology (RSM) in order to increase the Neohesperidin extraction yield. The critical factors for maximum extraction yield were selected extraction pressure , extraction time , and concentration of ethanol . As a result, the extraction yield was improved when the extracting pressure increased. The extraction yield also increased in a time-dependent manner. When adding ethanol as an assistance solvent to the supercritical carbon dioxide, extraction yield was increased as more ethanol concentration was added. Finally, the extraction yield of Neohesperidin was improved to about 162.22% compared to ethanol extraction as a conventional method.