Info: Read More
  • Home
  • Natural Products
  • Bioactive
  • Screening Libraries
  • Hot Products
  • Plant Catalog
  • Customer Support
  • Product Use Citation
  • About Us
  • Contact Us
  • How to Order
  • Delivery time
  • Science | Nature | Cell | View More
    Natural Products
    Bergapten
    Information
    CAS No. 484-20-8 Price $40 / 20mg
    Catalog No.CFN98766Purity>=98%
    Molecular Weight216.2 Type of CompoundCoumarins
    FormulaC12H8O4Physical DescriptionPowder
    Download Manual    COA    MSDS    SDFSimilar structuralComparison (Web)  (SDF)
    Citing Use of our Products
    How to Order
    Orders via your E-mail:

    1. Product number / Name / CAS No.
    2. Delivery address
    3. Ordering/billing address
    4. Contact information
    Sent to Email: info@chemfaces.com
    Contact Us
    Order & Inquiry & Tech Support

    Tel: (0086)-27-84237683
    Fax: (0086)-27-84254680
    E-mail: manager@chemfaces.com
    Address: No. 83, CheCheng Rd., WETDZ, Wuhan, Hubei 430056, PRC
    Delivery time
    Delivery & Payment method

    1. Usually delivery time: Next day delivery by 9:00 a.m. Order now

    2. We accept: Wire transfer & Credit card & Paypal & Western Union
    * Packaging according to customer requirements(5mg, 10mg, 20mg and more). We shipped via FedEx, DHL, UPS, EMS and others courier.
    According to end customer requirements, ChemFaces provide solvent format. This solvent format of product intended use: Signaling Inhibitors, Biological activities or Pharmacological activities.
    Size /Price /Stock 10 mM * 1 mL in DMSO / $11.8 / In-stock
    Other Packaging *Packaging according to customer requirements(100uL/well, 200uL/well and more), and Container use Storage Tube With Screw Cap
    Our products had been exported to the following research institutions and universities, And still growing.
  • Julius Kühn-Institut (Germany)
  • Medical University of Gdansk (Poland)
  • Michigan State University (USA)
  • Universitas islam negeri Jakarta (Indonesia)
  • University of Bordeaux (France)
  • Mahidol University (Thailand)
  • University of Lodz (Poland)
  • Universiti Sains Malaysia (Malaysia)
  • University of Wuerzburg (Germany)
  • Georgia Institute of Technology (USA)
  • Universidade do Porto (Portugal)
  • More...
  • Package
    Featured Products
    Desmethoxy yangonin

    Catalog No: CFN90149
    CAS No: 15345-89-8
    Price: $188/20mg
    Astragalin

    Catalog No: CFN98733
    CAS No: 480-10-4
    Price: $138/20mg
    Leachianone A

    Catalog No: CFN97560
    CAS No: 97938-31-3
    Price: $218/5mg
    Chlorogenic acid

    Catalog No: CFN99116
    CAS No: 327-97-9
    Price: $30/20mg
    Sibiricaxanthone B

    Catalog No: CFN90644
    CAS No: 241125-81-5
    Price: $238/20mg
    Glycitein

    Catalog No: CFN99106
    CAS No: 40957-83-3
    Price: $128/20mg
    8-Prenylnaringenin

    Catalog No: CFN92016
    CAS No: 53846-50-7
    Price: $138/10mg

    Bergapten

    Bergapten
    Product Name Bergapten
    CAS No.: 484-20-8
    Catalog No.: CFN98766
    Molecular Formula: C12H8O4
    Molecular Weight: 216.2 g/mol
    Purity: >=98%
    Type of Compound: Coumarins
    Physical Desc.: Powder
    Targets: TGF-尾/Smad | Caspase | TNF-伪 | IL Receptor | PI3K | p53 | Akt | p21
    Source: The roots of Angelica dahurica.
    Solvent: Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.
    Price: $40 / 20mg
    Inquire / Order: manager@chemfaces.com
    Technical Inquiries: service@chemfaces.com
    Tel: +86-27-84237783
    Fax: +86-27-84254680

    Address:
    1 Building, No. 83, CheCheng Rd., Wuhan Economic and Technological Development Zone, Wuhan, Hubei 430056, PRC
  • Pathogens.2018, 7(3):E62
  • Ulm University Medical Center2020, doi: 10.18725.
  • J. of Med. Plant Research.2013, 90-151
  • J Chromatogr B Analyt Technol Biomed Life Sci. 2017, 1064:115-123
  • J of the Korean Society of Cosmetics and Cosmetology2019, 225-231
  • J Chromatogr B Analyt Technol Biomed Life Sci.2019, 1124:323-330
  • US20170000760 A12016, 42740
  • Korean J. Medicinal Crop Sci.2018, 26(2):148-156
  • BMC Complement Med Ther. 2020, 20(1):91.
  • The Journal of Animal & Plant Sciences.2020, 30(6):1366-1373
  • Related Screening Libraries
    Size /Price /Stock 10 mM * 100 uL in DMSO / Inquiry / In-stock
    10 mM * 1 mL in DMSO / Inquiry / In-stock
    Related Libraries
  • Anticancer Compound Library
  • Anti-inflammatory Compound Library
  • Anti-photoaging Compound Library
  • Anti-bone loss Compound Library
  • Coumarins Compound Library
  • TNF-α Inhibitor Library
  • TGF-β/Smad Inhibitor Library
  • PI3K Inhibitor Library
  • p53 Inhibitor Library
  • p21 Inhibitor Library
  • IL Receptor Inhibitor Library
  • Caspase Inhibitor Library
  • Akt Inhibitor Library
  • Biological Activity
    Description: Bergapten is a psoralen that can be photoactivated and is capable of crossing-linking DNA, covalently modifying proteins and lipids, and consequently inhibiting cell replication. Bergapten has anti-inflammatory and anti-tumor agent, it exhibits significant inhibition of the production of pro-inflammatory cytokines, namely tumour necrotic factor-α(TNF-α) and interleukin-6 (IL-6) by peripheral blood mononuclear cells (PBMCs) stimulated with lipopolysaccharide in a concentration-dependent manner. Bergapten effectively prevents LPS-induced osteoclastogenesis, bone resorption and survival via apoptotic response of osteoclasts and their precursors.
    Targets: TGF-β/Smad | Caspase | TNF-α | IL Receptor | PI3K | p53 | Akt | p21
    In vitro:
    Int Orthop. 2014 Mar;38(3):627-34.
    Bergapten prevents lipopolysaccharide mediated osteoclast formation, bone resorption and osteoclast survival.[Pubmed: 24305787]

    METHODS AND RESULTS:
    To investigate the effect of Bergapten on osteoclastic bone resorption, RAW264.7 cells were treated with Bergapten for six days in the presence of LPS, and the area of bone resorption was analyzed with Image Pro-Plus. Next, we examined apoptosis of RAW264.7 cells after Bergapten incubation for 48 hours by flow cytometer using annexin V/propidium iodide (PI) double labeling. Finally, osteoclast survival was observed by Hoechst 33342 labeling and Western blotting after Bergapten treatment for 24 hours. RESULTS: Data showed that Bergapten (5-40 μmol/L) dose-dependently inhibited LPS-induced osteoclast formation and bone resorption. Treatment with Bergapten triggered apoptotic death of osteoclast precursor RAW264.7 cells in a dose-dependent manner. Furthermore, Bergapten significantly reduced the survival of mature osteoclast, as demonstrated by emergence of apoptotic nuclei and activation of apoptotic protein caspase 3/9.
    CONCLUSIONS:
    These findings suggest that Bergapten effectively prevents LPS-induced osteoclastogenesis, bone resorption and survival via apoptotic response of osteoclasts and their precursors. The study identifies Bergapten as an inhibitor of osteoclast formation and bone resorption and provides evidence that Bergapten might be beneficial as an alternative for prevention and treatment of inflammatory bone loss.
    Nat Prod Res. 2011 Sep;25(15):1444-9.
    Effect of bergapten from Heracleum nepalense root on production of proinflammatory cytokines.[Pubmed: 19662568]
    In the present investigation, the anti-inflammatory activity of isolated Bergapten from hydroalcoholic extract of Heracleum nepalense root was evaluated in vitro using human peripheral blood mononuclear cells (PBMCs).
    METHODS AND RESULTS:
    Bergapten exhibited significant inhibition of the production of pro-inflammatory cytokines, namely tumour necrotic factor-α (TNF-α) and interleukin-6 (IL-6) by PBMCs stimulated with lipopolysaccharide in a concentration-dependent manner.
    In vivo:
    J Am Acad Dermatol. 1988 Feb;18(2 Pt 1):333-8.
    5-Methoxypsoralen (Bergapten) for photochemotherapy. Bioavailability, phototoxicity, and clinical efficacy in psoriasis of a new drug preparation.[Pubmed: 3279089]
    In a previous study we evaluated a microcrystalline preparation of 5-methoxypsoralen (5-MOP; Bergapten) for its photochemotherapeutic properties. Preliminary data indicated that the clinical efficacy of 5-MOP is comparable to that of 8-methoxypsoralen. 5-MOP appeared as a promising alternative photosensitizer for the management of psoriasis because of the almost complete lack of phototoxic and drug intolerance reactions that are frequently encountered in patients undergoing 8-MOP photochemotherapy.
    METHODS AND RESULTS:
    With a new liquid preparation of 5-MOP we have now extended our earlier investigation on a larger clinical scale and have correlated the clinical response with the bioavailability of the drug. Serum level determinations showed an absorption rate of only approximately 25% that of 8-MOP. When administered in the same dosage as 8-MOP, 5-MOP turned out to be significantly less effective; however, by doubling the oral dosage, comparable results in terms of clearing of psoriasis were obtained. Also with this high-dose 5-MOP regimen, no drug intolerance was noted and other side effects, such as severe erythema, pruritus, and nausea, occurred only rarely.
    CONCLUSIONS:
    We propose 5-MOP as a valuable alternative for photochemotherapy (PUVA) of PUVA-responsive diseases.
    Bergapten Description
    Source: The roots of Angelica dahurica.
    Solvent: Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.
    Storage: Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Need more advice on solubility, usage and handling? Please email to: service@chemfaces.com

    After receiving: The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
    ChemFaces New Products and Compounds
    3,3',4',5,6,7,8-heptamethoxyflavon...

    Catalog No: CFN95021
    CAS No: 1178-24-1
    Price: $268/10mg
    Epischisandrone

    Catalog No: CFN95212
    CAS No: 98619-26-2
    Price: $413/5mg
    Bruceoside A

    Catalog No: CFN95080
    CAS No: 63306-30-9
    Price: $318/10mg
    Lappaol B

    Catalog No: CFN95242
    CAS No: 62359-60-8
    Price: $333/10mg
    Baohuoside II

    Catalog No: CFN90764
    CAS No: 55395-07-8
    Price: $268/10mg
    Isoliquiritin

    Catalog No: CFN99155
    CAS No: 5041-81-6
    Price: $138/20mg
    6',7'-Dihydroxybergamottin

    Catalog No: CFN98307
    CAS No: 264234-05-1
    Price: $438/10mg
    (-)-Gallocatechin

    Catalog No: CFN99137
    CAS No: 3371-27-5
    Price: $178/20mg
    Recently, ChemFaces products have been cited in many studies from excellent and top scientific journals

    Cell. 2018 Jan 11;172(1-2):249-261.e12.
    doi: 10.1016/j.cell.2017.12.019.
    IF=36.216(2019)

    PMID: 29328914

    Cell Metab. 2020 Mar 3;31(3):534-548.e5.
    doi: 10.1016/j.cmet.2020.01.002.
    IF=22.415(2019)

    PMID: 32004475

    Mol Cell. 2017 Nov 16;68(4):673-685.e6.
    doi: 10.1016/j.molcel.2017.10.022.
    IF=14.548(2019)

    PMID: 29149595

    ACS Nano. 2018 Apr 24;12(4): 3385-3396.
    doi: 10.1021/acsnano.7b08969.
    IF=13.903(2019)

    PMID: 29553709

    Nature Plants. 2016 Dec 22;3: 16206.
    doi: 10.1038/nplants.2016.205.
    IF=13.297(2019)

    PMID: 28005066

    Sci Adv. 2018 Oct 24;4(10): eaat6994.
    doi: 10.1126/sciadv.aat6994.
    IF=12.804(2019)

    PMID: 30417089
    Calculate Dilution Ratios(Only for Reference)
    1 mg 5 mg 10 mg 20 mg 25 mg
    1 mM 4.6253 mL 23.1267 mL 46.2535 mL 92.5069 mL 115.6337 mL
    5 mM 0.9251 mL 4.6253 mL 9.2507 mL 18.5014 mL 23.1267 mL
    10 mM 0.4625 mL 2.3127 mL 4.6253 mL 9.2507 mL 11.5634 mL
    50 mM 0.0925 mL 0.4625 mL 0.9251 mL 1.8501 mL 2.3127 mL
    100 mM 0.0463 mL 0.2313 mL 0.4625 mL 0.9251 mL 1.1563 mL
    * Note: If you are in the process of experiment, it's need to make the dilution ratios of the samples. The dilution data of the sheet for your reference. Normally, it's can get a better solubility within lower of Concentrations.
    Protocol
    Kinase Assay:
    Breast Cancer Res Treat. 2012 Nov;136(2):443-55.
    Bergapten induces ER depletion in breast cancer cells through SMAD4-mediated ubiquitination.[Pubmed: 23053665]
    ERα function is crucial for the development of normal mammary gland as well as in the process of progression of breast cancer cells. Signals that target receptor levels contribute to regulate estrogens effects in the cells. An intricate cross-regulation has been documented between ERα and TGF-β down-stream molecules: SMAD2, SMAD3, and SMAD4, that can bind ERα and regulate their signaling. Thus, identification of natural anticancer drugs able to influence the latter molecule might provide alternative choices for breast cancer treatment. Taking into account our previous published data we wanted to study the effect of 5-Methoxypsoralen (Bergapten) on ERα and on TGF-β pathway.
    METHODS AND RESULTS:
    We reported that Bergapten, a coumarin containing compound, effectively depletes ERα in MCF-7 breast cancer sensitive cells and in tamoxifen-resistant clone. The decrease of ERα protein after Bergapten treatment results from the ubiquitine-proteasome pathway as demonstrated by the use of MG-132. IP experiments with ER antibody, demonstrated that the protein has physical interaction with SMAD4 and poly-ubiquitine and the amount of ubiquitinated receptor, linked to SMAD4, is greater under Bergapten. The crucial role played by SMAD4, in this process, emerges from the observation that in breast cancer cells, silencing of SMAD4, resulted in increased expression of endogenous ERα in both control and Bergapten-treated cells, compared to wild- type cells. The same results were confirmed in siRNA TGF-β RII cells.
    The results suggest a novel negative regulation of ERα by TGF-β/SMAD4 in breast cancer cells and indicate that the SMAD4 protein is involved in the degradation of ERα induced by Bergapten. CONCLUSIONS:
    We propose that Bergapten may efficiently act as a natural antitumoral agent, able to deplete ERα from breast cancer tamoxifen-sensitive and resistant cells, thereby retraining the effect of membrane signals targeting ERα and in such way its mitogenic potentiality.
    FEBS Lett. 2010 Jun 3;584(11):2321-6.
    Breast cancer cell survival signal is affected by bergapten combined with an ultraviolet irradiation.[Pubmed: 20371365]

    METHODS AND RESULTS:
    In this study we have reported that Bergapten (B) and Bergapten plus UV (PUVA) are able to significantly affect MCF-7, ZR-75 and SKBR-3 breast cancer cell proliferations. B induced a lowering of PI3K/AKT survival signal in MCF-7 cells even in presence of IGF-I stimulation. Furthermore, B and in a higher extent, PUVA up-regulated the p53 mRNA and the protein content. An increased co-association between p21 WAF and proliferating cell nuclear antigen (PCNA) has been observed in PUVA-treated MCF-7 cells, thus inhibiting DNA replication.
    CONCLUSIONS:
    These results highlight how B, and its photoactivated compound, exert antiproliferative effects and induce apoptotic responses in breast cancer cells.
    Desmethoxy yangonin

    Catalog No: CFN90149
    CAS No: 15345-89-8
    Price: $188/20mg
    Astragalin

    Catalog No: CFN98733
    CAS No: 480-10-4
    Price: $138/20mg
    Leachianone A

    Catalog No: CFN97560
    CAS No: 97938-31-3
    Price: $218/5mg
    Chlorogenic acid

    Catalog No: CFN99116
    CAS No: 327-97-9
    Price: $30/20mg
    Sibiricaxanthone B

    Catalog No: CFN90644
    CAS No: 241125-81-5
    Price: $238/20mg
    Glycitein

    Catalog No: CFN99106
    CAS No: 40957-83-3
    Price: $128/20mg
    8-Prenylnaringenin

    Catalog No: CFN92016
    CAS No: 53846-50-7
    Price: $138/10mg