|Description:||1. Tenuifoliside A has anti-apoptotic activity.|
2. Tenuifoliside A has neuroprotective activity.
3. Tenuifoliside A has anti-inflammatory effect, is mediated by the inhibition of the NF-κB and MAPK pathways.
|Targets:||NO | NOS | PGE | COX | TNF-α | p65 | NF-kB | IkB | MAPK | JNK | ERK | PI3K | Akt | IKK|
|Source:||The roots of Polygala tenuifolia.|
|Solvent:||DMSO, Pyridine, Methanol, Ethanol, etc.|
|Storage:||Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please email to: firstname.lastname@example.org
|After receiving:||The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.|
|1 mg||5 mg||10 mg||20 mg||25 mg|
|1 mM||1.465 mL||7.3249 mL||14.6499 mL||29.2997 mL||36.6247 mL|
|5 mM||0.293 mL||1.465 mL||2.93 mL||5.8599 mL||7.3249 mL|
|10 mM||0.1465 mL||0.7325 mL||1.465 mL||2.93 mL||3.6625 mL|
|50 mM||0.0293 mL||0.1465 mL||0.293 mL||0.586 mL||0.7325 mL|
|100 mM||0.0146 mL||0.0732 mL||0.1465 mL||0.293 mL||0.3662 mL|
Eur J Pharmacol. 2013 Dec 5;721(1-3):267-76.
|The inhibition of JNK MAPK and NF-κB signaling by tenuifoliside A isolated from Polygala tenuifolia in lipopolysaccharide-induced macrophages is associated with its anti-inflammatory effect.[Pubmed: 24076326]|
|The root of Polygala tenuifolia Willd. (Polygalaceae) is well known for its use in the treatment of neurasthenia, amnesia, and inflammation. In this study, we isolated phenyl propanoid type metabolite Tenuifoliside A, one of the phenylpropanoids from P. tenuifolia, and investigated its anti-inflammatory effects in lipopolysaccharide (LPS)-stimulated RAW264.7 and murine peritoneal macrophages. The results showed that Tenuifoliside A inhibited the production of nitric oxide (NO), inducible nitric oxide synthase (iNOS), prostaglandin E2 (PG E2), and cyclooxygenase (COX)-2. In addition, Tenuifoliside A suppressed the production of pro-inflammatory cytokines, such as tumor necrosis factor (TNF)-α and interleukin (IL)-1β. We also evaluated the effects of Tenuifoliside A on the activation of nuclear factor-kappaB (NF-κB). Tenuifoliside A inhibited the translocation of the NF-κB subunit p65 into the nucleus by interrupting the phosphorylation and degradation of inhibitor kappa B (IκB)-α in LPS-stimulated murine peritoneal macrophages. Moreover, we confirmed that the suppression of the inflammatory process by Tenuifoliside A was mediated through the mitogen-activated protein kinases (MAPKs) pathway based on the fact that Tenuifoliside A significantly decreased p-c-Jun N-terminal kinase (p-JNK) protein expression in LPS-stimulated murine peritoneal macrophages. Taken together, the anti-inflammatory effects of Tenuifoliside A were mediated by the inhibition of the NF-κB and MAPK pathways. This study is the first report on the anti-inflammatory effects of Tenuifoliside A, and the strong anti-inflammatory effects of Tenuifoliside A provide potential compound to be developed as therapeutic for inflammatory diseases.|
Phytomedicine. 2014 Sep 15;21(10):1178-88.
|Effect of Tenuifoliside A isolated from Polygala tenuifolia on the ERK and PI3K pathways in C6 glioma cells.[Pubmed: 24877714]|
|Tenuifoliside A (TFSA) is a bioactive oligosaccharide ester component of Polygala tenuifolia Wild, a traditional Chinese medicine which was used to manage mental disorders effectively. The neuroprotective and anti-apoptotic effects of Tenuifoliside A have been demonstrated in our previous studies. The present work was designed to study the molecular mechanism of Tenuifoliside A on promoting the viability of rat glioma cells C6. We exposed C6 cells to Tenuifoliside A (or combined with ERK, PI3K and TrkB inhibitors) to examine the effects of Tenuifoliside A on the cell viability and the expression and phosphorylation of key proteins in the ERK and PI3K signaling pathway. Tenuifoliside A increased levels of phospho-ERK and phospho-Akt, enhanced release of BDNF, which were blocked by ERK and PI3K inhibitors, respectively (U0126 and LY294002). Moreover, the Tenuifoliside A caused the enhanced phosphorylation of cyclic AMP response element binding protein (CREB) at Ser133 site, the effect was revoked by U0126, LY294002 and K252a. Furthermore, when C6 cells were pretreated with K252a, a TrkB antagonist, known to significantly inhibit the activity of brain-derived neurotrophic factor (BDNF), blocked the levels of phospho-ERK, phospho-Akt and phosphor-CREB. Taking these results together, we suggested the neuroprotection of Tenuifoliside A might be mediated through BDNF/TrkB-ERK/PI3K-CREB signaling pathway in C6 glioma cells.|
Rev Neurosci. 2015;26(3):305-21.
|Plant-derived natural medicines for the management of depression: an overview of mechanisms of action.[Pubmed: 25719303]|
|The total glycosides of Paeonia lactiflora demonstrate an inhibitory effect on both subtypes of monoamine oxidase. 3,6'-Di-o-sinapoyl-sucrose and Tenuifoliside A from Polygala tenuifolia exhibit cytoprotective effects on neuronal cells. Further preclinical and clinical trials evaluating their safety, bioefficacy, and bioavailability are suggested to prove the valuable role of natural drugs in the management of depressive disorders.|
Phytomedicine. 2010 Aug;17(10):794-9.
|Potential antidepressant properties of Radix Polygalae (Yuan Zhi).[Pubmed: 20541923]|
|It was found that YZ-50 and its two bioactive compounds, 3,6'-di-o-sinapoyl-sucrose (DISS) and Tenuifoliside A(TEA) showed protection activities in SY5Y cells from the lesion. By using bioassay-screening methods, our results indicate that the presence of oligosaccharide esters such as DISS and Tenuifoliside A in this herb may be responsible for the cytoprotective activity effects.|