|Description:|| 1. Quercetin 3,4’-dimethyl ether shows anti-lipid peroxidation activity (IC 50 values of 0.3 uM).|
2. 5,7,3'-Trihydroxy-3,4'-dimethoxyflavone (Quercetin 3,4’-dimethyl ether ) has high cytotoxic against leukemia cells, it induces cell death is mediated by an intrinsic dependent apoptotic event involving mitochondria and MAPKs, and through a mechanism independent of the generation of reactive oxygen species, suggests that it could be useful in the development of novel anticancer agents.
3. Quercetin 3,4’-dimethyl ether shows anti-inflammatory activity.
|Targets:||Bcl-2/Bax | Caspase | JNK | ERK | MAPK|
|Source:||The branch of Tamarix chinensis Lour.|
|Solvent:||Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.|
|Storage:||Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
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|After receiving:||The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.|
|1 mg||5 mg||10 mg||20 mg||25 mg|
|1 mM||3.0276 mL||15.1378 mL||30.2755 mL||60.551 mL||75.6888 mL|
|5 mM||0.6055 mL||3.0276 mL||6.0551 mL||12.1102 mL||15.1378 mL|
|10 mM||0.3028 mL||1.5138 mL||3.0276 mL||6.0551 mL||7.5689 mL|
|50 mM||0.0606 mL||0.3028 mL||0.6055 mL||1.211 mL||1.5138 mL|
|100 mM||0.0303 mL||0.1514 mL||0.3028 mL||0.6055 mL||0.7569 mL|
Arch.Pharm.Res., 2003, 26(7):535-9.
|Antioxidant constituents from the stem of Sorghum bicolor.[Pubmed: 12934645]|
|The EtOAc soluble fraction from the stem of Sorghum bicolor showed a strong free radical scavenging activity. Five major compounds were isolated from this fraction. They were identified by spectral data as methyl ferulate (1), methyl p-hydroxycinnamate (2), p-hydroxybenzaldehyde (3), tricin (4), and Quercetin 3,4'-dimethyl ether (5). Among these compounds, 1 exhibited a strong, free radical scavenging activity on 1,1-diphenyl-2-picrylhydrazyl (DPPH) with an IC50 value of 0.7 microM. We further studied the effects of these isolated compounds on the lipid peroxidation in rat liver microsomes induced by non-enzymatic method. All five compounds showed anti-lipid peroxidation activity (IC50 values of 0.5, 0.4, 0.3 and 0.3 microM, respectively).|
Mol. Carcinogen., 2010, 49(5): 464-75.
|5,7,3'-trihydroxy-3,4'-dimethoxyflavone-induced cell death in human leukemia cells is dependent on caspases and activates the MAPK pathway.[Pubmed: 20175127]|
|Flavonoids are polyphenolic compounds which display a vast array of biological activities and are promising anticancer agents. In this study we investigated the effect of 5,7,3'-trihydroxy-3,4'-dimethoxyflavone (Quercetin 3,4'-dimethyl ether,THDF) on viability of nine human tumor cell lines and found that it was highly cytotoxic against leukemia cells. THDF induced G(2)-M phase cell-cycle arrest and apoptosis through a caspase-dependent mechanism involving cytochrome c release, processing of multiple caspases (caspase-3, -6, -7, and -9) and cleavage of poly(ADP-ribose) polymerase. Overexpression of the protective mitochondrial proteins Bcl-2 and Bcl-x(L) conferred partial resistance to THDF-induced apoptosis. This flavonoid induced the phosphorylation of members of the mitogen-activated protein kinases (MAPKs) family and cell death was attenuated by inhibition of c-jun N-terminal kinases/stress-activated protein kinases (JNK/SAPK) and of extracellular signal-regulated kinases (ERK) 1/2.|