|Source:||The peel of Punica granatum L.|
|Biological Activity or Inhibitors:|| 1. 1,3,6-Tri-O-galloylglucose shows anti-inflammatory activity.
|Solvent:||DMSO, Pyridine, Methanol, Ethanol, etc.|
|Storage:||Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please email to: email@example.com
|After receiving:||The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.|
|1 mg||5 mg||10 mg||20 mg||25 mg|
|1 mM||1.5711 mL||7.8555 mL||15.7109 mL||31.4218 mL||39.2773 mL|
|5 mM||0.3142 mL||1.5711 mL||3.1422 mL||6.2844 mL||7.8555 mL|
|10 mM||0.1571 mL||0.7855 mL||1.5711 mL||3.1422 mL||3.9277 mL|
|50 mM||0.0314 mL||0.1571 mL||0.3142 mL||0.6284 mL||0.7855 mL|
|100 mM||0.0157 mL||0.0786 mL||0.1571 mL||0.3142 mL||0.3928 mL|
Chinese Pharmaceutical Journal, 2015,50(19):1673-7.
|Study on the anti-inflammatory active constituents of Mangifera indica L. seed kernel.[Reference: WebLink]|
|To study the anti-inflammatory active constituents of Mangifera indica L. seed kernel. METHODS: The compounds were isolated by silica gel column chromatography, Sephadex LH-20 column chromatography and ODS column chromatography.Their structures were elucidated by spectral analyses and physicochemical properties. The anti-inflammatory activities of selected isolated compounds were evaluated as inhibitory activities against lipopolysaccharide (LPS)-induced nitric oxide (NO) production in RAW264.7 cell lines by Griess reaction. RESULTS: Eight compounds were obtained from n-butanol fraction oi Mangifera indica L. seed kernel, whose structures were elucidated as 4-O-ethylgallic acid (1), gallic acid (2), citric acid(3), 1,2,3,4,6-penta-O-galloylglucose(4),1,3,6-Tri-O-galloylglucose(5), hyperoside(6), quercetin-3-O-rhamnopyranoside(7), and mangiferin(8). Compounds 1, 2 and 4-8 exhibited potent inhibitory effect on NO production in LPS-induced macrophages, especially compounds 6 and 8 showed the best inhibitory activity with IC50 values of 16.5 and 19.5 μmol·L-1, respectively. CONCLUSION: Compounds 1 and 3-8 were obtained from Mangifera indica L.seed kernel for the first time.Compounds 1, 2 and 4-8 are the anti-inflammatory active constituents oi Mangifera indica L.seed kernel.Compounds 6 and 8 are the most potent anti-inflammatory active constituents.|