Info: Read More
  • ChemFaces is a professional high-purity natural products manufacturer.
  • Product Intended Use
  • 1. Reference standards
  • 2. Pharmacological research
  • 3. Inhibitors
  •   
  • Home
  • Natural Products
  • Bioactive
  • Screening Libraries
  • Hot Products
  • Plant Catalog
  • Customer Support
  • Product Use Citation
  • About Us
  • Contact Us
  • Science | Nature | Cell | View More
    Natural Products
    Physalin H
    Information
    CAS No. 70241-09-7 Price
    Catalog No.CFN92937Purity>=98%
    Molecular Weight562.99Type of CompoundSteroids
    FormulaC28H31ClO10Physical DescriptionPowder
    Download     COA    MSDSSimilar structuralComparison (Web)
    Citing Use of our Products
    How to Order
    Orders via your E-mail:

    1. Product number / Name / CAS No.
    2. Delivery address
    3. Ordering/billing address
    4. Contact information
    Sent to Email: info@chemfaces.com
    Contact Us
    Order & Inquiry & Tech Support

    Tel: (0086)-27-84237683
    Fax: (0086)-27-84254680
    E-mail: manager@chemfaces.com
    Address: No. 83, CheCheng Rd., WETDZ, Wuhan, Hubei 430056, PRC
    Delivery time
    Delivery & Payment method

    1. Usually delivery time: Next day delivery by 9:00 a.m. Order now

    2. We accept: Wire transfer & Credit card & Paypal & Western Union
    * Packaging according to customer requirements(5mg, 10mg, 20mg and more). We shipped via FedEx, DHL, UPS, EMS and others courier.
    According to end customer requirements, ChemFaces provide solvent format. This solvent format of product intended use: Signaling Inhibitors, Biological activities or Pharmacological activities.
    Size /Price /Stock 10 mM * 1 mL in DMSO / Inquiry
    Other Packaging *Packaging according to customer requirements(100uL/well, 200uL/well and more), and Container use Storage Tube With Screw Cap
    Our products had been exported to the following research institutions and universities, And still growing.
  • National Cancer Institute (USA)
  • Universidade Federal de Goias (... (Brazil)
  • Uniwersytet Jagielloński w Kra... (Poland)
  • National Research Council of Ca... (Canada)
  • Universidad de La Salle (Mexico)
  • Monash University (Australia)
  • S.N.D.T. Women's University (India)
  • Mendel University in Brno (Czech Republic)
  • University of Leipzig (Germany)
  • Universidad de Buenos Aires (Argentina)
  • Charles Sturt University (Denmark)
  • More...
  • Package
    Featured Products
    Luteolin-7-O-glucoside

    Catalog No: CFN98565
    CAS No: 5373-11-5
    Price: $60/20mg
    Arteannuin B

    Catalog No: CFN98807
    CAS No: 50906-56-4
    Price: $228/20mg
    Tangeretin

    Catalog No: CFN90240
    CAS No: 481-53-8
    Price: $30/20mg
    Sanggenone K

    Catalog No: CFN92416
    CAS No: 86450-77-3
    Price: $533/5mg
    Dihydromorin

    Catalog No: CFN99854
    CAS No: 18422-83-8
    Price: $403/5mg
    4-O-Feruloylquinic acid

    Catalog No: CFN92392
    CAS No: 2613-86-7
    Price: $448/5mg
    cis-Mulberroside A

    Catalog No: CFN95006
    CAS No: 166734-06-1
    Price: $418/10mg
    Related Screening Libraries
    Size /Price /Stock 10 mM * 100 uL in DMSO / Inquiry / In-stock
    10 mM * 1 mL in DMSO / Inquiry / In-stock
    Related Libraries
  • Steroids Compound Library
  • HO-1 Inhibitor Library
  • gp120/CD4 Inhibitor Library
  • Antifection Inhibitor Library
  • Biological Activity
    Description: Physalin H is an Hh signaling inhibitor blocks GLI1-DNA-complex formation, it also strong quinone reductase induction activity with IR (Induction ratio, QR induction activity) value of 3.74±0.02. Physalin H shows immunosuppressive effects on T cells both in vitro and in vivo, and the immunosuppressive activity might be attributed to the suppression of T cell activation and proliferation, the modulation of Th1/Th2 cytokine balance and the induction of HO-1 in T cells. Physalin H shows significant in vitro leishmanicidal activities (0.92-19.4 microg/ml) against promastigotes of Leishmania major.Physalin H shows cytotoxic activity against a panel of human and murine cancer cell lines.
    Targets: HO-1 | gp120/CD4 | Antifection
    In vitro:
    Steroids. 2014 Oct;88:60-5.
    Unprecedent aminophysalin from Physalis angulata.[Pubmed: 24973634 ]

    METHODS AND RESULTS:
    The 95% ethanol extract of the whole plant of Physalis angulata Linn. afforded one new skeletal physalin named aminophysalin A (1) and one new naturally occurring 5β-hydroxy-6a-chloro-5,6-dihydrophysalin B (2), together with five known physalins (3-7). Their structures were elucidated through MS, IR, NMR spectroscopy analyses and X-ray crystallography. Aminophysalin A (1) had an absolutely unusual structural feature in the chemistry of physalins with a nitrogen atom. Compounds 1-7 were evaluated for quinone reductase activities in hepa 1c1c7 cells.
    CONCLUSIONS:
    Physalin H (6) showed strong quinone reductase induction activity with IR (Induction ratio, QR induction activity) value of 3.74±0.02, using 4-bromoflavone as a positive control substance (2.17±0.01, 10 μg/mL), while compounds 1, 2, 3, 5 showed weak quinone reductase induction activity.
    Beilstein J Org Chem. 2014 Jan 13;10:134-40.
    Physalin H from Solanum nigrum as an Hh signaling inhibitor blocks GLI1-DNA-complex formation.[Pubmed: 24454566 ]
    Hedgehog (Hh) signaling plays an important role in embryonic development, cell maintenance and cell proliferation. Moreover, Hh signaling contributes to the growth of cancer cells. Physalins are highly oxidized natural products with a complex structure.
    METHODS AND RESULTS:
    Physalins (1-7) were isolated from Solanum nigrum (Solanaceae) collected in Bangladesh by using our cell-based assay. The isolated physalins included the previously reported Hh inhibitors 5 and 6. Compounds 1 and 4 showed strong inhibition of GLI1 transcriptional activity, and exhibited cytotoxicity against cancer cell lines with an aberrant activation of Hh signaling. Compound 1 inhibited the production of the Hh-related proteins patched (PTCH) and BCL2.
    CONCLUSIONS:
    Analysis of the structures of different physalins showed that the left part of the physalins was important for Hh inhibitory activity. Interestingly, Physalin H (1) disrupted GLI1 binding to its DNA binding domain, while the weak inhibitor physalin G (2) did not show inhibition of GLI1-DNA complex formation.
    Chem Biodivers. 2005 Sep;2(9):1164-73.
    Antileishmanial physalins from Physalis minima.[Pubmed: 17193198 ]

    METHODS AND RESULTS:
    Three new physalins (1-3) and a new withanolide 7 have been isolated from the whole plant of Physalis minima, along with three known physalins: Physalin H (4), isophysalin B (5), and 5beta,6beta-epoxyphysalin B (6). Their structures were deduced on the basis of in-depth spectroscopic analyses.
    CONCLUSIONS:
    Compounds 1-6 showed significant in vitro leishmanicidal activities (0.92-19.4 microg/ml) against promastigotes of Leishmania major.
    In vivo:
    Int Immunopharmacol. 2010 Mar;10(3):290-7.
    Investigation of the immunosuppressive activity of Physalin H on T lymphocytes.[Pubmed: 19951747]
    Physalis angulata is an annual herb widely used in folk medicine. It is mainly used for treating rheumatoid arthritis (RA). Following bioactivity-guided isolation, a representative immunosuppressive compound, Physalin H was been identified from this herb medicine. The purpose of this work was to assess the immunosuppressive activity of Physalin H on T cells and to explore its potential mode of action.
    METHODS AND RESULTS:
    The results showed that Physalin H in a dose-dependent manner significantly inhibited the proliferation of T cells induced by concanavalin A (ConA) and by the mixed lymphocyte culture reaction (MLR). This inhibitive activity was mainly due to interfering DNA replication in G1 stages. In vivo experiments showed that, administration of Physalin H dose-dependently suppressed CD4(+) T cell mediated delayed-type hypersensitivity (DTH) reactions, and suppressed antigen-specific T-cell response in ovalbumin (OVA) immunized mice. Further study indicated that Physalin H could modulate Th1/Th2 cytokine balance and induce the production of immune regulation target Heme oxygenase (HO)-1 in T-cells in vitro.
    CONCLUSIONS:
    In this study, we demonstrated the immunosuppressive effect of Physalin H on T cells both in vitro and in vivo, and the immunosuppressive activity might be attributed to the suppression of T cell activation and proliferation, the modulation of Th1/Th2 cytokine balance and the induction of HO-1 in T cells.
    Physalin H Description
    Source: The herbs of Physalis alkekengi.
    Solvent: Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.
    Storage: Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Need more advice on solubility, usage and handling? Please email to: service@chemfaces.com

    After receiving: The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
    Recent ChemFaces New Products and Compounds
    Hinokiflavone

    Catalog No: CFN92007
    CAS No: 19202-36-9
    Price: $238/10mg
    Kaempferol 3-O-beta-(6''-p-coumaro...

    Catalog No: CFN95089
    CAS No: 111957-48-3
    Price: $338/10mg
    Safflospermidine B

    Catalog No: CFN95254
    CAS No: N/A
    Price: $413/5mg
    Polygalin C

    Catalog No: CFN95118
    CAS No: 934768-05-5
    Price: $288/5mg
    Kobophenol A

    Catalog No: CFN92530
    CAS No: 124027-58-3
    Price: $318/10mg
    Glycycoumarin

    Catalog No: CFN89408
    CAS No: 94805-82-0
    Price: $268/10mg
    Fargesol

    Catalog No: CFN95027
    CAS No: 128855-64-1
    Price: $318/20mg
    N-trans-caffeoyloctopamine

    Catalog No: CFN95264
    CAS No: 1378868-10-0
    Price: $413/5mg
    Recently, ChemFaces products have been cited in many studies from excellent and top scientific journals

    Cell. 2018 Jan 11;172(1-2):249-261.e12.
    doi: 10.1016/j.cell.2017.12.019.
    IF=36.216(2019)

    PMID: 29328914

    Cell Metab. 2020 Mar 3;31(3):534-548.e5.
    doi: 10.1016/j.cmet.2020.01.002.
    IF=22.415(2019)

    PMID: 32004475

    Mol Cell. 2017 Nov 16;68(4):673-685.e6.
    doi: 10.1016/j.molcel.2017.10.022.
    IF=14.548(2019)

    PMID: 29149595

    ACS Nano. 2018 Apr 24;12(4): 3385-3396.
    doi: 10.1021/acsnano.7b08969.
    IF=13.903(2019)

    PMID: 29553709

    Nature Plants. 2016 Dec 22;3: 16206.
    doi: 10.1038/nplants.2016.205.
    IF=13.297(2019)

    PMID: 28005066

    Sci Adv. 2018 Oct 24;4(10): eaat6994.
    doi: 10.1126/sciadv.aat6994.
    IF=12.804(2019)

    PMID: 30417089
    Calculate Dilution Ratios(Only for Reference)
    1 mg 5 mg 10 mg 20 mg 25 mg
    1 mM 1.7762 mL 8.8812 mL 17.7623 mL 35.5246 mL 44.4058 mL
    5 mM 0.3552 mL 1.7762 mL 3.5525 mL 7.1049 mL 8.8812 mL
    10 mM 0.1776 mL 0.8881 mL 1.7762 mL 3.5525 mL 4.4406 mL
    50 mM 0.0355 mL 0.1776 mL 0.3552 mL 0.7105 mL 0.8881 mL
    100 mM 0.0178 mL 0.0888 mL 0.1776 mL 0.3552 mL 0.4441 mL
    * Note: If you are in the process of experiment, it's need to make the dilution ratios of the samples. The dilution data of the sheet for your reference. Normally, it's can get a better solubility within lower of Concentrations.
    Protocol
    Cell Research:
    Phytother Res. 2003 May;17(5):520-3.
    Cytotoxic constituents of Brachistus stramoniifolius.[Pubmed: 12748990 ]
    Phytochemical investigation of Brachistus stramoniifolius (Kunth) Miers (formerly Witheringia stramoniifolia Kunth) (Solanaceae) was initiated following primary biological screening and in view of the absence of prior phytochemical studies conducted on this species.
    METHODS AND RESULTS:
    Fractionation of an ethyl acetate extract from the roots of the plant, guided by in vitro cytotoxic activity using cultured KB (human nasopharyngeal carcinoma) cells, led to the isolation of three known compounds of the 13,14-seco-16,24-cyclosteroid type, physalin B (1), physalin F (2) andPhysalin H (3). Their structures were characterized by comparison of their physical and spectral data to published values, with 1D- and 2D-NMR experiments being performed to assure unambiguous resonance assignments.
    CONCLUSIONS:
    Biological evaluation of these three compounds against a panel of human and murine cancer cell lines demonstrated their broad cytotoxic activity.