ChemFaces is a professional high-purity natural products manufacturer.
Product Intended Use
1. Reference standards
2. Pharmacological research
How to Order
Orders via your E-mail:
1. Product number / Name / CAS No.
2. Delivery address
3. Ordering/billing address
4. Contact information
Sent to Email: email@example.com
Order & Inquiry & Tech Support
Address: No. 83, CheCheng Rd., WETDZ, Wuhan, Hubei 430056, PRC
Delivery & Payment method
1. Usually delivery time: Next day delivery by 9:00 a.m. Order now
2. We accept: Wire transfer & Credit card & Paypal & Western Union
* Packaging according to customer requirements(5mg, 10mg, 20mg and more). We shipped via FedEx, DHL, UPS, EMS and others courier.
More articles cited ChemFaces products.
Biochem Pharmacol. 2017 Apr 15;J Drug Target.2016 Feb 24:1-28.British Jou. Med. & Med. Research2014 Jan 1Phytochem Anal.2016 Sep;University of Central Lancashire2017;
Evid Based Complement Alternat Med. 2016 May 24; Int J Mol Sci. 2017 Dec 21;Food Science and Bio.31 October 2016Pharm Biol. 2017 Dec;Universidade Estadual Paulista2017;
Evid Based Complement Alternat Med. 2017:6360836.Acta Physiologiae Plantarum.2014 10.J Pharm Biomed Anal. 2016 Jun 25;129:50-59. Biochem Biophys Res Commun. 2017 Dec 16;
Our products had been exported to the following research institutions and universities, And still growing.
Max Rubner-Institut (MRI) (Germany)Siksha O Anusandhan University (India)Florida A&M University (USA)Julius Kühn-Institut (Germany)
Nicolaus Copernicus Uniwersity (Poland)Chinese University of Hong Kong (China)Medical University of Gdansk (Poland)Colorado State University (USA)
Instytut Nawozów Sztucznych w P... (Poland)Center for protein Engineering (... (Belgium)Ain Shams University (Egypt)
||Sinapine is an alkaloid from seeds of the cruciferous species which shows favorable biological activities such as antioxidant and radio-protective activities.|
||P-gp | ERK|
|Food Chem Toxicol. 2014 May;67:187-92. |
|Sinapine as an active compound for inhibiting the proliferation of Caco-2 cells via downregulation of P-glycoprotein.[Pubmed: 24607798]|
|Sinapine, an alkaloid from seeds of the cruciferous species, shows favorable biological activities such as antioxidant and radio-protective activities. However, the inhibitory effect of Sinapine on tumors, and the molecular mechanisms have not been completely understood thus far. In this study, we determined anti-proliferative effects of Sinapine.
METHODS AND RESULTS:
We examined the anti-tumor effects of the combination of Sinapine and doxorubicin. The results of the MTT assay and apoptosis showed that Sinapine increased the sensitivity of Caco-2 cells to doxorubicin in a dose-dependent manner, whereas no or less effect was observed in the cells treated with doxorubicin alone. The combination of Sinapine and doxorubicin had a synergistic effect and increased the cytotoxicity of doxorubicin against Caco-2 cells. Doxorubicin accumulation assay showed that Sinapine increased the intracellular accumulation of doxorubicin in dose-dependent manner. Immunoblotting and QT-PCR analysis showed that Sinapine suppressed P-glycoprotein (P-gp) expression via ubiquitination. A significant correlation was observed between the expression of p-ERK1/2 and P-gp.
These results indicated that Sinapine played an important role in the down-regulation of P-gp expression through suppression of FGFR4-FRS2α-ERK1/2 signaling pathway. To our knowledge, this is the first study to show that Sinapine can be used as an effective natural compound for chemo-resistance.
|PLoS One. 2015 Jan 21;10(1):e0116470. |
|Characterization of the factors that influence sinapine concentration in rapeseed meal during fermentation.[Pubmed: 25606856]|
|We analyzed and compared the difference in Sinapine concentration in rapeseed meal between the filamentous fungus, Trametes sp 48424, and the yeast, Saccharomyces cerevisiae, in both liquid and solid-state fermentation.
METHODS AND RESULTS:
During liquid and solid-state fermentation by Trametes sp 48424, the Sinapine concentration decreased significantly. In contrast, the liquid and solid-state fermentation process by Saccharomyces cerevisiae just slightly decreased the Sinapine concentration (P ≤ 0.05). After the solid-state fermented samples were dried, the concentration of Sinapine in rapeseed meal decreased significantly in Saccharomyces cerevisiae. Based on the measurement of laccase activity, we observed that laccase induced the decrease in the concentration of Sinapine during fermentation with Trametes sp 48424. In order to eliminate the influence of microorganisms and the metabolites produced during fermentation, high moisture rapeseed meal and the original rapeseed meal were dried at 90 °C and 105 °C, respectively. During drying, the concentration of Sinapine in high moisture rapeseed meal decreased rapidly and we obtained a high correlation coefficient between the concentration of Sinapine and loss of moisture.
Our results suggest that drying and enzymes, especially laccase that is produced during the solid-state fermentation process, may be the main factors that affect the concentration of Sinapine in rapeseed meal.
||The seeds of Raphanus sativus L.
||Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.
||Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please email to: firstname.lastname@example.org
||The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
Recent ChemFaces New Products and Compounds
Recently, ChemFaces products have been cited in many studies from excellent and top scientific journals
Cell. 2018 Jan 11;172(1-2):249-261.e12. doi: 10.1016/j.cell.2017.12.019.PMID: 29328914
Mol Cell. 2017 Nov 16;68(4):673-685.e6. doi: 10.1016/j.molcel.2017.10.022.PMID: 29149595
Scientific Reports 2017 Dec 11;7(1):17332.doi: 10.1038/s41598-017-17427-6.PMID: 29230013
Molecules. 2017 Oct 27;22(11). pii: E1829.doi: 10.3390/molecules22111829.PMID: 29077044
J Cell Biochem. 2018 Feb;119(2):2231-2239.doi: 10.1002/jcb.26385. PMID: 28857247
Phytomedicine. 2018 Feb 1;40:37-47. doi: 10.1016/j.phymed.2017.12.030.PMID: 29496173
Calculate Dilution Ratios(Only for Reference)
* Note: If you are in the process of experiment, it's need to make the dilution ratios of the samples. The dilution data of the sheet for your reference. Normally, it's can get a better solubility within lower of Concentrations.
|Plant Physiol. 2011 Mar;155(3):1127-45. |
|Overexpression of sinapine esterase BnSCE3 in oilseed rape seeds triggers global changes in seed metabolism.[Pubmed: 21248075]|
|Sinapine (O-sinapoylcholine) is the predominant phenolic compound in a complex group of sinapate esters in seeds of oilseed rape (Brassica napus). Sinapine has antinutritive activity and prevents the use of seed protein for food and feed. A strategy was developed to lower its content in seeds by expressing an enzyme that hydrolyzes Sinapine in developing rape seeds.
METHODS AND RESULTS:
During early stages of seedling development, a Sinapine esterase (BnSCE3) hydrolyzes Sinapine, releasing choline and sinapate. A portion of choline enters the phospholipid metabolism, and sinapate is routed via 1-O-sinapoyl-β-glucose into sinapoylmalate. Transgenic oilseed rape lines were generated expressing BnSCE3 under the control of a seed-specific promoter. Two distinct single-copy transgene insertion lines were isolated and propagated to generate homozygous lines, which were subjected to comprehensive phenotyping. Sinapine levels of transgenic seeds were less than 5% of wild-type levels, whereas choline levels were increased. Weight, size, and water content of transgenic seeds were significantly higher than those of wild-type seeds. Seed quality parameters, such as fiber and glucosinolate levels, and agronomically important traits, such as oil and protein contents, differed only slightly, except that amounts of hemicellulose and cellulose were about 30% higher in transgenic compared with wild-type seeds. Electron microscopic examination revealed that a fraction of the transgenic seeds had morphological alterations, characterized by large cavities near the embryonic tissue. Transgenic seedlings were larger than wild-type seedlings, and young seedlings exhibited longer hypocotyls. Examination of metabolic profiles of transgenic seeds indicated that besides suppression of Sinapine accumulation, there were other dramatic differences in primary and secondary metabolism.
Mapping of these changes onto metabolic pathways revealed global effects of the transgenic BnSCE3 expression on seed metabolism.
|Shi Yan Sheng Wu Xue Bao. 1993 Sep;26(3):269-74. |
|Radiation protection effects of sinapine on Drosophila melanogaster in a sex-linked recessive lethal test system[Pubmed: 8191802]|
|It has been shown that the radiation resistance of some cruciferous plants is related to some natural radiation protection substances in these plants. Sinapine, which has shown radiation protection effects on barley, wheat and mouse, is one of such substances distributed in cruciferous plants.
METHODS AND RESULTS:
In this paper, the radiation protection effects of Sinapine on Drosophila melanogaster in a sex-linked recessive lethal (SLRL) test system were observed. The Sinapine solution could be fed to the D. melanogaster (Oregon K). 40 Gy X-irradiation induced SLRL mutation rate of 8.96%. However, if 10 mg/ml Sinapine which was found to have no physiological toxicity or harmful effects on its reproductive function, was fed before 40 Gy X-irradiation, the SLRL mutation rate could be reduced to 0.40%, which was within the range of spontaneous SLRL mutation rate of Oregon K Drosophila, i.e. 0-0.4%. The potential of the using of the very strong radiation protection effects of Sinapine in anticarcinogenesis was also discussed.