ChemFaces is a professional high-purity natural products manufacturer.
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2. Pharmacological research
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More articles cited ChemFaces products.
Front Pharmacol. 2016 Nov 30Naunyn Schmiedebergs Arch Pharmacol. 2017 Jul 21. Journal of Analytical ChemistryAug. 2017;Front Pharmacol. 2017 Sep 29;Pharmacogn Mag.2015 Jul-Sep.
Journal of Medicinal Plant Research.2013 Nov 7Aquaculture1 Dec. 2017JPCJuly 12, 2017Clin Exp Pharmacol Physiol.2015 Aug 1Biomed Chromatogr. 2016 Mar 23.
Acta Agriculturae ScandinavicaJan. 18, 2016Br J Pharmacol.2018 Mar;Evidence-Based Comp. & Alter. Med.Dec. 27, 2015J Pharm Biomed Anal. 2017 Jun 5;
Our products had been exported to the following research institutions and universities, And still growing.
Regional Crop Research Institute (Korea)Shanghai Institute of Organic Ch... (China)Utrecht University (Netherlands)University Medical Center Mainz (Germany)
National Hellenic Research Found... (Greece)Research Unit Molecular Epigenet... (Germany)Chungnam National University (Korea)University of Melbourne (Australia)
Monash University Sunway Campus (Malaysia)Uniwersytet Medyczny w ?odzi (Poland)Chulalongkorn University (Thailand)
||Silychristin is a plant growth regulator, is an anti-hepatotoxic agent, is also an inhibitor of horseradish peroxidases and lipoxygenase.|
||NADPH-oxidase | P450 (e.g. CYP17)|
|ongress of the Turkish Toxicology Society. 2015,10. |
|In vitro assessment of human CYP1A1 inhibition potential of Resveratrol and Silychristin[Reference: WebLink]|
|Flavonoids are phenolic compounds with low molecule weight, which are found most plants in nature. They are so important for human health due to their biological activities such as anti-inflammatory and anti-carcinogenic effects. Cancer protective effects of flavonoids have been attributed to wide variety of mechanisms such as free radical scavenging and modifying Phase I and Phase II enzymes that activate or detoxify carcinogens. Resveratrol and Silychristin are phenolic compounds that may have roles in the reduction of cancer susceptibility. One of the possible mechanism by which resveratrol and Silychristin may exert their anti-carcinogenic effects is through an interaction by certain CYP450s. |
METHODS AND RESULTS:
In this respect, the focus of this study is to determine the mechanisms of inhibition of CYP1A1, that is known to be involved in the activation of procarcinogens by resveratrol and Silychristin. Bistronic expression system that coexpress human CYP1A1 and NADPH CYP450 Reductase were used to investigate this effect. Co-expression plasmid was transformed into E. coli DH5alpha. Single colony was selected and grown in overnight culture at 30°C in LB medium. Membrane fractions were prepared and used for enzyme source. Resveratrol inhibited ethoxyresorufin O-deethylation (EROD) activity in human P450 1A1 in a dose-dependent manner with IC50 of 21 μM. Moreover, resveratrol inhibited human P450 1A1 activity in a mixed-type inhibition. In the case of Silychristin, the inhibition of human P450 1A1 by this phenolic compound was stronger than resveratrol. (IC50 15.83 μM for EROD). Similiarly, it showed mixed type inhibition.
This study indicated that these phenolics were strong and selective inhibitors of CYP1A1 associated EROD activity and may be considered for use as a strong cancer chemopreventive agent in humans by the preventing malignant transformation and reducing the activations of carcinogens through inhibition of CYP1A1.
||The herbs of Silybum marianum (L.) Gaertn.
||Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.
||Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please email to: firstname.lastname@example.org
||The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
Recent ChemFaces New Products and Compounds
Recently, ChemFaces products have been cited in many studies from excellent and top scientific journals
Cell. 2018 Jan 11;172(1-2):249-261.e12. doi: 10.1016/j.cell.2017.12.019.PMID: 29328914
Mol Cell. 2017 Nov 16;68(4):673-685.e6. doi: 10.1016/j.molcel.2017.10.022.PMID: 29149595
Scientific Reports 2017 Dec 11;7(1):17332.doi: 10.1038/s41598-017-17427-6.PMID: 29230013
Molecules. 2017 Oct 27;22(11). pii: E1829.doi: 10.3390/molecules22111829.PMID: 29077044
J Cell Biochem. 2018 Feb;119(2):2231-2239.doi: 10.1002/jcb.26385. PMID: 28857247
Phytomedicine. 2018 Feb 1;40:37-47. doi: 10.1016/j.phymed.2017.12.030.PMID: 29496173
Calculate Dilution Ratios(Only for Reference)
* Note: If you are in the process of experiment, it's need to make the dilution ratios of the samples. The dilution data of the sheet for your reference. Normally, it's can get a better solubility within lower of Concentrations.
|Int J Mol Sci. 2015 May 26;16(6):11983-95. |
|Regioselective alcoholysis of silychristin acetates catalyzed by lipases.[Pubmed: 26016503]|
|A panel of lipases was screened for the selective acetylation and alcoholysis of Silychristin and Silychristin peracetate, respectively.
METHODS AND RESULTS:
Acetylation at primary alcoholic group (C-22) of Silychristin was accomplished by lipase PS (Pseudomonas cepacia) immobilized on diatomite using vinyl acetate as an acetyl donor, whereas selective deacetylation of 22-O-acetyl Silychristin was accomplished by Novozym 435 in methyl tert-butyl ether/ n-butanol. Both of these reactions occurred without diastereomeric discrimination of Silychristin A and B.
Both of these enzymes were found to be capable to regioselective deacetylation of hexaacetyl Silychristin to afford penta-, tetra- and tri-acetyl derivatives, which could be obtained as pure synthons for further selective modifications of the parent molecule.