|Chinese Journal of Organic Chemistry, 2003, 23(8): 853-7. |
|Chemical constituents of the fungus Leccinum extremiorientale.[Reference: WebLink]|
METHODS AND RESULTS:
Thirteen chemical constituents were isolated from the fruiting bodies of basidiomyctes Leccinum extremiorientale, and their structures were elucidated by spectral and physical-chemical data as: ergosta-7,22-dien3β,5α ,6β-triol (1), ergosterol peroxide (2), ergosterol (3), ergosta-4,6,8(14),22-tetraen-3-one (4), ergosta-5,7-dien-3β-ol (5), palmitic acid (6), (2S,3S,4R,2′R )-2-(2'-Hydroxytetracosanoylamino)-octadecane-1,3,4-triol (7), cerebroside B (8), cerebroside D (9), uracil (10), inosine (11), adeosine (12) and D-allitol (13). The NMR data of compounds 8 and 9 were completely assigned by 2D-NMR techniques, including 1H- 1H COSY, HMBC and HMQC.
Compounds 2, 7 and 8 at concentration of 100 μg/mL showed selectively inhibitory activity against phospholipase A 2 (PLA2) secreted from Crotalus adamenteus venom, but inactive against PLA2 of bee venom (Apis mellifcra). In addition, the methanol-chloroform soluble extract of this fungus also showed 67% antifeedant rate against Leucaninia separata and caused 55% mortality against Plutella xylostella after three-day application.