ChemFaces is a professional high-purity natural products manufacturer.
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2. Pharmacological research
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More articles cited ChemFaces products.
BMC Complement Altern Med.2017 Aug 9;Journal of Life Science2017.2;J. of Food Composition & Analysis2017RSC AdvancesIssue 86, 2017Front Pharmacol. 2017 Sep 29;
Inflammation. 2015 Feb 6.Front Pharmacol. 2017 Apr 25;Am J Chin Med.2016;44(8)SBRAS2016(12)Kor. J. Pharmacogn.47(1):62-72(2016)
Food and Bioprocess TechnologyJune 2017J Chromatogr Sci.2015 Feb 5. pii: bmu231. J Drug Target.2016 Feb 24:1-28.Biochem Biophys Res Commun. 2018 Jan 1;
Our products had been exported to the following research institutions and universities, And still growing.
Center for protein Engineering (... (Belgium)University of Fribourg (Switzerland)Massachusetts General Hospital (USA)University of Illinois at Chicago (USA)
Universita' Degli Studi Di Cagli... (Italy)Leibniz Institute of Plant Bioch... (Germany)University of Hawaii Cancer Center (USA)Centrum Menselijke Erfelijkheid (Belgium)
University of Melbourne (Australia)University of Queensland (Australia)University of Minnesota (USA)
||Onjisaponin B induces autophagy via the AMPK-mTOR signaling pathway, increases the NGF level and accelerates both the removal of mutant huntingtin and A53T α-synuclein, it may have potential therapeutic effects on Parkinson disease, Alzheimer disease and Huntington disease. Onjisaponins may provide safe and potent adjuvants for intranasal inoculation of influenza HA and DPT vaccines.
||AMPK | mTOR | PGE | TNF-α | NGF|
|Int J Mol Sci. 2013 Nov 15;14(11):22618-41. |
|Onjisaponin B derived from Radix Polygalae enhances autophagy and accelerates the degradation of mutant α-synuclein and huntingtin in PC-12 cells.[Pubmed: 24248062]|
|Emerging evidence indicates important protective roles being played by autophagy in neurodegenerative disorders through clearance of aggregate-prone or mutant proteins. |
METHODS AND RESULTS:
In the current study, we aimed to identify autophagy inducers from Chinese medicinal herbs as a potential neuroprotective agent that enhances the clearance of mutant huntingtin and α-synuclein in PC-12 cells. Through intensive screening using the green fluorescent protein-light chain 3 (GFP-LC3) autophagy detection platform, we found that the ethanol extracts of Radix Polygalae (Yuan Zhi) were capable of inducing autophagy. Further investigation showed that among three single components derived from Radix Polygalae--i.e., polygalacic acid, senegenin and Onjisaponin B--Onjisaponin B was able to induce autophagy and accelerate both the removal of mutant huntingtin and A53T α-synuclein, which are highly associated with Huntington disease and Parkinson disease, respectively. Our study further demonstrated that Onjisaponin B induces autophagy via the AMPK-mTOR signaling pathway.
Therefore, findings in the current study provide detailed insights into the protective mechanism of a novel autophagy inducer, which is valuable for further investigation as a new candidate agent for modulating neurodegenerative disorders through the reduction of toxicity and clearance of mutant proteins in the cellular level.
|Biomed Res Int. 2015;2015:918048. |
|The gastrointestinal irritation of polygala saponins and its potential mechanism in vitro and in vivo.[Pubmed: 25705699]|
|Processing alters the pharmacological activity and reduces the gastrointestinal toxicity of the polygalae.|
METHODS AND RESULTS:
To investigate the effect of processing, different glycosyl substituent products were tested. Hypnotic and subhypnotic doses of pentobarbital-induced sleep tests on mice were used to evaluate the sedative activity of polygala saponins with different glycosyl substituents; isolated gut motility experiment was employed to study excitatory effects of different polygala saponins; the gastrointestinal irritation effects of different polygala saponins were compared by measuring the levels of gastric PGE2 and intestinal TNF-α on mice. When compared with control, Onjisaponin B (OJB) and tenuifolin (TEN), but not senegenin (SNG), significantly increased the number of sleeping mice and prolonged the sleeping time (P < 0.05); 80, 40, and 20 mg/L of OJB and 80 mg/L of TEN, but not SNG, obviously changed the amplitude and frequency of isolated jejunum (P < 0.05); all the three compounds significantly decreased the level of gastric PGE2 but had no obvious influences on the reduction of intestinal TNF-α level. For sedative and hypnotic effects, OJB > TEN > SNG; for the protection form gastrointestinal irritation and damages, OJB > TEN > SNG.
Therefore, in processing Polygala, glycosyl breaking may be related to the decline of pharmacological activity and gastrointestinal toxicity of polygala saponins.
Onjisaponin B Description
||The roots of Polygala tenuifolia.
||DMSO, Pyridine, Methanol, Ethanol, etc.
||Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please email to: firstname.lastname@example.org
||The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
Recent ChemFaces New Products and Compounds
Recently, ChemFaces products have been cited in many studies from excellent and top scientific journals
Cell. 2018 Jan 11;172(1-2):249-261.e12. doi: 10.1016/j.cell.2017.12.019.PMID: 29328914
Mol Cell. 2017 Nov 16;68(4):673-685.e6. doi: 10.1016/j.molcel.2017.10.022.PMID: 29149595
Scientific Reports 2017 Dec 11;7(1):17332.doi: 10.1038/s41598-017-17427-6.PMID: 29230013
Molecules. 2017 Oct 27;22(11). pii: E1829.doi: 10.3390/molecules22111829.PMID: 29077044
J Cell Biochem. 2018 Feb;119(2):2231-2239.doi: 10.1002/jcb.26385. PMID: 28857247
Phytomedicine. 2018 Feb 1;40:37-47. doi: 10.1016/j.phymed.2017.12.030.PMID: 29496173
Calculate Dilution Ratios(Only for Reference)
* Note: If you are in the process of experiment, it's need to make the dilution ratios of the samples. The dilution data of the sheet for your reference. Normally, it's can get a better solubility within lower of Concentrations.