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| Size /Price /Stock |
10 mM * 1 mL in DMSO / Inquiry |
Other Packaging |
*Packaging according to customer requirements(100uL/well, 200uL/well and more), and Container use Storage Tube With Screw Cap |
More articles cited ChemFaces products.
Antiviral Res.2013, 98(3):386-93Fitoterapia.2015, 100:179-86Vojnosanit Pregl2016, 75(00):391-391Food and Bioprocess Technology...2017...Int J Anal Chem.2017, 2017:1254721Pharm Biol.2017, 55(1):360-366Nutrients.2018, 10(10)Front Aging Neurosci.2018, 10:269
Food Res Int.2018, 106:909-919BMC Complement Altern Med....2018...Int J Mol Sci.2018, 19(9):E2528Biofactors.2018, 44(2):168-179J of the Society of Cosmetic Scie...2018...J Nat Prod.2018, 81(4):966-975J Chromatogr B Analyt Technol Bio...2019...J Nat Prod.2019, 82(4):1002-1008
Biomolecules.2020, 10(2):E184South African J of Botany...2020...Biochem Pharmacol. 2020, 177:114014.Molecules.2020, 25(23):5636.Molecules.2020, 25(7):1625.J of Applied Biological Chem....2020...Clin Exp Pharmacol Physiol....2020...
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Our products had been exported to the following research institutions and universities, And still growing.
Sapienza University of Rome (Italy)John Innes Centre (United Kingdom)Chinese University of Hong Kong (China)Semmelweis Unicersity (Hungary)
University of Bordeaux (France)Uniwersytet Jagielloński w Kra... (Poland)Auburn University (USA)Guangzhou Institutes of Biomedi... (China)
Universidad de Ciencias y Artes... (Mexico)University of Eastern Finland (Finland)University of East Anglia (United Kingdom)
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Hydroxytanshinone IIA
Inquire / Order:
manager@chemfaces.com
Technical Inquiries:
service@chemfaces.com
Tel:
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Fax:
+86-27-84254680
Address:
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Acta Edulis Fungi2020, 27(02):63-76.Int J Biol Macromol.2020, 161:1230-1239.Metabolites.2020, 11(1):E11.Evid Based Complement Alternat Med.2018, 2018:4580627Anat Rec2018, 24264Phytother Res.2019, 10.1002Food Res Int.2017, 96:40-45Nutraceutical Research . 2021, 19(1),p90-105.Korean J of Medicinal Crop Science2018, 220-226Biol Pharm Bull.2017, 40(6):797-806
Related Screening Libraries
| Description: |
Hydroxytanshinone ⅡA has good antiproliferative effect on SGC-7901,HeLa, and HepG 2 cell, the values of IC50 are 4.18, 6.08 and10.20 uM, respectively; it has tumor cell proliferation inhibition significantly stronger than the tanshinoneⅡA. |
| In vitro: |
| Rapid Commun Mass Spectrom. 2006;20(5):815-22. | | Simultaneous determination of tanshinone IIA and its three hydroxylated metabolites by liquid chromatography/tandem mass spectrometry.[Pubmed: 16470728] |
METHODS AND RESULTS:
A rapid and sensitive method based on liquid chromatography/tandem mass spectrometry (LC/MS/MS) for the simultaneous determination of tanshinone IIA and its three hydroxylated metabolites, tanshinone IIB, Hydroxytanshinone IIA and przewaquinone A, in a rat liver microsome was developed and fully validated. A single step of liquid-liquid extraction with ethyl acetate was utilized in this method. Chromatographic separation of the sample matrix from the analytes and the internal standard diazepam was performed using a Shim-pack VP-ODS analytical column. Detection was performed on a triple quadrupole tandem mass spectrometer equipped with an electrospray ionization source and operated in selected reaction monitoring (SRM) mode. The method was linear in the concentration range of 1-500 ng/mL for all analytes. The intra- and inter-day precisions (RSD %) were within 15% and deviations of the assay accuracies were within 15.0% for all analytes. The analytes proved to be stable during sample storage, preparation and analyses. This validated method was successfully applied to the enzyme kinetic study of tanshinone IIA in liver microsome.
CONCLUSIONS:
The elimination of tanshinone IIA and formation of tanshinone IIB and Hydroxytanshinone IIA in the liver microsome all exhibited a sigmoidal kinetics profile. The formation of przewaquinone A shows a typical hyperbolic profile. In addition, this method has now been applied in the analysis of other bio-samples including plasma, urine, bile and feces. |
|
| In vivo: |
| Journal of Chromatography A Volume 1104, Issues 1–2, 3 February 2006, Pages 366–369 | | Identification of tanshinone IIA metabolites in rat liver microsomes by liquid chromatography–tandem mass spectrometry[Reference: WebLink] | Tanshinone IIA, the major component extracted from Radix salvia miltiorrhiza, has been observed to possess various kinds of pharmacological activities including antioxidant, prevention of angina pectoris and myocardial infarction and anticancer.
METHODS AND RESULTS:
Tanshinone IIA was incubated with rat liver microsomes and the resulting metabolites were identified by liquid chromatography/tandem mass spectrometry. The results showed the formation of three main hydroxyl metabolites. The three hydroxyl metabolites of tanshinone IIA were proved to be tanshinone IIB, Hydroxytanshinone IIA and przewaquinone A by comparing the tandem mass spectra and the chromatographic retention time with that of the respective authentic compounds.
CONCLUSIONS:
Tanshinone IIB, Hydroxytanshinone IIA and przewaquinone A are all the chemical components of total tanshinones. It was reasonable to presume that the three hydroxy metabolites of tanshinone IIA were pharmacologically active the same as tanshinone IIA and the total tanshinones. | | J Mass Spectrom. 2006 May;41(5):670-84. | | Characterization of metabolites of tanshinone IIA in rats by liquid chromatography/tandem mass spectrometry.[Pubmed: 16598708] | The metabolism of tanshinone IIA was studied in rats after a single-dose intravenous administration.
METHODS AND RESULTS:
In the present study, 12 metabolites of tanshinone IIA were identified in rat bile, urine and feces with two LC gradients using LC-MS/MS. Seven phase I metabolites and five phase II metabolites of tanshinone IIA were characterized and their molecular structures proposed on the basis of the characteristics of their precursor ions, product ions and chromatographic retention time. The seven phase I metabolites were formed, through two main metabolic routes, which were hydroxylation and dehydrogenation metabolism. M1, M4, M5 and M6 were supposedly tanshinone IIB, Hydroxytanshinone IIA, przewaquinone A and dehydrotanshinone IIA, respectively, by comparing their HPLC retention times and mass spectral patterns with those of the standard compounds. The five phase II metabolites identified in this research were all glucuronide conjugates, all of which showed a neutral loss of 176 Da. M9 and M12 were more abundant than other identified metabolites in the bile, which was the main excretion path of tanshinone IIA and the metabolites.
CONCLUSIONS:
M12 was the main metabolite of tanshinone IIA. M9 and M12 were proposed to be the glucuronide conjugates of two different semiquinones and these semiquinones were the hydrogenation products of dehydrotanshinone IIA and tanshinone IIA, respectively.
This hydrogenized reaction may be catalyzed by the NAD(P)H: quinone acceptor oxidoreductase (NQO). The biotransformation pathways of tanshinone IIA were proposed on the basis of this research. |
|
Hydroxytanshinone IIA Description
| Source: |
The roots of Salvia miltiorrhiza Bge. |
| Solvent: |
Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc. |
| Storage: |
Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please email to: service@chemfaces.com
|
| After receiving: |
The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling. |
ChemFaces New Products and Compounds
Recently, ChemFaces products have been cited in many studies from excellent and top scientific journals
Cell. 2018 Jan 11;172(1-2):249-261.e12. doi: 10.1016/j.cell.2017.12.019.
IF=36.216(2019)PMID: 29328914
Cell Metab. 2020 Mar 3;31(3):534-548.e5. doi: 10.1016/j.cmet.2020.01.002.
IF=22.415(2019)PMID: 32004475
Mol Cell. 2017 Nov 16;68(4):673-685.e6. doi: 10.1016/j.molcel.2017.10.022.
IF=14.548(2019)PMID: 29149595
ACS Nano. 2018 Apr 24;12(4): 3385-3396. doi: 10.1021/acsnano.7b08969.
IF=13.903(2019)PMID: 29553709
Nature Plants. 2016 Dec 22;3: 16206. doi: 10.1038/nplants.2016.205.
IF=13.297(2019)PMID: 28005066
Sci Adv. 2018 Oct 24;4(10): eaat6994. doi: 10.1126/sciadv.aat6994.
IF=12.804(2019)PMID: 30417089
Calculate Dilution Ratios(Only for Reference)
|
1 mg |
5 mg |
10 mg |
20 mg |
25 mg |
| 1 mM |
3.2222 mL |
16.1108 mL |
32.2217 mL |
64.4434 mL |
80.5542 mL |
| 5 mM |
0.6444 mL |
3.2222 mL |
6.4443 mL |
12.8887 mL |
16.1108 mL |
| 10 mM |
0.3222 mL |
1.6111 mL |
3.2222 mL |
6.4443 mL |
8.0554 mL |
| 50 mM |
0.0644 mL |
0.3222 mL |
0.6444 mL |
1.2889 mL |
1.6111 mL |
| 100 mM |
0.0322 mL |
0.1611 mL |
0.3222 mL |
0.6444 mL |
0.8055 mL |
* Note: If you are in the process of experiment, it's need to make the dilution ratios of the samples. The dilution data of the sheet for your reference. Normally, it's can get a better solubility within lower of Concentrations.
| Cell Research: |
| Natural products research and development, 2015 (12): 2027-30. | | Synthesis and in vitro Anti-Tumor Effects of Hydroxytanshinone ⅡA.[Reference: WebLink] | Hydroxytanshinone IIA was synthesized with tanshinone ⅡA as lead compound.
METHODS AND RESULTS:
MTT assay was adopted to evaluate its inhibition effect against Hela,HepG-2 and SGC-7901 cells.Results indicated that Hydroxytanshinone IIA inhibited cell proliferation in a dose-dependent manner.The half inhibitory concentrations(IC_(50)) of Hydroxytanshinone IIA against SGC-7901,HeLa and HepG-2 cells were 4.18 μM,6.08 μM and 10.20 μM,respectively.But the IC_(50) of tanshinone ⅡA against SGC-7901,HeLa and HepG-2 cells were 17.15 μM 27.28 μM and 46.34 μM,respectively.
CONCLUSIONS:
The inhibition effect of Hydroxytanshinone IIA to SGC-7901 cells was powerful.Compared with tanshinone ⅡA,Hydroxytanshinone IIA significantly increased inhibition effect(P 0.05). |
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