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    Otophylloside O
    Otophylloside O
    Information
    CAS No. 1326583-08-7 Price
    Catalog No.CFN89036Purity>=98%
    Molecular Weight1077.25Type of CompoundSteroids
    FormulaC56H84O20Physical DescriptionPowder
    Download     COA    MSDSSimilar structuralComparison (Web)
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    Size /Price /Stock 10 mM * 1 mL in DMSO / Inquiry
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    Description: Otophylloside O is a natural product from Cynanchum otophyllum.
    Otophylloside O Description
    Source: The roots of Cynanchum otophyllum.
    Solvent: Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.
    Storage: Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Need more advice on solubility, usage and handling? Please email to: service@chemfaces.com

    After receiving: The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
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    Cell. 2018 Jan 11;172(1-2):249-261.e12.
    doi: 10.1016/j.cell.2017.12.019.
    IF=36.216(2019)

    PMID: 29328914

    Cell Metab. 2020 Mar 3;31(3):534-548.e5.
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    IF=22.415(2019)

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    IF=14.548(2019)

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    IF=13.903(2019)

    PMID: 29553709

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    doi: 10.1038/nplants.2016.205.
    IF=13.297(2019)

    PMID: 28005066

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    PMID: 30417089
    Calculate Dilution Ratios(Only for Reference)
    1 mg 5 mg 10 mg 20 mg 25 mg
    1 mM 0.9283 mL 4.6414 mL 9.2829 mL 18.5658 mL 23.2072 mL
    5 mM 0.1857 mL 0.9283 mL 1.8566 mL 3.7132 mL 4.6414 mL
    10 mM 0.0928 mL 0.4641 mL 0.9283 mL 1.8566 mL 2.3207 mL
    50 mM 0.0186 mL 0.0928 mL 0.1857 mL 0.3713 mL 0.4641 mL
    100 mM 0.0093 mL 0.0464 mL 0.0928 mL 0.1857 mL 0.2321 mL
    * Note: If you are in the process of experiment, it's need to make the dilution ratios of the samples. The dilution data of the sheet for your reference. Normally, it's can get a better solubility within lower of Concentrations.
    Protocol
    Structure Identification:
    Zhongguo Zhong Yao Za Zhi. 2014 Apr;39(8):1450-6.
    C21 steroids from roots of Cynanchum otophyllum.[Pubmed: 25039181]
    Eleven C21 steroids were isolated from chloroform extract of roots of Cynanchum otophyllumby silica gel, MCI, ODS columns, and semi-preparative HPLC.
    METHODS AND RESULTS:
    Their structures were determined by spectroscopic data analysis as otophylloside B(1), caudatin-3-O-beta-D-cymaropyranosyl-(1-->4)-beta-D-oleandropyranosyl-(1-->4)-beta-D-cymaropyranosyl-(1-->4)-beta-D-cymaropyranoside (2), caudatin-3-O-beta-D-oleandropyranosyl-(1-->4)-beta-D-oleandropyranosyl-(1-->4)-beta-D-cymaropyranosyl-(1-->4)-beta-D-cymaropyranoside (3), caudatin-3-O-beta-D-oleandropyranosyl-(1-->4)-beta-D-digitoxopyranosyl-(1-->4)-beta-D-cymaropyranoside (4), Otophylloside O (5), gagamine-3-O-beta-D-oleandropyranosyl-(1-->4)-beta-D-cymaropyranosyl-(1-->4)-beta-D-cymaropyranoside (6), sinomarinoside B (7), mucronatosides C (8), wallicoside J (9), stephanoside H (10), and qinyangshengenin-3-O-beta-D-oleandropyranosyl-(1-->4)-beta-D-cymaropyranosyl-(1-->4)-beta-D-digitoxopyranoside (11).
    CONCLUSIONS:
    Among them, compounds 2-3, and 6-11 were separated from the roots of this plant for the first time.
    Steroids. 2011 Sep-Oct;76(10-11):1003-9.
    Identification of new qingyangshengenin and caudatin glycosides from the roots of Cynanchum otophyllum.[Pubmed: 21513722 ]

    METHODS AND RESULTS:
    HPLC analysis of the roots of Cynanchum otophyllum Scheind (Asclepiadaceae) led to the isolation of six new pregnane glycosides, specifically otophylloside N, Otophylloside O, otophylloside P (2-4) and otophyllosides Q-S (7-9), in addition to the identification of three known C-21 steroidal glycosides, otophylloside A (1), otophylloside B (5) and caudatin 3-O-β-D-glucopyranosyl-(1→4)-β-D-oleandropyranosyl-(1→4)-β-D-cymaropyranosyl-(1→4)-β-D-cymaropyranoside (6). The structure of each glycoside was determined by detailed spectroscopic analysis and chemical methods.
    CONCLUSIONS:
    All compounds contain qingyangshengenin or caudatin aglycones and a straight sugar chain consisting of 4-7 hexosyl moieties with the mode of 1→4 linkage. The optically isomeric monosaccharides, D- and L-cymarose, coexisted in both otophyllosides R (8) and S (9).