|《China Pharmacy》 2012-08 |
|Isolation and Identification of Salvianolic Acid D and Simultaneous Determination of 4 Salvianolic Acids in Danshen Infusion[Reference: WebLink]|
|To establish the method for the isolation and identification of Salvianolic acid D and simultaneous determination of tanshinol,protocatechuic aldehyde,Salvianolic acid D and B in Danshen infusion.|
METHODS AND RESULTS:
RP-HPLC method was adopted for isolation and content determination.The determination was performed on Kromasil C18 column with mobile phase consisted of acetonitrile-1% glacial acetic acid at the flow rate of 1.0 mL·min-1.The injection volume was 10 μL and column temperature was 30 ℃.The detection wavelength was set at 281 nm. Salvianolic acid D had been succeddfully isolated and indentified.The linearities of tanshinol,protocatechuic aldehyde,Salvianolic acid D,salvianolic acid B were 60.9～975.0,8.7～139.0,11.0～175.8,2.7～43.2 μg·mL-1.The average recoveries rate were 99.1%,100.2%,98.3%,100.4%,102.5%,101.1%,101.4%,97.0%,102.1%,100.6%,99.2%,101.3%,and the RSDs were 0.94%,0.97%,2.78%,1.07%.
The method is simple,rapid and specific.It may be suitable for the quality control of Danshen infusion.