Science | Nature | Cell | View More
Natural Products
Tenuifolin
ChemFaces products have been cited in many studies from excellent and top scientific journals
Product Name Tenuifolin
Price: $100 / 20mg
CAS No.: 20183-47-5
Catalog No.: CFN98157
Molecular Formula: C36H56O12
Molecular Weight: 680.37 g/mol
Purity: >=98%
Type of Compound: Triterpenoids
Physical Desc.: White powder
Source: The roots of Polygala tenuifolia Willd.
Solvent: DMSO, Pyridine, Methanol, Ethanol, etc.
Download: COA    MSDS    SDF    Manual
Similar structural: Comparison (Web)  (SDF)
Guestbook:
Contact Us
Order & Inquiry & Tech Support

Tel: (0086)-27-84237683
Tech: service@chemfaces.com
Order: manager@chemfaces.com
Address: 176, CheCheng Eest Rd., WETDZ, Wuhan, Hubei 430056, PRC
How to Order
Orders via your E-mail:

1. Product number / Name / CAS No.
2. Delivery address
3. Ordering/billing address
4. Contact information
Order: manager@chemfaces.com
Delivery time
Delivery & Payment method

1. Usually delivery time: Next day delivery by 9:00 a.m. Order now

2. We accept: Wire transfer & Credit card & Paypal
Citing Use of our Products
* Packaging according to customer requirements(5mg, 10mg, 20mg and more). We shipped via FedEx, DHL, UPS, EMS and others courier.
According to end customer requirements, ChemFaces provide solvent format. This solvent format of product intended use: Signaling Inhibitors, Biological activities or Pharmacological activities.
Size /Price /Stock 10 mM * 1 mL in DMSO / $30.8 / In-stock
Other Packaging *Packaging according to customer requirements(100uL/well, 200uL/well and more), and Container use Storage Tube With Screw Cap
Our products had been exported to the following research institutions and universities, And still growing.
  • University of Parma (Italy)
  • Biotech R&D Institute (USA)
  • National Research Council of Ca... (Canada)
  • The Ohio State University (USA)
  • University of Ioannina (Greece)
  • University of Lodz (Poland)
  • Aveiro University (Portugal)
  • Universidade Federal de Goias (... (Brazil)
  • University of Eastern Finland (Finland)
  • University of Brasilia (Brazil)
  • Medical University of South Car... (USA)
  • More...
Package
Featured Products
Astragaloside III

Catalog No: CFN99174
CAS No: 84687-42-3
Price: $268/20mg
Coptisine

Catalog No: CFN99563
CAS No: 3486-66-6
Price: $88/20mg
Procyanidin B2

Catalog No: CFN99558
CAS No: 29106-49-8
Price: $138/20mg
Cordycepin

Catalog No: CFN98566
CAS No: 73-03-0
Price: $40/20mg
Yatein

Catalog No: CFN98635
CAS No: 40456-50-6
Price: $ /
Abietic acid

Catalog No: CFN90587
CAS No: 514-10-3
Price: $40/20mg
alpha-Cyperone

Catalog No: CFN99703
CAS No: 473-08-5
Price: $100/20mg
Citropten

Catalog No: CFN97963
CAS No: 487-06-9
Price: $60/20mg
Ginsenoside Rg4

Catalog No: CFN90572
CAS No: 126223-28-7
Price: $ / mg
6-Hydroxyrubiadin

Catalog No: CFN97444
CAS No: 87686-86-0
Price: $218/5mg
Related Screening Libraries
Size /Price /Stock 10 mM * 100 uL in DMSO / Inquiry / In-stock
10 mM * 1 mL in DMSO / Inquiry / In-stock
Related Libraries
Biological Activity
Description: Tenuifolin is one of the active constituents of HPS against the neurotoxicity induced by Aβ25-35 peptides in vitro and in vivo, it possesses neuroprotective effects against Aβ25-35-induced apoptosis in PC12 cells and significantly improves the cognitive deficits induced by the intrahippocampal injection of Aβ25-35 in mice.
Targets: Beta Amyloid | AChR
In vitro:
Acta Physiol (Oxf). 2009 Aug;196(4):419-25.
Tenuifolin, an extract derived from tenuigenin, inhibits amyloid-beta secretion in vitro.[Pubmed: 19208093]
Previous studies have shown that tenuigenin, a crude extract of Polygala tenuifolia Willd. that is commonly used in traditional Chinese herbal medicine for memory loss, can reduce the secretion of Abeta from cultured cells. However, the mechanism underlying this effect and the active compound derived from tenuigenin is unknown. In this study, a purified component of tenuigenin, Tenuifolin, was examined and revealed to be an effective compound in vitro.
METHODS AND RESULTS:
Abeta secretion from three sets of COS-7 cells, each carrying a plasmid expressing a different form of APP was examined following the treatment with Tenuifolin. Initially, Tenuifolin was determined to have no inherent toxicity to either the transfected or wild type cells at the effective concentrations. Cells were then treated with 0.5-2.0 microg mL(-1) Tenuifolin for 12 h and their media were examined via an ELISA for Abeta1-40 and Abeta-42. We found that treatment with 2.0 microg mL(-1) Tenuifolin significantly decreased Abeta secretion from COS-7 cells without altering the ratio of Abeta1-40 and Abeta-42. This effect is most probably due to inhibition of the beta-site APP cleaving enzyme as Abeta secretion was not inhibited from cells expressing the C99 fragment.
CONCLUSIONS:
Tenuifolin is an effective compound from tenuigenin. We believe that this finding should lead the way for future experiments to determine the exact mechanism for Tenuifolin's effect on Abeta secretion.
Molecules 2012, 17(3), 3524-3538
Terpenoids as Potential Anti-Alzheimer’s Disease Therapeutics[Pubmed: 17033524]
Alzheimer’s disease (AD) is one of the most well-known neurodegenerative diseases and explains 50–60% of dementia in patients. The prevalence rate of AD is positively correlated with age and AD affects ≥ 40% of those over 85 years old. The major AD therapeutics available on the market are acetylcholinesterase inhibitors, such as tacrine and donepezil. New therapeutic agents that can block the disease-inducing mechanisms are essential. Diverse efforts have been made to discover anti-AD agents from natural sources. In this review article, we describe some representative terpenoids such as ginsenosides, gingkolides, and canabinoids as potential anti-AD agents. These compounds exhibit promising in vitro and in vivo biological activities, but are still waiting clinical trials. Additionally, we also discuss some terpenoids including cornel iridoid glycoside, oleanolic acid, Tenuifolin, cryptotanshinone, and ursolic acid, which are under investigation for their in vitro and in vivo animal studies.
In vivo:
Pharmacol Biochem Behav. 2015 Jan;128:14-22.
Tenuifolin, a secondary saponin from hydrolysates of polygalasaponins, counteracts the neurotoxicity induced by Aβ25-35 peptides in vitro and in vivo.[Pubmed: 25444865]
Alzheimer's disease (AD) is associated with damage to hippocampal neurons and declines in cognitive functions. The accumulation of amyloid peptides is regarded as a crucial event in the initiation of AD. The neurotoxicity induced by Aβ25-35 peptides was used to screen for cytoprotective factors in vitro, and the cognitive deficits induced by the injection of Aβ25-35 into the hippocampus were used to evaluate effect on learning and memory. Our previous study revealed that hydrolysate of polygalasaponins (HPS) clearly improve the cognitive deficits induced by the injection of Aβ25-35 in mice, but the potential active constituent of HPS remains unclear. The purposes of this study were to separate and purify the secondary saponins of HPS, screen for neuroprotective effects of the constituents in vitro, and to evaluate the effect of cognition in vivo.
METHODS AND RESULTS:
Various chromatographic methods were used to separate and purify the HPS. The neuroprotective effects were examined in Aβ25-35-damage-induced PC12 cells. The protective effect of Tenuifolin on the cognitive impairments induced by Aβ25-35 injection was assessed using the Morris water maze and step-through passive avoidance tests. Tenuifolin and fallaxsaponin A were isolated from the HPS. Tenuifolin possessed neuroprotective effects against Aβ25-35-induced apoptosis in PC12 cells and significantly improved the cognitive deficits induced by the intrahippocampal injection of Aβ25-35 in mice.
CONCLUSIONS:
Thus, Tenuifolin is one of the active constituents of HPS against the neurotoxicity induced by Aβ25-35 peptides in vitro and in vivo.
Tenuifolin Description
Source: The roots of Polygala tenuifolia Willd.
Solvent: DMSO, Pyridine, Methanol, Ethanol, etc.
Storage: Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).

Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

Need more advice on solubility, usage and handling? Please email to: service@chemfaces.com

After receiving: The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
ChemFaces New Products and Compounds
Lappaol C

Catalog No: CFN95068
CAS No: 64855-00-1
Price: $333/10mg
New compound 6

Catalog No: CFN95259
CAS No: N/A
Price: $413/5mg
Euphorbia factor L22

Catalog No: CFN95338
CAS No: 1613700-09-6
Price: $413/5mg
Isoeuphorbetin

Catalog No: CFN95207
CAS No: 50677-55-9
Price: $318/5mg
Rhamnocitrin 3-glucoside

Catalog No: CFN95134
CAS No: 41545-37-3
Price: $318/10mg
Carasinol D

Catalog No: CFN95044
CAS No: 1072797-66-0
Price: $333/5mg
Benzyl beta-D-glucopyranoside

Catalog No: CFN95427
CAS No: 4304-12-5
Price: $318/10mg
Isovalerylshikonin

Catalog No: CFN95222
CAS No: 52387-14-1
Price: $318/10mg
Recently, ChemFaces products have been cited in many studies from excellent and top scientific journals

Cell. 2018 Jan 11;172(1-2):249-261.e12.
doi: 10.1016/j.cell.2017.12.019.
IF=36.216(2019)

PMID: 29328914

Cell Metab. 2020 Mar 3;31(3):534-548.e5.
doi: 10.1016/j.cmet.2020.01.002.
IF=22.415(2019)

PMID: 32004475

Mol Cell. 2017 Nov 16;68(4):673-685.e6.
doi: 10.1016/j.molcel.2017.10.022.
IF=14.548(2019)

PMID: 29149595

ACS Nano. 2018 Apr 24;12(4): 3385-3396.
doi: 10.1021/acsnano.7b08969.
IF=13.903(2019)

PMID: 29553709

Nature Plants. 2016 Dec 22;3: 16206.
doi: 10.1038/nplants.2016.205.
IF=13.297(2019)

PMID: 28005066

Sci Adv. 2018 Oct 24;4(10): eaat6994.
doi: 10.1126/sciadv.aat6994.
IF=12.804(2019)

PMID: 30417089
Calculate Dilution Ratios(Only for Reference)
1 mg 5 mg 10 mg 20 mg 25 mg
1 mM 1.4698 mL 7.3489 mL 14.6979 mL 29.3958 mL 36.7447 mL
5 mM 0.294 mL 1.4698 mL 2.9396 mL 5.8792 mL 7.3489 mL
10 mM 0.147 mL 0.7349 mL 1.4698 mL 2.9396 mL 3.6745 mL
50 mM 0.0294 mL 0.147 mL 0.294 mL 0.5879 mL 0.7349 mL
100 mM 0.0147 mL 0.0735 mL 0.147 mL 0.294 mL 0.3674 mL
* Note: If you are in the process of experiment, it's need to make the dilution ratios of the samples. The dilution data of the sheet for your reference. Normally, it's can get a better solubility within lower of Concentrations.
Protocol
Animal Research:
Phytomedicine. 2008 Aug;15(8):587-94.
Effects of tenuifolin extracted from radix polygalae on learning and memory: a behavioral and biochemical study on aged and amnesic mice.[Pubmed: 18289838]
Although normal cognitive changes take place when a person becomes older, aging in humans is generally associated with deterioration of cognitive performance and, in particular, of learning and memory. These cognitive deficits can cause debilitating consequences due to aging. There are a number of herbal medicines which are reported to improve brain function including intelligence.
METHODS AND RESULTS:
In the present study, improving effects of Tenuifolin, extracted from Radix Polygalae (RP), on learning and memory in aged and dysmnesia mice were determined using step-down type passive avoidance test or Y type maze trial. Oral administration of Tenuifolin (0.02, 0.04, 0.08 g/kgd(-1), for 15 d) evidently improved the latency and number of errors in aged and dysmnesia mice. The levels of cortical acetylcholine esterase (AChE) activity and hippocampal neurotransmitters in aged mice given Tenuifolin (0.02, 0.04, 0.08 g/kgd(-1), for 15 d) were also investigated, and increased levels of norepinephrine (NE), dopamine (DA), decreased activity of AChE were found. However, serotonin (5-HT) had no significant difference from that of aged mice given distilled water.
CONCLUSIONS:
The evident improvement of learning and memory of aged mice is carried out by the effects of Tenuifolin on the three stages of memory process, that is, acquisition, consolidation and retrieval. This may do so by relatively increasing the levels of NE, DA in the hippocampus and by decreasing the activity of AChE in the cortex.
Astragaloside III

Catalog No: CFN99174
CAS No: 84687-42-3
Price: $268/20mg
Coptisine

Catalog No: CFN99563
CAS No: 3486-66-6
Price: $88/20mg
Procyanidin B2

Catalog No: CFN99558
CAS No: 29106-49-8
Price: $138/20mg
Cordycepin

Catalog No: CFN98566
CAS No: 73-03-0
Price: $40/20mg
Yatein

Catalog No: CFN98635
CAS No: 40456-50-6
Price: $ /
Abietic acid

Catalog No: CFN90587
CAS No: 514-10-3
Price: $40/20mg
alpha-Cyperone

Catalog No: CFN99703
CAS No: 473-08-5
Price: $100/20mg
Citropten

Catalog No: CFN97963
CAS No: 487-06-9
Price: $60/20mg
Ginsenoside Rg4

Catalog No: CFN90572
CAS No: 126223-28-7
Price: $ / mg
6-Hydroxyrubiadin

Catalog No: CFN97444
CAS No: 87686-86-0
Price: $218/5mg
Tags: buy Tenuifolin | Tenuifolin supplier | purchase Tenuifolin | Tenuifolin cost | Tenuifolin manufacturer | order Tenuifolin | Tenuifolin distributor